The U1 snRNA gene repeat from the sea urchin (Stronglylocentrotus purpuratus) : the 70 kilobase tandem repeat ends directly 3' to a U1 gene
Lambda phage clones containing multiple copies of the 1.1 kb tandemly repeated unit of the sea urchin (S. purpuratus) U1 RNA genes were isolated from a gene library. The 1.1 kb repeat unit encodes a single copy of the predominant U1 RNA expressed in oocytes and embryos prior to the blastula stage. T...
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| Veröffentlicht in: | Nucleic acids research 1991-03, Vol.19 (5), p.1093-1098 |
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| creator | JIN-CHEN YU WENDELBURG, B SAKALLAH, S MARZLUFF, W. F |
| description | Lambda phage clones containing multiple copies of the 1.1 kb tandemly repeated unit of the sea urchin (S. purpuratus) U1 RNA genes were isolated from a gene library. The 1.1 kb repeat unit encodes a single copy of the predominant U1 RNA expressed in oocytes and embryos prior to the blastula stage. The tandem repeat unit is about 80 kb in size and is probably present one time per haploid genome as judged by pulsed-field electrophoresis of sperm DNA digested with restriction enzymes which do not cut in the repeat unit. Two of the phage contained DNA flanking the repeat unit as well as several repeat units. The tandem repeat unit ends just 3' to the U1 coding region. There is only limited homology in the 5' flanking region with U1 snRNA genes from the sea urchin L. variegatus. |
| doi_str_mv | 10.1093/nar/19.5.1093 |
| format | Article |
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F</creatorcontrib><title>The U1 snRNA gene repeat from the sea urchin (Stronglylocentrotus purpuratus) : the 70 kilobase tandem repeat ends directly 3' to a U1 gene</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Lambda phage clones containing multiple copies of the 1.1 kb tandemly repeated unit of the sea urchin (S. purpuratus) U1 RNA genes were isolated from a gene library. The 1.1 kb repeat unit encodes a single copy of the predominant U1 RNA expressed in oocytes and embryos prior to the blastula stage. The tandem repeat unit is about 80 kb in size and is probably present one time per haploid genome as judged by pulsed-field electrophoresis of sperm DNA digested with restriction enzymes which do not cut in the repeat unit. Two of the phage contained DNA flanking the repeat unit as well as several repeat units. The tandem repeat unit ends just 3' to the U1 coding region. There is only limited homology in the 5' flanking region with U1 snRNA genes from the sea urchin L. variegatus.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>DNA</subject><subject>Echinoidea</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes. Genome</subject><subject>Marine</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA, Small Nuclear - genetics</subject><subject>Sea Urchins</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>Strongylocentrotus purpuratus</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUktv1DAQthBVWQpHjki-8Ogh7diO4xiJQ1XRglSBBO3ZcpzJbiCxt3aCtL-BP413uxQ4VbLlGX-P8chDyAsGJwy0OPU2njJ9InfZI7JgouJFqSv-mCxAgCwYlPUT8jSl7wCsZLI8JIccOMiyWpBf1yukN4wm__XzGV2iRxpxjXaiXQwjnTKa0NI5ulXv6dtvUwx-OWyG4NDneJoTXc8xL5vDY_pup1BAf_RDaGxCOlnf4vjHFH2baNtHdNOwoeINnQK12_rbys_IQWeHhM_35xG5ufhwff6xuPpy-en87KpwpRJToRpQtUSmZVvrSisFTlWK5ytAJ6FjrbbMYdlxwSXDrmnBaa4scMe5bhpxRN7f-a7nZsR214kdzDr2o40bE2xv_kd8vzLL8NMIIVRdZf3rvT6G2xnTZMY-ORwG6zHMydQged7lg0Smaw2a1Q8TpS5rJVQmFndEF0NKEbv7VzMw2wEweRyyr5G7LPNf_tvqPXv__xl_tcdtcnboovWuT39NdZVb1rX4Dd7evfQ</recordid><startdate>19910311</startdate><enddate>19910311</enddate><creator>JIN-CHEN YU</creator><creator>WENDELBURG, B</creator><creator>SAKALLAH, S</creator><creator>MARZLUFF, W. 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| ispartof | Nucleic acids research, 1991-03, Vol.19 (5), p.1093-1098 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Base Sequence Biological and medical sciences Biological Evolution DNA Echinoidea Fundamental and applied biological sciences. Psychology Gene Library Genes. Genome Marine Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Repetitive Sequences, Nucleic Acid RNA, Small Nuclear - genetics Sea Urchins Sequence Homology, Nucleic Acid Species Specificity Strongylocentrotus purpuratus |
| title | The U1 snRNA gene repeat from the sea urchin (Stronglylocentrotus purpuratus) : the 70 kilobase tandem repeat ends directly 3' to a U1 gene |
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