Direct Observation of Nanoparticle–Cancer Cell Nucleus Interactions
We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope...
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| Veröffentlicht in: | ACS Nano 2012-04, Vol.6 (4), p.3318-3326 |
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| container_title | ACS Nano |
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| creator | Dam, Duncan Hieu M Lee, Jung Heon Sisco, Patrick N Co, Dick T Zhang, Ming Wasielewski, Michael R Odom, Teri W |
| description | We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy. |
| doi_str_mv | 10.1021/nn300296p |
| format | Article |
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Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/nn300296p</identifier><identifier>PMID: 22424173</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aptamers, Nucleotide - genetics ; Aptamers, Nucleotide - metabolism ; Base Sequence ; Cancer ; catalysis (homogeneous), catalysis (heterogeneous), solar (photovoltaic), solar (fuels), photosynthesis (natural and artificial), bio-inspired, hydrogen and fuel cells, electrodes - solar, defects, charge transport, spin dynamics, membrane, materials and chemistry by design, optics, synthesis (novel materials), synthesis (self-assembly) ; Cell Nucleus - metabolism ; Cell Nucleus - radiation effects ; Correlation ; Effectiveness ; Envelopes ; Gold ; Gold - chemistry ; Gold - metabolism ; HeLa Cells ; Humans ; Light ; Metal Nanoparticles - chemistry ; Molecular Imaging ; Nanoparticles ; Nanostructure ; Nuclear Envelope - metabolism ; Nuclear Envelope - radiation effects ; Nuclei ; Nucleolin ; Phosphoproteins - metabolism ; RNA-Binding Proteins - metabolism ; Surface chemistry ; Surface Properties</subject><ispartof>ACS Nano, 2012-04, Vol.6 (4), p.3318-3326</ispartof><rights>Copyright © 2012 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a531t-427ae1496ca5d411124c2c3a0be81dee3025ec6c0c64cc35febfc78ce8a806a33</citedby><cites>FETCH-LOGICAL-a531t-427ae1496ca5d411124c2c3a0be81dee3025ec6c0c64cc35febfc78ce8a806a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nn300296p$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nn300296p$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22424173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1066701$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Dam, Duncan Hieu M</creatorcontrib><creatorcontrib>Lee, Jung Heon</creatorcontrib><creatorcontrib>Sisco, Patrick N</creatorcontrib><creatorcontrib>Co, Dick T</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Wasielewski, Michael R</creatorcontrib><creatorcontrib>Odom, Teri W</creatorcontrib><creatorcontrib>Energy Frontier Research Centers (EFRC)</creatorcontrib><creatorcontrib>Argonne-Northwestern Solar Energy Research Center (ANSER)</creatorcontrib><title>Direct Observation of Nanoparticle–Cancer Cell Nucleus Interactions</title><title>ACS Nano</title><addtitle>ACS Nano</addtitle><description>We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.</description><subject>Aptamers, Nucleotide - genetics</subject><subject>Aptamers, Nucleotide - metabolism</subject><subject>Base Sequence</subject><subject>Cancer</subject><subject>catalysis (homogeneous), catalysis (heterogeneous), solar (photovoltaic), solar (fuels), photosynthesis (natural and artificial), bio-inspired, hydrogen and fuel cells, electrodes - solar, defects, charge transport, spin dynamics, membrane, materials and chemistry by design, optics, synthesis (novel materials), synthesis (self-assembly)</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - radiation effects</subject><subject>Correlation</subject><subject>Effectiveness</subject><subject>Envelopes</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gold - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Light</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Molecular Imaging</subject><subject>Nanoparticles</subject><subject>Nanostructure</subject><subject>Nuclear Envelope - metabolism</subject><subject>Nuclear Envelope - radiation effects</subject><subject>Nuclei</subject><subject>Nucleolin</subject><subject>Phosphoproteins - metabolism</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Surface chemistry</subject><subject>Surface Properties</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkd9qVDEQxoNY7B-98AXkIAj1YjWTnOScvSnIttpCaW8UvAuzs3NsytlkTXIKvfMdfEOfxJSti4JXEya_fPNlPiFegnwHUsH7ELSUam43T8QBzLWdyd5-fbo7G9gXhznfSmm6vrPPxL5SrWqh0wfi7NQnptJcLzOnOyw-hiYOzRWGuMFUPI3868fPBQbi1Cx4HJurqfam3FyEwgnp4UV-LvYGHDO_eKxH4svHs8-L89nl9aeLxYfLGRoNZdaqDhnauSU0qxYAVEuKNMol97Bi1lIZJkuSbEukzcDLgbqeuMdeWtT6SJxsdTfTcs0r4lASjm6T_BrTvYvo3b83wd-4b_HOaa07bdoq8HorEHPxLpMvTDcUQ6g7cCCt7SRU6PhxSorfJ87FrX2m-ncMHKfsoLNKGgnKVPTtFqUUc0487LyAdA_ZuF02lX31t_kd-SeMCrzZAkjZ3cYphbrL_wj9BtVtl64</recordid><startdate>20120424</startdate><enddate>20120424</enddate><creator>Dam, Duncan Hieu M</creator><creator>Lee, Jung