Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic cells and murine embryonic and adult tissues
The HOX 2.2 homeobox gene is expressed in human hematopoietic cell lines with erythroid features (W.-F. Shen, et al, Proc. Natl. Acad. Sci. 86, 8536-8540, 1989). Both human and murine Hox 2.2 genes contain a single 1 kb intron which interrupts the sequence encoding the proposed homeobox protein. Fou...
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| Veröffentlicht in: | Nucleic acids research 1991-02, Vol.19 (3), p.539-545 |
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| creator | Shen, W F Detmer, K Simonitch-Eason, T A Lawrence, H J Largman, C |
| description | The HOX 2.2 homeobox gene is expressed in human hematopoietic cell lines with erythroid features (W.-F. Shen, et al, Proc. Natl. Acad. Sci. 86, 8536-8540, 1989). Both human and murine Hox 2.2 genes contain a single 1 kb intron which interrupts the sequence encoding the proposed homeobox protein. Four human erythroleukemia cell lines express the spliced, homeobox-coding transcript as the major form of message, and variable low amounts of unspliced HOX 2.2 mRNAs. Murine embryonic tissues and adult kidney and uterus contain approximately equal amounts of transcripts containing this intron and mRNAs from which the intron has been excised. The spliced transcript encodes a 224 amino acid homeobox protein, while the unspliced transcript would potentially encode a 140 residue protein containing the same N-terminal sequence but lacking the homeodomain. |
| doi_str_mv | 10.1093/nar/19.3.539 |
| format | Article |
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Shen, et al, Proc. Natl. Acad. Sci. 86, 8536-8540, 1989). Both human and murine Hox 2.2 genes contain a single 1 kb intron which interrupts the sequence encoding the proposed homeobox protein. Four human erythroleukemia cell lines express the spliced, homeobox-coding transcript as the major form of message, and variable low amounts of unspliced HOX 2.2 mRNAs. Murine embryonic tissues and adult kidney and uterus contain approximately equal amounts of transcripts containing this intron and mRNAs from which the intron has been excised. The spliced transcript encodes a 224 amino acid homeobox protein, while the unspliced transcript would potentially encode a 140 residue protein containing the same N-terminal sequence but lacking the homeodomain.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/19.3.539</identifier><identifier>PMID: 1672751</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; DNA - genetics ; Genes, Homeobox ; Hematopoietic Stem Cells - physiology ; Humans ; Introns ; K562 cells ; Leukemia, Erythroblastic, Acute - genetics ; Mice ; Molecular Sequence Data ; mRNA ; Oligonucleotides - chemistry ; RNA Splicing ; RNA, Messenger - genetics ; Tumor Cells, Cultured</subject><ispartof>Nucleic acids research, 1991-02, Vol.19 (3), p.539-545</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-572c97057b2c9c5feb470054bd8934f9650688a978565c55122a1acb4ea7534a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC333645/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC333645/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1672751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, W F</creatorcontrib><creatorcontrib>Detmer, K</creatorcontrib><creatorcontrib>Simonitch-Eason, T A</creatorcontrib><creatorcontrib>Lawrence, H J</creatorcontrib><creatorcontrib>Largman, C</creatorcontrib><title>Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic cells and murine embryonic and adult tissues</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The HOX 2.2 homeobox gene is expressed in human hematopoietic cell lines with erythroid features (W.-F. 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The spliced transcript encodes a 224 amino acid homeobox protein, while the unspliced transcript would potentially encode a 140 residue protein containing the same N-terminal sequence but lacking the homeodomain.