Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium
Mast cells have been implicated to play a functional role in arthritis, especially in autoantibody-positive disease. Among the cytokines involved in rheumatoid arthritis (RA), IL-17 is an important inflammatory mediator. Recent data suggest that the synovial mast cell is a main producer of IL-17, al...
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| Veröffentlicht in: | Arthritis research & therapy 2011-01, Vol.13 (5), p.R150-R150 |
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| container_title | Arthritis research & therapy |
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| creator | Suurmond, Jolien Dorjée, Annemarie L Boon, Mariëtte R Knol, Edward F Huizinga, Tom W J Toes, René E M Schuerwegh, Annemie J M |
| description | Mast cells have been implicated to play a functional role in arthritis, especially in autoantibody-positive disease. Among the cytokines involved in rheumatoid arthritis (RA), IL-17 is an important inflammatory mediator. Recent data suggest that the synovial mast cell is a main producer of IL-17, although T cells have also been implicated as prominent IL-17 producers as well. We aimed to identify IL-17 expression by mast cells and T cells in synovium of arthritis patients.
Synovial samples of anticitrullinated protein antibody-positive (ACPA+) and ACPA-negative (ACPA-) RA and osteoarthritis (OA) patients were stained for IL-17 in combination with CD117 (mast cells), CD3 (T cells) and CD68 (macrophages). Concentrations of IL-17 in synovial fluid were determined by ELISA.
The number of IL-17+ cells in synovium was comparable in all groups. Although the vast majority of IL-17+ cells are mast cells, no difference in the percentage of IL-17+ mast cells was observed. Nonetheless, levels of IL-17 in synovial fluid were increased in ACPA+ RA patients compared to ACPA- RA and OA patients.
The synovial mast cell is the main IL-17+ cell in all three arthritis groups analyzed. These data are relevant for studies aimed at blocking IL-17 in the treatment of arthritis. |
| doi_str_mv | 10.1186/ar3466 |
| format | Article |
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Synovial samples of anticitrullinated protein antibody-positive (ACPA+) and ACPA-negative (ACPA-) RA and osteoarthritis (OA) patients were stained for IL-17 in combination with CD117 (mast cells), CD3 (T cells) and CD68 (macrophages). Concentrations of IL-17 in synovial fluid were determined by ELISA.
The number of IL-17+ cells in synovium was comparable in all groups. Although the vast majority of IL-17+ cells are mast cells, no difference in the percentage of IL-17+ mast cells was observed. Nonetheless, levels of IL-17 in synovial fluid were increased in ACPA+ RA patients compared to ACPA- RA and OA patients.
The synovial mast cell is the main IL-17+ cell in all three arthritis groups analyzed. These data are relevant for studies aimed at blocking IL-17 in the treatment of arthritis.</description><identifier>ISSN: 1478-6354</identifier><identifier>EISSN: 1478-6362</identifier><identifier>DOI: 10.1186/ar3466</identifier><identifier>PMID: 21933391</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Antibodies, Antinuclear - metabolism ; Arthritis, Rheumatoid - metabolism ; Arthritis, Rheumatoid - pathology ; Autoantibodies - metabolism ; Care and treatment ; Diagnosis ; Enzyme-linked immunosorbent assay ; Humans ; Immune system ; Interleukin-17 - biosynthesis ; Mast Cells - metabolism ; Mast Cells - pathology ; Osteoarthritis ; Osteoarthritis - metabolism ; Osteoarthritis - pathology ; Rheumatoid arthritis ; Risk factors ; Synovial Membrane - metabolism ; Synovial Membrane - pathology</subject><ispartof>Arthritis research & therapy, 2011-01, Vol.13 (5), p.R150-R150</ispartof><rights>COPYRIGHT 2011 BioMed Central Ltd.</rights><rights>Copyright ©2011 Suurmond et al.; licensee BioMed Central Ltd. 2011 Suurmond et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b485t-4407458b08e9f0598dc91a258ee4b2f7d469a3f08b05dfcfff43825d59afa6de3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308080/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308080/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21933391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suurmond, Jolien</creatorcontrib><creatorcontrib>Dorjée, Annemarie L</creatorcontrib><creatorcontrib>Boon, Mariëtte R</creatorcontrib><creatorcontrib>Knol, Edward F</creatorcontrib><creatorcontrib>Huizinga, Tom W J</creatorcontrib><creatorcontrib>Toes, René E M</creatorcontrib><creatorcontrib>Schuerwegh, Annemie J M</creatorcontrib><title>Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium</title><title>Arthritis research & therapy</title><addtitle>Arthritis Res Ther</addtitle><description>Mast cells have been implicated to play a functional role in arthritis, especially in autoantibody-positive disease. Among the cytokines involved in rheumatoid arthritis (RA), IL-17 is an important inflammatory mediator. Recent data suggest that the synovial mast cell is a main producer of IL-17, although T cells have also been implicated as prominent IL-17 producers as well. We aimed to identify IL-17 expression by mast cells and T cells in synovium of arthritis patients.
