Single-Image Diffusion Coefficient Measurements of Proteins in Free Solution
Diffusion coefficient measurements are important for many biological and material investigations, such as studies of particle dynamics and kinetics, and size determinations. Among current measurement methods, single particle tracking (SPT) offers the unique ability to simultaneously obtain location...
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| Veröffentlicht in: | Biophysical journal 2012-04, Vol.102 (7), p.1685-1691 |
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| creator | Zareh, Shannon Kian DeSantis, Michael C. Kessler, Jonathan M. Li, Je-Luen Wang, Y.M. |
| description | Diffusion coefficient measurements are important for many biological and material investigations, such as studies of particle dynamics and kinetics, and size determinations. Among current measurement methods, single particle tracking (SPT) offers the unique ability to simultaneously obtain location and diffusion information about a molecule while using only femtomoles of sample. However, the temporal resolution of SPT is limited to seconds for single-color-labeled samples. By directly imaging three-dimensional diffusing fluorescent proteins and studying the widths of their intensity profiles, we were able to determine the proteins' diffusion coefficients using single protein images of submillisecond exposure times. This simple method improves the temporal resolution of diffusion coefficient measurements to submilliseconds, and can be readily applied to a range of particle sizes in SPT investigations and applications in which diffusion coefficient measurements are needed, such as reaction kinetics and particle size determinations. |
| doi_str_mv | 10.1016/j.bpj.2012.02.030 |
| format | Article |
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This simple method improves the temporal resolution of diffusion coefficient measurements to submilliseconds, and can be readily applied to a range of particle sizes in SPT investigations and applications in which diffusion coefficient measurements are needed, such as reaction kinetics and particle size determinations.</description><subject>Diffusion</subject><subject>diffusivity</subject><subject>digital images</subject><subject>exposure duration</subject><subject>Fluorescence</subject><subject>fluorescent proteins</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>information dissemination</subject><subject>Molecular Imaging - methods</subject><subject>Molecules</subject><subject>Particle Size</subject><subject>Proteins</subject><subject>Reaction kinetics</subject><subject>Solutions</subject><subject>Solvents - chemistry</subject><subject>Spectroscopy, Imaging, and Other Techniques</subject><subject>Time Factors</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVGL1DAQx4Mo3t7pB_BFC77cS9eZpE1bBEFWTw9WFNZ7Dmk6WVO6zV7SHvjtTdnzUB-EgQnMb_6ZmT9jLxDWCCjf9Ov22K85IF9DCgGP2ArLgucAtXzMVgAgc1E05Rk7j7GHBJaAT9kZTxkq2azYdufG_UD59UHvKfvgrJ2j82O28WStM47GKftCOs6BDukdM2-zb8FP5MaYuTG7CkTZzg_zlLqesSdWD5Ge3-cLdnP18fvmc779-ul6836bmxLElEtbNyBkyxuQFbZGNIbb2vCyw6LWHZfQVZxbIytZ2brFFnVZiK5BARyoQnHB3p10j3N7oM6kwYIe1DG4gw4_lddO_V0Z3Q-193dKCKwRRRK4vBcI_namOKmDi4aGQY_k56gwnaqRUPDlr9f_oL2fw5jWWyieFAssE4UnygQfYyD7MAyCWrxSvUpeqcUrBSkEpJ6Xf27x0PHbnAS8OgFWe6X3wUV1s0sKqYzIRSMT8fZEULr2naOg4mKZoc4FMpPqvPvPAL8A6GusVQ</recordid><startdate>20120404</startdate><enddate>20120404</enddate><creator>Zareh, Shannon Kian</creator><creator>DeSantis, Michael C.</creator><creator>Kessler, Jonathan M.</creator><creator>Li, Je-Luen</creator><creator>Wang, Y.M.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120404</creationdate><title>Single-Image Diffusion Coefficient Measurements of Proteins in Free Solution</title><author>Zareh, Shannon Kian ; 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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Diffusion diffusivity digital images exposure duration Fluorescence fluorescent proteins Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism information dissemination Molecular Imaging - methods Molecules Particle Size Proteins Reaction kinetics Solutions Solvents - chemistry Spectroscopy, Imaging, and Other Techniques Time Factors |
| title | Single-Image Diffusion Coefficient Measurements of Proteins in Free Solution |
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