Bacteriophage T4 gene 27

Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate. This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the or...

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Veröffentlicht in:Nucleic acids research 1990-05, Vol.18 (10), p.3046-3046
Hauptverfasser: BOVA, R, CASCINO, A, CIPOLLARO, M, GRAU, O, MICHELI, M. R, SANTORO, M, STORLAZZI, A, SCARLATO, V, GARGANO, S
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container_end_page 3046
container_issue 10
container_start_page 3046
container_title Nucleic acids research
container_volume 18
creator BOVA, R
CASCINO, A
CIPOLLARO, M
GRAU, O
MICHELI, M. R
SANTORO, M
STORLAZZI, A
SCARLATO, V
GARGANO, S
description Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate. This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the original clone 656. The sequence predicts a 391 amino acid residues protein with a MW 44,000 which is consistent with the reported MW of gene product 27. A number of T4 gene 27 amber mutants have been used in rescuing the wild type allele in infections of E. coli cells carrying appropriate subclones of the original clone. By this means the gene has been located between genes 51 and 28.
doi_str_mv 10.1093/nar/18.10.3046
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source Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central
subjects Amino Acid Sequence
Bacteria
base plate
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
genes
Genes, Viral
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
proteins
T-Phages - genetics
Transcription, Genetic
Viral Proteins - genetics
Viral Structural Proteins - genetics
title Bacteriophage T4 gene 27
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