Distribution of Cardiac Stem Cells in the Human Heart

Distribution of Cardiac Stem Cells in the Human Heart

Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the ri...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ISRN Cardiology 2012, Vol.2012 (2012), p.1-5
Hauptverfasser: Arsalan, Mani, Woitek, Felix, Adams, Volker, Linke, Axel, Barten, Markus J., Dhein, Stefan, Walther, Thomas, Mohr, Friedrich-Wilhelm, Garbade, Jens
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 5
container_issue 2012
container_start_page 1
container_title ISRN Cardiology
container_volume 2012
creator Arsalan, Mani
Woitek, Felix
Adams, Volker
Linke, Axel
Barten, Markus J.
Dhein, Stefan
Walther, Thomas
Mohr, Friedrich-Wilhelm
Garbade, Jens
description Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, P=0.922). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, P=0.035). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.
doi_str_mv 10.5402/2012/483407
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3312548</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>963493612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2827-a40c638762de0fb1ad122533d641ad1075b7b792b2ce23c8f438cee51fb9e29c3</originalsourceid><addsrcrecordid>eNqFkM9LwzAUx4MobsydPCu9Ccpc8pK0zUWQ-WPCwIN6Dmn66iJrO5NW8b-3o3PoyVzywvvwfXkfQo4ZvZSCwhQog6lIuaDJHhkCVXQipVL7uzqlAzIO4Y12R1KQiTwkAwARQ_cYEnnjQuNd1jaurqK6iGbG587Y6KnBMprhahUiV0XNEqN5W5oqmqPxzRE5KMwq4Hh7j8jL3e3zbD5ZPN4_zK4XEwspJBMjqI15msSQIy0yZnIGIDnPY7GpaSKzJEsUZGARuE0LwVOLKFmRKQRl-Yhc9bnrNisxt1g13qz02rvS-C9dG6f_diq31K_1h-acgey0jMjZNsDX7y2GRpcu2G4rU2HdBq1iLhSPGXTkRU9aX4fgsdhNYVRvVOuNat2r7ujT3x_bsT9iO-C8B5auys2n-yftpIexQ7AwO1gkking33z8jiE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>963493612</pqid></control><display><type>article</type><title>Distribution of Cardiac Stem Cells in the Human Heart</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Arsalan, Mani ; Woitek, Felix ; Adams, Volker ; Linke, Axel ; Barten, Markus J. ; Dhein, Stefan ; Walther, Thomas ; Mohr, Friedrich-Wilhelm ; Garbade, Jens</creator><contributor>Quaini, F.</contributor><creatorcontrib>Arsalan, Mani ; Woitek, Felix ; Adams, Volker ; Linke, Axel ; Barten, Markus J. ; Dhein, Stefan ; Walther, Thomas ; Mohr, Friedrich-Wilhelm ; Garbade, Jens ; Quaini, F.</creatorcontrib><description>Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, P=0.922). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, P=0.035). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.</description><identifier>ISSN: 2090-5580</identifier><identifier>ISSN: 2090-5599</identifier><identifier>EISSN: 2090-5599</identifier><identifier>DOI: 10.5402/2012/483407</identifier><identifier>PMID: 22462025</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><ispartof>ISRN Cardiology, 2012, Vol.2012 (2012), p.1-5</ispartof><rights>Copyright © 2012 Mani Arsalan et al.</rights><rights>Copyright © 2012 Mani Arsalan et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2827-a40c638762de0fb1ad122533d641ad1075b7b792b2ce23c8f438cee51fb9e29c3</citedby><cites>FETCH-LOGICAL-c2827-a40c638762de0fb1ad122533d641ad1075b7b792b2ce23c8f438cee51fb9e29c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312548/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312548/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22462025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Quaini, F.</contributor><creatorcontrib>Arsalan, Mani</creatorcontrib><creatorcontrib>Woitek, Felix</creatorcontrib><creatorcontrib>Adams, Volker</creatorcontrib><creatorcontrib>Linke, Axel</creatorcontrib><creatorcontrib>Barten, Markus J.</creatorcontrib><creatorcontrib>Dhein, Stefan</creatorcontrib><creatorcontrib>Walther, Thomas</creatorcontrib><creatorcontrib>Mohr, Friedrich-Wilhelm</creatorcontrib><creatorcontrib>Garbade, Jens</creatorcontrib><title>Distribution of Cardiac Stem Cells in the Human Heart</title><title>ISRN Cardiology</title><addtitle>ISRN Cardiol</addtitle><description>Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, P=0.922). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, P=0.035). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.</description><issn>2090-5580</issn><issn>2090-5599</issn><issn>2090-5599</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><recordid>eNqFkM9LwzAUx4MobsydPCu9Ccpc8pK0zUWQ-WPCwIN6Dmn66iJrO5NW8b-3o3PoyVzywvvwfXkfQo4ZvZSCwhQog6lIuaDJHhkCVXQipVL7uzqlAzIO4Y12R1KQiTwkAwARQ_cYEnnjQuNd1jaurqK6iGbG587Y6KnBMprhahUiV0XNEqN5W5oqmqPxzRE5KMwq4Hh7j8jL3e3zbD5ZPN4_zK4XEwspJBMjqI15msSQIy0yZnIGIDnPY7GpaSKzJEsUZGARuE0LwVOLKFmRKQRl-Yhc9bnrNisxt1g13qz02rvS-C9dG6f_diq31K_1h-acgey0jMjZNsDX7y2GRpcu2G4rU2HdBq1iLhSPGXTkRU9aX4fgsdhNYVRvVOuNat2r7ujT3x_bsT9iO-C8B5auys2n-yftpIexQ7AwO1gkking33z8jiE</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Arsalan, Mani</creator><creator>Woitek, Felix</creator><creator>Adams, Volker</creator><creator>Linke, Axel</creator><creator>Barten, Markus J.</creator><creator>Dhein, Stefan</creator><creator>Walther, Thomas</creator><creator>Mohr, Friedrich-Wilhelm</creator><creator>Garbade, Jens</creator><general>Hindawi Puplishing Corporation</general><general>International Scholarly Research Network</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2012</creationdate><title>Distribution of Cardiac Stem Cells in the Human Heart</title><author>Arsalan, Mani ; Woitek, Felix ; Adams, Volker ; Linke, Axel ; Barten, Markus J. ; Dhein, Stefan ; Walther, Thomas ; Mohr, Friedrich-Wilhelm ; Garbade, Jens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2827-a40c638762de0fb1ad122533d641ad1075b7b792b2ce23c8f438cee51fb9e29c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arsalan, Mani</creatorcontrib><creatorcontrib>Woitek, Felix</creatorcontrib><creatorcontrib>Adams, Volker</creatorcontrib><creatorcontrib>Linke, Axel</creatorcontrib><creatorcontrib>Barten, Markus J.</creatorcontrib><creatorcontrib>Dhein, Stefan</creatorcontrib><creatorcontrib>Walther, Thomas</creatorcontrib><creatorcontrib>Mohr, Friedrich-Wilhelm</creatorcontrib><creatorcontrib>Garbade, Jens</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>ISRN Cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arsalan, Mani</au><au>Woitek, Felix</au><au>Adams, Volker</au><au>Linke, Axel</au><au>Barten, Markus J.</au><au>Dhein, Stefan</au><au>Walther, Thomas</au><au>Mohr, Friedrich-Wilhelm</au><au>Garbade, Jens</au><au>Quaini, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Cardiac Stem Cells in the Human Heart</atitle><jtitle>ISRN Cardiology</jtitle><addtitle>ISRN Cardiol</addtitle><date>2012</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>2090-5580</issn><issn>2090-5599</issn><eissn>2090-5599</eissn><abstract>Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, P=0.922). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, P=0.035). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>22462025</pmid><doi>10.5402/2012/483407</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2090-5580
ispartof ISRN Cardiology, 2012, Vol.2012 (2012), p.1-5
issn 2090-5580
2090-5599
2090-5599
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3312548
source EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access
title Distribution of Cardiac Stem Cells in the Human Heart
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A02%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution%20of%20Cardiac%20Stem%20Cells%20in%20the%20Human%20Heart&rft.jtitle=ISRN%20Cardiology&rft.au=Arsalan,%20Mani&rft.date=2012&rft.volume=2012&rft.issue=2012&rft.spage=1&rft.epage=5&rft.pages=1-5&rft.issn=2090-5580&rft.eissn=2090-5599&rft_id=info:doi/10.5402/2012/483407&rft_dat=%3Cproquest_pubme%3E963493612%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=963493612&rft_id=info:pmid/22462025&rfr_iscdi=true