U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems
The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1991-01, Vol.19 (1), p.1-4 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4 |
|---|---|
| container_issue | 1 |
| container_start_page | 1 |
| container_title | Nucleic acids research |
| container_volume | 19 |
| creator | Witmer, M R Falcomer, C M Weiner, M P Kay, M S Begley, T P Ganem, B Scheraga, H A |
| description | The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods. |
| doi_str_mv | 10.1093/nar/19.1.1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_333526</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80497385</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-e7c8721346e02ad2128897c7feb8bbb4f28db45b3f6b43c75de3746c9fff25893</originalsourceid><addsrcrecordid>eNqFkcFrFDEUh4NY6lq9eBdysiDMNi_JzCSCh-2qtVCqiD2HJPPSHdlJ1iQr9r93SpeiJ0_v8L7f4_f4CHkFbAlMi7No8xnoJSzhCVmA6HgjdcefkgUTrG2ASfWMPC_lB2MgoZXH5JgzAKn0guBNI06b8_Xl13fUUr_JaUq3GEdPy96Vmm1FGlKmdYN0wIq-jinSFOi3a1uQrugYaUafJjdGGyv9cL2i-HuXsZR7sNyVilN5QY6C3RZ8eZgn5ObTx-_rz83Vl4vL9eqq8ZKJ2mDvVc9ByA4ZtwMHrpTufR_QKeecDFwNTrZOhM5J4ft2QNHLzusQAm-VFifk_cPd3d5NOHiM8wdbs8vjZPOdSXY0_27iuDG36ZcRQrS8m_NvDvmcfu6xVDONxeN2ayOmfTGKSd0L1f4XBC06kFzO4NsH0OdUSsbwWAaYuZdnZnkzbsDADL_-u_4jerAl_gBrI5Xc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19361424</pqid></control><display><type>article</type><title>U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems</title><source>MEDLINE</source><source>PubMed Central</source><source>Oxford University Press Journals Digital Archive Legacy</source><creator>Witmer, M R ; Falcomer, C M ; Weiner, M P ; Kay, M S ; Begley, T P ; Ganem, B ; Scheraga, H A</creator><creatorcontrib>Witmer, M R ; Falcomer, C M ; Weiner, M P ; Kay, M S ; Begley, T P ; Ganem, B ; Scheraga, H A</creatorcontrib><description>The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/19.1.1</identifier><identifier>PMID: 2011489</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Cattle ; DNA, Recombinant ; Kinetics ; Molecular Structure ; Oxidation-Reduction ; Ribonuclease, Pancreatic - analysis ; Ribonuclease, Pancreatic - genetics ; Ribonuclease, Pancreatic - metabolism ; Substrate Specificity ; Uridine Monophosphate - analogs & derivatives ; Uridine Monophosphate - chemical synthesis ; Uridine Monophosphate - metabolism</subject><ispartof>Nucleic acids research, 1991-01, Vol.19 (1), p.1-4</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-e7c8721346e02ad2128897c7feb8bbb4f28db45b3f6b43c75de3746c9fff25893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC333526/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC333526/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27902,27903,53768,53770</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2011489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Witmer, M R</creatorcontrib><creatorcontrib>Falcomer, C M</creatorcontrib><creatorcontrib>Weiner, M P</creatorcontrib><creatorcontrib>Kay, M S</creatorcontrib><creatorcontrib>Begley, T P</creatorcontrib><creatorcontrib>Ganem, B</creatorcontrib><creatorcontrib>Scheraga, H A</creatorcontrib><title>U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods.