Studies on conjugation of Spirogyra using monoclonal culture
We succeeded in inducing conjugation of Spirogyra castanacea by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all...
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| Veröffentlicht in: | Journal of plant research 2012-05, Vol.125 (3), p.457-464 |
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| creator | Ikegaya, Hisato Nakase, Takuto Iwata, Kazuyoshi Tsuchida, Hideaki Sonobe, Seiji Shimmen, Teruo |
| description | We succeeded in inducing conjugation of
Spirogyra
castanacea
by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all cells of one filament behaved as male and those of other filament did as female. Very rarely, however, zygospores were formed in both of pair filaments. The surface of conjugation tube was stained with fluorescently labeled-lectins, such as
Bandeiraea
(
Griffonia
)
simplicifolia
lectin (BSL-I) and jacalin. BSL-I strongly stained the conjugation tubes, while weakly did the cell surface of female gamete first and then that of male gamete. Jacalin stained mainly the conjugation tubes. Addition of jacalin inhibited the formation of papilla, suggesting some important role of jacalin-binding material at the initial step of formation of the conjugation tubes. |
| doi_str_mv | 10.1007/s10265-011-0457-3 |
| format | Article |
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Spirogyra
castanacea
by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all cells of one filament behaved as male and those of other filament did as female. Very rarely, however, zygospores were formed in both of pair filaments. The surface of conjugation tube was stained with fluorescently labeled-lectins, such as
Bandeiraea
(
Griffonia
)
simplicifolia
lectin (BSL-I) and jacalin. BSL-I strongly stained the conjugation tubes, while weakly did the cell surface of female gamete first and then that of male gamete. Jacalin stained mainly the conjugation tubes. Addition of jacalin inhibited the formation of papilla, suggesting some important role of jacalin-binding material at the initial step of formation of the conjugation tubes.</description><identifier>ISSN: 0918-9440</identifier><identifier>EISSN: 1618-0860</identifier><identifier>DOI: 10.1007/s10265-011-0457-3</identifier><identifier>PMID: 22006213</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Agar ; Algae ; Aquatic Organisms - physiology ; Biomedical and Life Sciences ; Cell culture ; Cell cycle ; Cell surface ; Filaments ; Gametes ; Germ Cells, Plant - cytology ; Germ Cells, Plant - growth & development ; Griffonia simplicifolia ; Lectins ; Life Sciences ; Plant Biochemistry ; Plant Ecology ; Plant Growth Regulators - metabolism ; Plant Lectins - metabolism ; Plant Physiology ; Plant reproduction ; Plant Sciences ; Regular Paper ; Reproduction - physiology ; Spirogyra ; Spirogyra - cytology ; Spirogyra - growth & development ; Zygospores</subject><ispartof>Journal of plant research, 2012-05, Vol.125 (3), p.457-464</ispartof><rights>The Author(s) 2011</rights><rights>The Botanical Society of Japan and Springer 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-5304943f2de6a8500aa2069f9d42d5c70ea25eacc9e49d637b714bd1aa0429f43</citedby><cites>FETCH-LOGICAL-c596t-5304943f2de6a8500aa2069f9d42d5c70ea25eacc9e49d637b714bd1aa0429f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10265-011-0457-3$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10265-011-0457-3$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22006213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikegaya, Hisato</creatorcontrib><creatorcontrib>Nakase, Takuto</creatorcontrib><creatorcontrib>Iwata, Kazuyoshi</creatorcontrib><creatorcontrib>Tsuchida, Hideaki</creatorcontrib><creatorcontrib>Sonobe, Seiji</creatorcontrib><creatorcontrib>Shimmen, Teruo</creatorcontrib><title>Studies on conjugation of Spirogyra using monoclonal culture</title><title>Journal of plant research</title><addtitle>J Plant Res</addtitle><addtitle>J Plant Res</addtitle><description>We succeeded in inducing conjugation of
Spirogyra
castanacea
by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all cells of one filament behaved as male and those of other filament did as female. Very rarely, however, zygospores were formed in both of pair filaments. The surface of conjugation tube was stained with fluorescently labeled-lectins, such as
Bandeiraea
(
Griffonia
)
simplicifolia