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>DNA - genetics</subject><subject>Genes, Homeobox</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Humans</subject><subject>Introns</subject><subject>K562 cells</subject><subject>Leukemia, Erythroblastic, Acute - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>mRNA</subject><subject>Oligonucleotides - chemistry</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LJDEQhsOyorPqba9CTnuyx3x2OgcPIu66IHhR8BbSmeqZLN3JmKRF__1mGNmPk5cUpJ56qeJB6CslS0o0vwg2XVC95EvJ9Se0oLxljdAt-4wWhBPZUCK6I_Ql51-EUEGlOESHtFVMSbpA89VYIAVb_AvgvB2982GN44DLBvDt_RNmS4Y3cYLYx1e8hgDYB7yZJ1tfmGyJ2-iheIcdjGPGNqzwNCdfOZj69BZDbe0-7WoeCy4-5xnyCToY7Jjh9L0eo8fvNw_Xt83d_Y-f11d3jZOkLY1UzGlFpOprdXKAXihCpOhXneZi0G2lus5q1clWOikpY5Za1wuwSnJh-TG63Odu536ClYNQkh3NNvnJpjcTrTf_d4LfmHV8MZzzVsg6_-19PsXnuncxk8-7Q22AOGfTEaGVUh-DtPpglIuPQakZI3SXeL4HXYo5Jxj-bE2J2Xk31XvNNdxU7xU_-_fSv_BeNP8NuGmqHA</recordid><startdate>19910211</startdate><enddate>19910211</enddate><creator>Shen, W F</creator><creator>Detmer, K</creator><creator>Simonitch-Eason, T A</creator><creator>Lawrence, H J</creator><creator>Largman, C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910211</creationdate><title>Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic cells and murine embryonic and adult tissues</title><author>Shen, W F ; Detmer, K ; Simonitch-Eason, T A ; Lawrence, H J ; Largman, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-572c97057b2c9c5feb470054bd8934f9650688a978565c55122a1acb4ea7534a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>DNA - genetics</topic><topic>Genes, Homeobox</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Humans</topic><topic>Introns</topic><topic>K562 cells</topic><topic>Leukemia, Erythroblastic, Acute - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>mRNA</topic><topic>Oligonucleotides - chemistry</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, W F</creatorcontrib><creatorcontrib>Detmer, K</creatorcontrib><creatorcontrib>Simonitch-Eason, T A</creatorcontrib><creatorcontrib>Lawrence, H J</creatorcontrib><creatorcontrib>Largman, C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, W F</au><au>Detmer, K</au><au>Simonitch-Eason, T A</au><au>Lawrence, H J</au><au>Largman, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic cells and murine embryonic and adult tissues</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-02-11</date><risdate>1991</risdate><volume>19</volume><issue>3</issue><spage>539</spage><epage>545</epage><pages>539-545</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The HOX 2.2 homeobox gene is expressed in human hematopoietic cell lines with erythroid features (W.-F. Shen, et al, Proc. Natl. Acad. Sci. 86, 8536-8540, 1989). Both human and murine Hox 2.2 genes contain a single 1 kb intron which interrupts the sequence encoding the proposed homeobox protein. Four human erythroleukemia cell lines express the spliced, homeobox-coding transcript as the major form of message, and variable low amounts of unspliced HOX 2.2 mRNAs. Murine embryonic tissues and adult kidney and uterus contain approximately equal amounts of transcripts containing this intron and mRNAs from which the intron has been excised. The spliced transcript encodes a 224 amino acid homeobox protein, while the unspliced transcript would potentially encode a 140 residue protein containing the same N-terminal sequence but lacking the homeodomain.</abstract><cop>England</cop><pmid>1672751</pmid><doi>10.1093/nar/19.3.539</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1991-02, Vol.19 (3), p.539-545 |
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| language | eng |
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| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amino Acid Sequence Animals Base Sequence Blotting, Northern DNA - genetics Genes, Homeobox Hematopoietic Stem Cells - physiology Humans Introns K562 cells Leukemia, Erythroblastic, Acute - genetics Mice Molecular Sequence Data mRNA Oligonucleotides - chemistry RNA Splicing RNA, Messenger - genetics Tumor Cells, Cultured |
| title | Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic cells and murine embryonic and adult tissues |
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