Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

As technological platforms, approaches such as next-generation sequencing, microarray, and qRT-PCR have great promise for expanding our understanding of the breadth of molecular regulation. Newer approaches such as high-resolution RNA sequencing (RNA-Seq)1 provides new and expansive information abou...

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Veröffentlicht in:	Journal of Visualized Experiments 2011-11 (57)
Hauptverfasser:	Atkins, Norman, Miller, Charlie M., Owens, Joseph R., Turek, Fred W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - surgery Brain Chemistry Gene Expression Profiling - methods Mice Microdissection - methods Neuroscience RNA - chemistry RNA - genetics RNA - isolation & purification Sequence Analysis, RNA - methods
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