The osmolarity of the electroporation medium affects the transient expression of genes

During transfection on 5123 and FT02B hepatoma cells and NIH 3T3 fibroblast cells by electroporation, it was observed that the expression of the reporter gene and the viability of the cells was very sensitive to varying amounts of DNA solution added, despite a constant setting of voltage and capacit...

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Veröffentlicht in:	Nucleic acids research 1990-11, Vol.18 (21), p.6464-6464
Hauptverfasser:	VAN DEN HOFF, M. J. B, LABRUYERE, W. T, MOORMAN, A. F. M, LAMERS, W. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Avian Sarcoma Viruses - genetics Biological and medical sciences Biotechnology Cell Line Cell Survival Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - genetics Electric Stimulation - methods electroporation Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Techniques Methods. Procedures. Technologies Mice Osmolar Concentration Promoter Regions, Genetic Recombinant Fusion Proteins - biosynthesis Transfection
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