The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism
Sequence analysis of a substantial part of the polymerase gene of the murine coronavirus MHV-A59 revealed the 3' end of an open reading frame (ORF1a) overlapping with a large ORF (ORF1b; 2733 amino acids) which covers the 3' half of the polymerase gene. The expression of ORF1b occurs by a...
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| Veröffentlicht in: | Nucleic acids research 1990-04, Vol.18 (7), p.1825-1832 |
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| creator | Bredenbeek, P J Pachuk, C J Noten, A F Charité, J Luytjes, W Weiss, S R Spaan, W J |
| description | Sequence analysis of a substantial part of the polymerase gene of the murine coronavirus MHV-A59 revealed the 3' end of an open reading frame (ORF1a) overlapping with a large ORF (ORF1b; 2733 amino acids) which covers the 3' half of the polymerase gene. The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. Detailed analysis of the predicted amino acid sequence revealed sequence elements which are conserved in many DNA and RNA polymerases. |
| doi_str_mv | 10.1093/nar/18.7.1825 |
| format | Article |
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The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. 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The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. 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a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism</title><author>Bredenbeek, P J ; Pachuk, C J ; Noten, A F ; Charité, J ; Luytjes, W ; Weiss, S R ; Spaan, W J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-b9b50486782959bb39d925216dfeb8a59290ab6aa843102cbc70c211437b3ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Coronavirus</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Genes, Viral</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Murine coronavirus</topic><topic>Murine hepatitis virus - enzymology</topic><topic>Murine hepatitis virus - genetics</topic><topic>Nucleic Acid Conformation</topic><topic>Oligonucleotide Probes</topic><topic>Restriction Mapping</topic><topic>ribosomes</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>RNA-directed RNA polymerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bredenbeek, P J</creatorcontrib><creatorcontrib>Pachuk, C J</creatorcontrib><creatorcontrib>Noten, A F</creatorcontrib><creatorcontrib>Charité, J</creatorcontrib><creatorcontrib>Luytjes, W</creatorcontrib><creatorcontrib>Weiss, S R</creatorcontrib><creatorcontrib>Spaan, W J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bredenbeek, P J</au><au>Pachuk, C J</au><au>Noten, A F</au><au>Charité, J</au><au>Luytjes, W</au><au>Weiss, S R</au><au>Spaan, W J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; 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| ispartof | Nucleic acids research, 1990-04, Vol.18 (7), p.1825-1832 |
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| language | eng |
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| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Coronavirus DNA-Directed RNA Polymerases - genetics Gene Expression genes Genes, Viral Mice Molecular Sequence Data Murine coronavirus Murine hepatitis virus - enzymology Murine hepatitis virus - genetics Nucleic Acid Conformation Oligonucleotide Probes Restriction Mapping ribosomes Ribosomes - metabolism RNA, Viral - genetics RNA, Viral - isolation & purification RNA-directed RNA polymerase |
| title | The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism |
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