Cloning the BamHI restriction modification system

BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Although within the clone the methylase and endonucl...

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Veröffentlicht in:	Nucleic acids research 1989-02, Vol.17 (3), p.979-997
Hauptverfasser:	BROOKS, J. E, BENNER, J. S, HEITER, D. F, SILBER, K. R, SZNYTER, L. A, JAGER-QUINTON, T, MORAN, L. S, SLATKO, B. E, WILSON, G. G, NWANKWO, D. O
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus Bacillus - enzymology Bacillus - genetics Bacillus amyloliquefaciens Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Base Sequence Biological and medical sciences Cloning, Molecular - methods Deoxyribonuclease BamHI - genetics Deoxyribonuclease BamHI - isolation & purification Deoxyribonuclease BamHI - physiology DNA Modification Methylases - genetics DNA Probes - genetics DNA Probes - isolation & purification DNA Transposable Elements DNA, Bacterial - isolation & purification Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Rearrangement Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Restriction Mapping - methods Transformation, Genetic
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