Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells
Background: We isolated tumour endothelial cells (TECs), demonstrated their abnormalities, compared gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and identified several genes upregulated in TECs. We focused on the gene encoding biglycan, a small leucine-...
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| Veröffentlicht in: | British journal of cancer 2012-03, Vol.106 (6), p.1214-1223 |
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| creator | Yamamoto, K Ohga, N Hida, Y Maishi, N Kawamoto, T Kitayama, K Akiyama, K Osawa, T Kondoh, M Matsuda, K Onodera, Y Fujie, M Kaga, K Hirano, S Shinohara, N Shindoh, M Hida, K |
| description | Background:
We isolated tumour endothelial cells (TECs), demonstrated their abnormalities, compared gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and identified several genes upregulated in TECs. We focused on the gene encoding biglycan, a small leucine-rich repeat proteoglycan. No report is available on biglycan expression or function in TECs.
Methods:
The NEC and TEC were isolated. We investigated the biglycan expression and function in TECs. Western blotting analysis of biglycan was performed on sera from cancer patients.
Results:
Biglycan expression levels were higher in TECs than in NECs. Biglycan knockdown inhibited cell migration and caused morphological changes in TECs. Furthermore, immunostaining revealed strong biglycan expression
in vivo
in human tumour vessels, as in mouse TECs. Biglycan was detected in the sera of cancer patients but was hardly detected in those of healthy volunteers.
Conclusion:
These findings suggested that biglycan is a novel TEC marker and a target for anti-angiogenic therapy. |
| doi_str_mv | 10.1038/bjc.2012.59 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3304426</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>928370342</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-def4668e26f2ee1d53459f5ff202f04b736dce039a4898b619606864548287953</originalsourceid><addsrcrecordid>eNptkc1rVDEUxYNY7FhduZcgiAv7xnx_bAQtWoWCG90JIZOXvGZ8k4zJe0L_--YxY6viIlwu98fJORwAnmG0xoiqN5utWxOEyZrrB2CFOSUdVkQ-BCuEkOyQJugUPK5121aNlHwETgmhkjHBV-D7-ziMN84mGCu0sO69iyE6uLPlhy_Qpr49aOcpuxKTb8sQ8-BTQ4J1Uy4wBzjNuzwX6FOfp2s_RjtC58exPgEnwY7VPz3OM_Dt44evF5-6qy-Xny_eXXWOSTZ1vQ9MCOWJCMR73HPKuA48BIJIQGwjqeidR1RbprTaCKwFEkowzhRRUnN6Bt4edPfzZucbm6ZiR7MvscW4MdlG8_clxWsz5F-GUsQYEU3g1VGg5J-zr5PZxbpEsMnnuRpNFJWIMtLIF_-Q2xY9tXQNkopjKha51wfIlVxr8eHOCkZm6cy0zszSmeG60c__dH_H_i6pAS-PgK3OjqHY5GK957gUUtNF6PzA1XZKgy_33v737y0ISa2o</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>927851366</pqid></control><display><type>article</type><title>Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells</title><source>MEDLINE</source><source>Nature</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Yamamoto, K ; Ohga, N ; Hida, Y ; Maishi, N ; Kawamoto, T ; Kitayama, K ; Akiyama, K ; Osawa, T ; Kondoh, M ; Matsuda, K ; Onodera, Y ; Fujie, M ; Kaga, K ; Hirano, S ; Shinohara, N ; Shindoh, M ; Hida, K</creator><creatorcontrib>Yamamoto, K ; Ohga, N ; Hida, Y ; Maishi, N ; Kawamoto, T ; Kitayama, K ; Akiyama, K ; Osawa, T ; Kondoh, M ; Matsuda, K ; Onodera, Y ; Fujie, M ; Kaga, K ; Hirano, S ; Shinohara, N ; Shindoh, M ; Hida, K</creatorcontrib><description>Background:
We isolated tumour endothelial cells (TECs), demonstrated their abnormalities, compared gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and identified several genes upregulated in TECs. We focused on the gene encoding biglycan, a small leucine-rich repeat proteoglycan. No report is available on biglycan expression or function in TECs.
Methods:
The NEC and TEC were isolated. We investigated the biglycan expression and function in TECs. Western blotting analysis of biglycan was performed on sera from cancer patients.
Results:
Biglycan expression levels were higher in TECs than in NECs. Biglycan knockdown inhibited cell migration and caused morphological changes in TECs. Furthermore, immunostaining revealed strong biglycan expression
in vivo
in human tumour vessels, as in mouse TECs. Biglycan was detected in the sera of cancer patients but was hardly detected in those of healthy volunteers.