</description><subject>Animals</subject><subject>Cattle</subject><subject>DNA, Recombinant</subject><subject>Kinetics</subject><subject>Molecular Structure</subject><subject>Oxidation-Reduction</subject><subject>Ribonuclease, Pancreatic - analysis</subject><subject>Ribonuclease, Pancreatic - genetics</subject><subject>Ribonuclease, Pancreatic - metabolism</subject><subject>Substrate Specificity</subject><subject>Uridine Monophosphate - analogs & derivatives</subject><subject>Uridine Monophosphate - chemical synthesis</subject><subject>Uridine Monophosphate - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFrFDEUh4NY6lq9eBdysiDMNi_JzCSCh-2qtVCqiD2HJPPSHdlJ1iQr9r93SpeiJ0_v8L7f4_f4CHkFbAlMi7No8xnoJSzhCVmA6HgjdcefkgUTrG2ASfWMPC_lB2MgoZXH5JgzAKn0guBNI06b8_Xl13fUUr_JaUq3GEdPy96Vmm1FGlKmdYN0wIq-jinSFOi3a1uQrugYaUafJjdGGyv9cL2i-HuXsZR7sNyVilN5QY6C3RZ8eZgn5ObTx-_rz83Vl4vL9eqq8ZKJ2mDvVc9ByA4ZtwMHrpTufR_QKeecDFwNTrZOhM5J4ft2QNHLzusQAm-VFifk_cPd3d5NOHiM8wdbs8vjZPOdSXY0_27iuDG36ZcRQrS8m_NvDvmcfu6xVDONxeN2ayOmfTGKSd0L1f4XBC06kFzO4NsH0OdUSsbwWAaYuZdnZnkzbsDADL_-u_4jerAl_gBrI5Xc</recordid><startdate>19910111</startdate><enddate>19910111</enddate><creator>Witmer, M R</creator><creator>Falcomer, C M</creator><creator>Weiner, M P</creator><creator>Kay, M S</creator><creator>Begley, T P</creator><creator>Ganem, B</creator><creator>Scheraga, H A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910111</creationdate><title>U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems</title><author>Witmer, M R ; Falcomer, C M ; Weiner, M P ; Kay, M S ; Begley, T P ; Ganem, B ; Scheraga, H A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-e7c8721346e02ad2128897c7feb8bbb4f28db45b3f6b43c75de3746c9fff25893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>DNA, Recombinant</topic><topic>Kinetics</topic><topic>Molecular Structure</topic><topic>Oxidation-Reduction</topic><topic>Ribonuclease, Pancreatic - analysis</topic><topic>Ribonuclease, Pancreatic - genetics</topic><topic>Ribonuclease, Pancreatic - metabolism</topic><topic>Substrate Specificity</topic><topic>Uridine Monophosphate - analogs & derivatives</topic><topic>Uridine Monophosphate - chemical synthesis</topic><topic>Uridine Monophosphate - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Witmer, M R</creatorcontrib><creatorcontrib>Falcomer, C M</creatorcontrib><creatorcontrib>Weiner, M P</creatorcontrib><creatorcontrib>Kay, M S</creatorcontrib><creatorcontrib>Begley, T P</creatorcontrib><creatorcontrib>Ganem, B</creatorcontrib><creatorcontrib>Scheraga, H A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Witmer, M R</au><au>Falcomer, C M</au><au>Weiner, M P</au><au>Kay, M S</au><au>Begley, T P</au><au>Ganem, B</au><au>Scheraga, H A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-01-11</date><risdate>1991</risdate><volume>19</volume><issue>1</issue><spage>1</spage><epage>4</epage><pages>1-4</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods.</abstract><cop>England</cop><pmid>2011489</pmid><doi>10.1093/nar/19.1.1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1991-01, Vol.19 (1), p.1-4 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_333526 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Animals Cattle DNA, Recombinant Kinetics Molecular Structure Oxidation-Reduction Ribonuclease, Pancreatic - analysis Ribonuclease, Pancreatic - genetics Ribonuclease, Pancreatic - metabolism Substrate Specificity Uridine Monophosphate - analogs & derivatives Uridine Monophosphate - chemical synthesis Uridine Monophosphate - metabolism |
| title | U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T08%3A52%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=U-3'-BCIP:%20a%20chromogenic%20substrate%20for%20the%20detection%20of%20RNase%20A%20in%20recombinant%20DNA%20expression%20systems&rft.jtitle=Nucleic%20acids%20research&rft.au=Witmer,%20M%20R&rft.date=1991-01-11&rft.volume=19&rft.issue=1&rft.spage=1&rft.epage=4&rft.pages=1-4&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/19.1.1&rft_dat=%3Cproquest_pubme%3E80497385%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19361424&rft_id=info:pmid/2011489&rfr_iscdi=true |