lectin (BSL-I) and jacalin. BSL-I strongly stained the conjugation tubes, while weakly did the cell surface of female gamete first and then that of male gamete. Jacalin stained mainly the conjugation tubes. Addition of jacalin inhibited the formation of papilla, suggesting some important role of jacalin-binding material at the initial step of formation of the conjugation tubes.</description><subject>Agar</subject><subject>Algae</subject><subject>Aquatic Organisms - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell surface</subject><subject>Filaments</subject><subject>Gametes</subject><subject>Germ Cells, Plant - cytology</subject><subject>Germ Cells, Plant - growth & development</subject><subject>Griffonia simplicifolia</subject><subject>Lectins</subject><subject>Life Sciences</subject><subject>Plant Biochemistry</subject><subject>Plant Ecology</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Lectins - metabolism</subject><subject>Plant Physiology</subject><subject>Plant reproduction</subject><subject>Plant Sciences</subject><subject>Regular Paper</subject><subject>Reproduction - physiology</subject><subject>Spirogyra</subject><subject>Spirogyra - cytology</subject><subject>Spirogyra - growth & development</subject><subject>Zygospores</subject><issn>0918-9440</issn><issn>1618-0860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkU1rGzEQhkVJaRy3P6CXsJBLL5uMvldQAsG0SSDQg5OzkLXazZq15EqrQP59ZeyGtBDISQPz6NFoXoS-YjjHAPIiYSCC14BxDYzLmn5AMyxwU0Mj4AjNQJVaMQbH6CSlNQCWXDWf0DEhAIJgOkPfl1NuB5eq4Csb_Dr3ZhpKHbpquR1i6J-jqXIafF9tgg92DN6Mlc3jlKP7jD52Zkzuy-Gco4efP-4XN_Xdr-vbxdVdbbkSU80pMMVoR1onTMMBjCEgVKdaRlpuJThDuDPWKsdUK6hcScxWLTYGGFEdo3N0ufdu82rjWuv8FM2ot3HYmPisgxn0vx0_POo-PGlKqQAORfDtIIjhd3Zp0pshWTeOxruQky57bAA3uHkPCooTKSQv6Nl_6DrkWPazozBljEoqCoX3lI0hpei6l7kx7GxS72PUJUa9i7FMPUenrz_8cuNvbgUgeyCVlu9dfP30W9Y_hjCnaA</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Ikegaya, Hisato</creator><creator>Nakase, Takuto</creator><creator>Iwata, Kazuyoshi</creator><creator>Tsuchida, Hideaki</creator><creator>Sonobe, Seiji</creator><creator>Shimmen, Teruo</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7ST</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20120501</creationdate><title>Studies on conjugation of Spirogyra using monoclonal culture</title><author>Ikegaya, Hisato ; Nakase, Takuto ; Iwata, Kazuyoshi ; Tsuchida, Hideaki ; Sonobe, Seiji ; Shimmen, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-5304943f2de6a8500aa2069f9d42d5c70ea25eacc9e49d637b714bd1aa0429f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agar</topic><topic>Algae</topic><topic>Aquatic Organisms - 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Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of plant research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikegaya, Hisato</au><au>Nakase, Takuto</au><au>Iwata, Kazuyoshi</au><au>Tsuchida, Hideaki</au><au>Sonobe, Seiji</au><au>Shimmen, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on conjugation of Spirogyra using monoclonal culture</atitle><jtitle>Journal of plant research</jtitle><stitle>J Plant Res</stitle><addtitle>J Plant Res</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>125</volume><issue>3</issue><spage>457</spage><epage>464</epage><pages>457-464</pages><issn>0918-9440</issn><eissn>1618-0860</eissn><abstract>We succeeded in inducing conjugation of
Spirogyra
castanacea
by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all cells of one filament behaved as male and those of other filament did as female. Very rarely, however, zygospores were formed in both of pair filaments. The surface of conjugation tube was stained with fluorescently labeled-lectins, such as
Bandeiraea
(
Griffonia
)
simplicifolia
lectin (BSL-I) and jacalin. BSL-I strongly stained the conjugation tubes, while weakly did the cell surface of female gamete first and then that of male gamete. Jacalin stained mainly the conjugation tubes. Addition of jacalin inhibited the formation of papilla, suggesting some important role of jacalin-binding material at the initial step of formation of the conjugation tubes.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>22006213</pmid><doi>10.1007/s10265-011-0457-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0918-9440 |
| ispartof | Journal of plant research, 2012-05, Vol.125 (3), p.457-464 |
| issn | 0918-9440 1618-0860 |
| language | eng |
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| subjects | Agar Algae Aquatic Organisms - physiology Biomedical and Life Sciences Cell culture Cell cycle Cell surface Filaments Gametes Germ Cells, Plant - cytology Germ Cells, Plant - growth & development Griffonia simplicifolia Lectins Life Sciences Plant Biochemistry Plant Ecology Plant Growth Regulators - metabolism Plant Lectins - metabolism Plant Physiology Plant reproduction Plant Sciences Regular Paper Reproduction - physiology Spirogyra Spirogyra - cytology Spirogyra - growth & development Zygospores |
| title | Studies on conjugation of Spirogyra using monoclonal culture |
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