Synovial samples of anticitrullinated protein antibody-positive (ACPA+) and ACPA-negative (ACPA-) RA and osteoarthritis (OA) patients were stained for IL-17 in combination with CD117 (mast cells), CD3 (T cells) and CD68 (macrophages). Concentrations of IL-17 in synovial fluid were determined by ELISA.
The number of IL-17+ cells in synovium was comparable in all groups. Although the vast majority of IL-17+ cells are mast cells, no difference in the percentage of IL-17+ mast cells was observed. Nonetheless, levels of IL-17 in synovial fluid were increased in ACPA+ RA patients compared to ACPA- RA and OA patients.
The synovial mast cell is the main IL-17+ cell in all three arthritis groups analyzed. These data are relevant for studies aimed at blocking IL-17 in the treatment of arthritis.</description><subject>Antibodies, Antinuclear - metabolism</subject><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Arthritis, Rheumatoid - pathology</subject><subject>Autoantibodies - metabolism</subject><subject>Care and treatment</subject><subject>Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Humans</subject><subject>Immune system</subject><subject>Interleukin-17 - biosynthesis</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - metabolism</subject><subject>Osteoarthritis - pathology</subject><subject>Rheumatoid arthritis</subject><subject>Risk factors</subject><subject>Synovial Membrane - metabolism</subject><subject>Synovial Membrane - pathology</subject><issn>1478-6354</issn><issn>1478-6362</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kktv1DAQxy0EoqXAR0CROHBKiV9JfEGqKl5SERc4W5N4vGtI7MV2VtrP0y-Ku7u0rASeg8czv_mPX4S8pM0lpX37FiIXbfuInFPR9XXLW_b43pfijDxL6UfTMKaYeErOGFWcc0XPye0XSLkacZpSBRGrvMZqBucr5zPGCZefxaddvQnJZbfFI1qC4LMbXY7LNDkPGU21iSHjMTMEs3soAm-q2uMK9qu4xmWGHJwpLfM6FibtkZAyhodQ2vmwdcv8nDyxMCV8cZwvyPcP779df6pvvn78fH11Uw-il7kWoumE7IemR2UbqXozKgpM9ohiYLYzolXAbVMIaexorRW8Z9JIBRZag_yCvDvobpZhRjOizxEmvYluhrjTAZw-zXi31quw1Zw3fbEioA4Cgwv_ETjNjGHWh5crtW-OzWP4tWDKenbp7rLBY1iSVpKzpgxeyNcHcgUTaudtKFrjHa2vWMcp6-h-L5f_oIoZnN0YPFpX4icFr_4-_f2u__wV_hs6tsYD</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Suurmond, Jolien</creator><creator>Dorjée, Annemarie L</creator><creator>Boon, Mariëtte R</creator><creator>Knol, Edward F</creator><creator>Huizinga, Tom W J</creator><creator>Toes, René E M</creator><creator>Schuerwegh, Annemie J M</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium</title><author>Suurmond, Jolien ; Dorjée, Annemarie L ; Boon, Mariëtte R ; Knol, Edward F ; Huizinga, Tom W J ; Toes, René E M ; Schuerwegh, Annemie J M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b485t-4407458b08e9f0598dc91a258ee4b2f7d469a3f08b05dfcfff43825d59afa6de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibodies, Antinuclear - metabolism</topic><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Arthritis, Rheumatoid - pathology</topic><topic>Autoantibodies - metabolism</topic><topic>Care and treatment</topic><topic>Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Humans</topic><topic>Immune system</topic><topic>Interleukin-17 - biosynthesis</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - pathology</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis - metabolism</topic><topic>Osteoarthritis - pathology</topic><topic>Rheumatoid arthritis</topic><topic>Risk factors</topic><topic>Synovial Membrane - metabolism</topic><topic>Synovial Membrane - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suurmond, Jolien</creatorcontrib><creatorcontrib>Dorjée, Annemarie L</creatorcontrib><creatorcontrib>Boon, Mariëtte R</creatorcontrib><creatorcontrib>Knol, Edward F</creatorcontrib><creatorcontrib>Huizinga, Tom W J</creatorcontrib><creatorcontrib>Toes, René E M</creatorcontrib><creatorcontrib>Schuerwegh, Annemie J M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Arthritis research & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suurmond, Jolien</au><au>Dorjée, Annemarie L</au><au>Boon, Mariëtte R</au><au>Knol, Edward F</au><au>Huizinga, Tom W J</au><au>Toes, René E M</au><au>Schuerwegh, Annemie J M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium</atitle><jtitle>Arthritis research & therapy</jtitle><addtitle>Arthritis Res Ther</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>13</volume><issue>5</issue><spage>R150</spage><epage>R150</epage><pages>R150-R150</pages><issn>1478-6354</issn><eissn>1478-6362</eissn><abstract>Mast cells have been implicated to play a functional role in arthritis, especially in autoantibody-positive disease. Among the cytokines involved in rheumatoid arthritis (RA), IL-17 is an important inflammatory mediator. Recent data suggest that the synovial mast cell is a main producer of IL-17, although T cells have also been implicated as prominent IL-17 producers as well. We aimed to identify IL-17 expression by mast cells and T cells in synovium of arthritis patients.
Synovial samples of anticitrullinated protein antibody-positive (ACPA+) and ACPA-negative (ACPA-) RA and osteoarthritis (OA) patients were stained for IL-17 in combination with CD117 (mast cells), CD3 (T cells) and CD68 (macrophages). Concentrations of IL-17 in synovial fluid were determined by ELISA.
The number of IL-17+ cells in synovium was comparable in all groups. Although the vast majority of IL-17+ cells are mast cells, no difference in the percentage of IL-17+ mast cells was observed. Nonetheless, levels of IL-17 in synovial fluid were increased in ACPA+ RA patients compared to ACPA- RA and OA patients.
The synovial mast cell is the main IL-17+ cell in all three arthritis groups analyzed. These data are relevant for studies aimed at blocking IL-17 in the treatment of arthritis.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>21933391</pmid><doi>10.1186/ar3466</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Antibodies, Antinuclear - metabolism Arthritis, Rheumatoid - metabolism Arthritis, Rheumatoid - pathology Autoantibodies - metabolism Care and treatment Diagnosis Enzyme-linked immunosorbent assay Humans Immune system Interleukin-17 - biosynthesis Mast Cells - metabolism Mast Cells - pathology Osteoarthritis Osteoarthritis - metabolism Osteoarthritis - pathology Rheumatoid arthritis Risk factors Synovial Membrane - metabolism Synovial Membrane - pathology |
| title | Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium |
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