Improved High-Force Magnetic Tweezers for Stretching and Refolding of Proteins and Short DNA

Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows...

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Veröffentlicht in:	Biophysical journal 2011-01, Vol.100 (2), p.517-523
Hauptverfasser:	Chen, Hu, Fu, Hongxia, Zhu, Xiaoying, Cong, Peiwen, Nakamura, Fumihiko, Yan, Jie
Format:	Artikel
Sprache:	eng
Schlagworte:	Biophysics Calibration Contractile Proteins - chemistry Deoxyribonucleic acid DNA DNA - chemistry filamin Filamins Immunoglobulin Fragments - chemistry immunoglobulins Magnetics - instrumentation Magnetics - methods Microfilament Proteins - chemistry Optical Tweezers Protein Folding Spectroscopy, Imaging, and Other Techniques
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