Protein-DNA cross-linking at the lac promoter

We report the results of photo-cross-linking of RNA polymerase and the cyclic AMP receptor protein (CRP) to the lac UV5 promoter region carried on either a linear fragment or a supercoiled plasmid. We have devised a protocol that allows the localisation of bases in contact with the protein. RNA poly...

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Veröffentlicht in:	Nucleic acids research 1991-02, Vol.19 (4), p.833-840
Hauptverfasser:	BUCKLE, M, GEISELMANN, J, KOLB, A, BUC, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Cross-Linking Reagents DNA - radiation effects DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism DNA-Directed RNA Polymerases - chemistry Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Promoter Regions, Genetic Repressor Proteins - genetics Transcription. Transcription factor. Splicing. Rna processing
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