Bacteriophage T4 gene 27
Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate. This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the or...
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Veröffentlicht in: | Nucleic acids research 1990-05, Vol.18 (10), p.3046-3046 |
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creator | BOVA, R CASCINO, A CIPOLLARO, M GRAU, O MICHELI, M. R SANTORO, M STORLAZZI, A SCARLATO, V GARGANO, S |
description | Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate. This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the original clone 656. The sequence predicts a 391 amino acid residues protein with a MW 44,000 which is consistent with the reported MW of gene product 27. A number of T4 gene 27 amber mutants have been used in rescuing the wild type allele in infections of E. coli cells carrying appropriate subclones of the original clone. By this means the gene has been located between genes 51 and 28. |
doi_str_mv | 10.1093/nar/18.10.3046 |
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By this means the gene has been located between genes 51 and 28.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/18.10.3046</identifier><identifier>PMID: 2349100</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Bacteria ; base plate ; Base Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; genes ; Genes, Viral ; Genes. 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By this means the gene has been located between genes 51 and 28.</description><subject>Amino Acid Sequence</subject><subject>Bacteria</subject><subject>base plate</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes, Viral</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>proteins</subject><subject>T-Phages - genetics</subject><subject>Transcription, Genetic</subject><subject>Viral Proteins - genetics</subject><subject>Viral Structural Proteins - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtPAjEQbowGEb0aLyZc9LYwfbcHD0p8JSRe8NyU0sKaZRdbMPHfW8IG9cRlZprvMZ18CF1iGGDQdFjbOMQqzwMKTByhLqaCFEwLcoy6QIEXGJg6RWcpfQBghjnroA6hTGOALrp6sG7tY9msFnbu-xPWn_va94k8RyfBVslftL2H3p8eJ6OXYvz2_Dq6HxeOSbEuNBaaeBosqJnK9mAxmeaiCQdOGZ75IIkNwXpuWXBEgJwKrYgggXmQgfbQ3c53tZku_cz5eh1tZVaxXNr4bRpbmv9IXS7MvPkylIKiMutvW31sPjc-rc2yTM5Xla19s0lGagWgND9IJEBAcaEPEjEXVPG8vIcGO6KLTUrRh_2vMZhtOCaHY7DaPrfhZMH131v39DaNjN-0uE3OViHa2pXp11ULLRWh9AdGspTg</recordid><startdate>19900525</startdate><enddate>19900525</enddate><creator>BOVA, R</creator><creator>CASCINO, A</creator><creator>CIPOLLARO, M</creator><creator>GRAU, O</creator><creator>MICHELI, M. R</creator><creator>SANTORO, M</creator><creator>STORLAZZI, A</creator><creator>SCARLATO, V</creator><creator>GARGANO, S</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900525</creationdate><title>Bacteriophage T4 gene 27</title><author>BOVA, R ; CASCINO, A ; CIPOLLARO, M ; GRAU, O ; MICHELI, M. 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R</creatorcontrib><creatorcontrib>SANTORO, M</creatorcontrib><creatorcontrib>STORLAZZI, A</creatorcontrib><creatorcontrib>SCARLATO, V</creatorcontrib><creatorcontrib>GARGANO, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BOVA, R</au><au>CASCINO, A</au><au>CIPOLLARO, M</au><au>GRAU, O</au><au>MICHELI, M. R</au><au>SANTORO, M</au><au>STORLAZZI, A</au><au>SCARLATO, V</au><au>GARGANO, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacteriophage T4 gene 27</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1990-05-25</date><risdate>1990</risdate><volume>18</volume><issue>10</issue><spage>3046</spage><epage>3046</epage><pages>3046-3046</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate. This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the original clone 656. The sequence predicts a 391 amino acid residues protein with a MW 44,000 which is consistent with the reported MW of gene product 27. A number of T4 gene 27 amber mutants have been used in rescuing the wild type allele in infections of E. coli cells carrying appropriate subclones of the original clone. By this means the gene has been located between genes 51 and 28.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2349100</pmid><doi>10.1093/nar/18.10.3046</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Bacteria base plate Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology genes Genes, Viral Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data proteins T-Phages - genetics Transcription, Genetic Viral Proteins - genetics Viral Structural Proteins - genetics |
title | Bacteriophage T4 gene 27 |
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