Heon</creator><creator>Sisco, Patrick N</creator><creator>Co, Dick T</creator><creator>Zhang, Ming</creator><creator>Wasielewski, Michael R</creator><creator>Odom, Teri W</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20120424</creationdate><title>Direct Observation of Nanoparticle–Cancer Cell Nucleus Interactions</title><author>Dam, Duncan Hieu M ; Lee, Jung Heon ; Sisco, Patrick N ; Co, Dick T ; Zhang, Ming ; Wasielewski, Michael R ; Odom, Teri W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a531t-427ae1496ca5d411124c2c3a0be81dee3025ec6c0c64cc35febfc78ce8a806a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aptamers, Nucleotide - genetics</topic><topic>Aptamers, Nucleotide - metabolism</topic><topic>Base Sequence</topic><topic>Cancer</topic><topic>catalysis (homogeneous), catalysis (heterogeneous), solar (photovoltaic), solar (fuels), photosynthesis (natural and artificial), bio-inspired, hydrogen and fuel cells, electrodes - solar, defects, charge transport, spin dynamics, membrane, materials and chemistry by design, optics, synthesis (novel materials), synthesis (self-assembly)</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - radiation effects</topic><topic>Correlation</topic><topic>Effectiveness</topic><topic>Envelopes</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Gold - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Light</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Molecular Imaging</topic><topic>Nanoparticles</topic><topic>Nanostructure</topic><topic>Nuclear Envelope - metabolism</topic><topic>Nuclear Envelope - radiation effects</topic><topic>Nuclei</topic><topic>Nucleolin</topic><topic>Phosphoproteins - metabolism</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Surface chemistry</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dam, Duncan Hieu M</creatorcontrib><creatorcontrib>Lee, Jung Heon</creatorcontrib><creatorcontrib>Sisco, Patrick N</creatorcontrib><creatorcontrib>Co, Dick T</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Wasielewski, Michael R</creatorcontrib><creatorcontrib>Odom, Teri W</creatorcontrib><creatorcontrib>Energy Frontier Research Centers (EFRC)</creatorcontrib><creatorcontrib>Argonne-Northwestern Solar Energy Research Center (ANSER)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>ACS Nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dam, Duncan Hieu M</au><au>Lee, Jung Heon</au><au>Sisco, Patrick N</au><au>Co, Dick T</au><au>Zhang, Ming</au><au>Wasielewski, Michael R</au><au>Odom, Teri W</au><aucorp>Energy Frontier Research Centers (EFRC)</aucorp><aucorp>Argonne-Northwestern Solar Energy Research Center (ANSER)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct Observation of Nanoparticle–Cancer Cell Nucleus Interactions</atitle><jtitle>ACS Nano</jtitle><addtitle>ACS Nano</addtitle><date>2012-04-24</date><risdate>2012</risdate><volume>6</volume><issue>4</issue><spage>3318</spage><epage>3326</epage><pages>3318-3326</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>We report the direct visualization of interactions between drug-loaded nanoparticles and the cancer cell nucleus. Nanoconstructs composed of nucleolin-specific aptamers and gold nanostars were actively transported to the nucleus and induced major changes to the nuclear phenotype via nuclear envelope invaginations near the site of the construct. The number of local deformations could be increased by ultrafast, light-triggered release of the aptamers from the surface of the gold nanostars. Cancer cells with more nuclear envelope folding showed increased caspase 3 and 7 activity (apoptosis) as well as decreased cell viability. This newly revealed correlation between drug-induced changes in nuclear phenotype and increased therapeutic efficacy could provide new insight for nuclear-targeted cancer therapy.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>22424173</pmid><doi>10.1021/nn300296p</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aptamers, Nucleotide - genetics Aptamers, Nucleotide - metabolism Base Sequence Cancer catalysis (homogeneous), catalysis (heterogeneous), solar (photovoltaic), solar (fuels), photosynthesis (natural and artificial), bio-inspired, hydrogen and fuel cells, electrodes - solar, defects, charge transport, spin dynamics, membrane, materials and chemistry by design, optics, synthesis (novel materials), synthesis (self-assembly) Cell Nucleus - metabolism Cell Nucleus - radiation effects Correlation Effectiveness Envelopes Gold Gold - chemistry Gold - metabolism HeLa Cells Humans Light Metal Nanoparticles - chemistry Molecular Imaging Nanoparticles Nanostructure Nuclear Envelope - metabolism Nuclear Envelope - radiation effects Nuclei Nucleolin Phosphoproteins - metabolism RNA-Binding Proteins - metabolism Surface chemistry Surface Properties |
| title | Direct Observation of Nanoparticle–Cancer Cell Nucleus Interactions |
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