Conclusion:
These findings suggested that biglycan is a novel TEC marker and a target for anti-angiogenic therapy.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.2012.59</identifier><identifier>PMID: 22374465</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/67/1059/602 ; 631/67/1857 ; 631/67/2328 ; Angiogenesis ; Animals ; Antibodies ; Antigens, CD - metabolism ; Autocrine Communication ; Biglycan - blood ; Biglycan - genetics ; Biglycan - metabolism ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Blood vessels ; Cancer Research ; Carcinoma, Renal Cell - blood ; Carcinoma, Renal Cell - blood supply ; Carcinoma, Renal Cell - metabolism ; Carcinoma, Renal Cell - pathology ; Cell adhesion & migration ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Dentistry ; Drug Resistance ; Endothelial Cells - metabolism ; Endothelial Cells - physiology ; Endothelium, Vascular - metabolism ; Endothelium, Vascular - pathology ; Epidemiology ; Gene expression ; Gene Knockdown Techniques ; Humans ; Kidney Neoplasms - blood ; Kidney Neoplasms - blood supply ; Kidney Neoplasms - metabolism ; Kidney Neoplasms - pathology ; Medical research ; Medical sciences ; Medicine ; Melanoma ; Melanoma - blood supply ; Melanoma - metabolism ; Melanoma - pathology ; Mice ; Mice, Nude ; Molecular Diagnostics ; Molecular Medicine ; Neoplasm Transplantation ; Oncology ; Thoracic surgery ; Tumors</subject><ispartof>British journal of cancer, 2012-03, Vol.106 (6), p.1214-1223</ispartof><rights>The Author(s) 2012</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Mar 13, 2012</rights><rights>Copyright © 2012 Cancer Research UK 2012 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-def4668e26f2ee1d53459f5ff202f04b736dce039a4898b619606864548287953</citedby><cites>FETCH-LOGICAL-c474t-def4668e26f2ee1d53459f5ff202f04b736dce039a4898b619606864548287953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3304426/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3304426/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,41487,42556,51318,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25767939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22374465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamamoto, K</creatorcontrib><creatorcontrib>Ohga, N</creatorcontrib><creatorcontrib>Hida, Y</creatorcontrib><creatorcontrib>Maishi, N</creatorcontrib><creatorcontrib>Kawamoto, T</creatorcontrib><creatorcontrib>Kitayama, K</creatorcontrib><creatorcontrib>Akiyama, K</creatorcontrib><creatorcontrib>Osawa, T</creatorcontrib><creatorcontrib>Kondoh, M</creatorcontrib><creatorcontrib>Matsuda, K</creatorcontrib><creatorcontrib>Onodera, Y</creatorcontrib><creatorcontrib>Fujie, M</creatorcontrib><creatorcontrib>Kaga, K</creatorcontrib><creatorcontrib>Hirano, S</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Shindoh, M</creatorcontrib><creatorcontrib>Hida, K</creatorcontrib><title>Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Background:
We isolated tumour endothelial cells (TECs), demonstrated their abnormalities, compared gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and identified several genes upregulated in TECs. We focused on the gene encoding biglycan, a small leucine-rich repeat proteoglycan. No report is available on biglycan expression or function in TECs.
Methods:
The NEC and TEC were isolated. We investigated the biglycan expression and function in TECs. Western blotting analysis of biglycan was performed on sera from cancer patients.
Results:
Biglycan expression levels were higher in TECs than in NECs. Biglycan knockdown inhibited cell migration and caused morphological changes in TECs. Furthermore, immunostaining revealed strong biglycan expression
in vivo
in human tumour vessels, as in mouse TECs. Biglycan was detected in the sera of cancer patients but was hardly detected in those of healthy volunteers.
Conclusion:
These findings suggested that biglycan is a novel TEC marker and a target for anti-angiogenic therapy.</description><subject>631/67/1059/602</subject><subject>631/67/1857</subject><subject>631/67/2328</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens, CD - metabolism</subject><subject>Autocrine Communication</subject><subject>Biglycan - blood</subject><subject>Biglycan - genetics</subject><subject>Biglycan - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood vessels</subject><subject>Cancer Research</subject><subject>Carcinoma, Renal Cell - blood</subject><subject>Carcinoma, Renal Cell - blood supply</subject><subject>Carcinoma, Renal Cell - metabolism</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Cell adhesion & migration</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Dentistry</subject><subject>Drug Resistance</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelial Cells - physiology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium, Vascular - pathology</subject><subject>Epidemiology</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Kidney Neoplasms - blood</subject><subject>Kidney Neoplasms - blood supply</subject><subject>Kidney Neoplasms - metabolism</subject><subject>Kidney Neoplasms - pathology</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Melanoma</subject><subject>Melanoma - blood supply</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - pathology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Molecular Diagnostics</subject><subject>Molecular Medicine</subject><subject>Neoplasm Transplantation</subject><subject>Oncology</subject><subject>Thoracic surgery</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkc1rVDEUxYNY7FhduZcgiAv7xnx_bAQtWoWCG90JIZOXvGZ8k4zJe0L_--YxY6viIlwu98fJORwAnmG0xoiqN5utWxOEyZrrB2CFOSUdVkQ-BCuEkOyQJugUPK5121aNlHwETgmhkjHBV-D7-ziMN84mGCu0sO69iyE6uLPlhy_Qpr49aOcpuxKTb8sQ8-BTQ4J1Uy4wBzjNuzwX6FOfp2s_RjtC58exPgEnwY7VPz3OM_Dt44evF5-6qy-Xny_eXXWOSTZ1vQ9MCOWJCMR73HPKuA48BIJIQGwjqeidR1RbprTaCKwFEkowzhRRUnN6Bt4edPfzZucbm6ZiR7MvscW4MdlG8_clxWsz5F-GUsQYEU3g1VGg5J-zr5PZxbpEsMnnuRpNFJWIMtLIF_-Q2xY9tXQNkopjKha51wfIlVxr8eHOCkZm6cy0zszSmeG60c__dH_H_i6pAS-PgK3OjqHY5GK957gUUtNF6PzA1XZKgy_33v737y0ISa2o</recordid><startdate>20120313</startdate><enddate>20120313</enddate><creator>Yamamoto, K</creator><creator>Ohga, N</creator><creator>Hida, Y</creator><creator>Maishi, N</creator><creator>Kawamoto, T</creator><creator>Kitayama, K</creator><creator>Akiyama, K</creator><creator>Osawa, T</creator><creator>Kondoh, M</creator><creator>Matsuda, K</creator><creator>Onodera, Y</creator><creator>Fujie, M</creator><creator>Kaga, K</creator><creator>Hirano, S</creator><creator>Shinohara, N</creator><creator>Shindoh, M</creator><creator>Hida, K</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120313</creationdate><title>Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells</title><author>Yamamoto, K ; Ohga, N ; Hida, Y ; Maishi, N ; Kawamoto, T ; Kitayama, K ; Akiyama, K ; Osawa, T ; Kondoh, M ; Matsuda, K ; Onodera, Y ; Fujie, M ; Kaga, K ; Hirano, S ; Shinohara, N ; Shindoh, M ; Hida, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-def4668e26f2ee1d53459f5ff202f04b736dce039a4898b619606864548287953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>631/67/1059/602</topic><topic>631/67/1857</topic><topic>631/67/2328</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens, CD - metabolism</topic><topic>Autocrine Communication</topic><topic>Biglycan - blood</topic><topic>Biglycan - genetics</topic><topic>Biglycan - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood vessels</topic><topic>Cancer Research</topic><topic>Carcinoma, Renal Cell - blood</topic><topic>Carcinoma, Renal Cell - blood supply</topic><topic>Carcinoma, Renal Cell - metabolism</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Cell adhesion & migration</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Dentistry</topic><topic>Drug Resistance</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelial Cells - physiology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium, Vascular - pathology</topic><topic>Epidemiology</topic><topic>Gene expression</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Kidney Neoplasms - blood</topic><topic>Kidney Neoplasms - blood supply</topic><topic>Kidney Neoplasms - metabolism</topic><topic>Kidney Neoplasms - pathology</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Melanoma</topic><topic>Melanoma - blood supply</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - pathology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Molecular Diagnostics</topic><topic>Molecular Medicine</topic><topic>Neoplasm Transplantation</topic><topic>Oncology</topic><topic>Thoracic surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamamoto, K</creatorcontrib><creatorcontrib>Ohga, N</creatorcontrib><creatorcontrib>Hida, Y</creatorcontrib><creatorcontrib>Maishi, N</creatorcontrib><creatorcontrib>Kawamoto, T</creatorcontrib><creatorcontrib>Kitayama, K</creatorcontrib><creatorcontrib>Akiyama, K</creatorcontrib><creatorcontrib>Osawa, T</creatorcontrib><creatorcontrib>Kondoh, M</creatorcontrib><creatorcontrib>Matsuda, K</creatorcontrib><creatorcontrib>Onodera, Y</creatorcontrib><creatorcontrib>Fujie, M</creatorcontrib><creatorcontrib>Kaga, K</creatorcontrib><creatorcontrib>Hirano, S</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Shindoh, M</creatorcontrib><creatorcontrib>Hida, K</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamoto, K</au><au>Ohga, N</au><au>Hida, Y</au><au>Maishi, N</au><au>Kawamoto, T</au><au>Kitayama, K</au><au>Akiyama, K</au><au>Osawa, T</au><au>Kondoh, M</au><au>Matsuda, K</au><au>Onodera, Y</au><au>Fujie, M</au><au>Kaga, K</au><au>Hirano, S</au><au>Shinohara, N</au><au>Shindoh, M</au><au>Hida, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2012-03-13</date><risdate>2012</risdate><volume>106</volume><issue>6</issue><spage>1214</spage><epage>1223</epage><pages>1214-1223</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Background:
We isolated tumour endothelial cells (TECs), demonstrated their abnormalities, compared gene expression profiles of TECs and normal endothelial cells (NECs) by microarray analysis and identified several genes upregulated in TECs. We focused on the gene encoding biglycan, a small leucine-rich repeat proteoglycan. No report is available on biglycan expression or function in TECs.
Methods:
The NEC and TEC were isolated. We investigated the biglycan expression and function in TECs. Western blotting analysis of biglycan was performed on sera from cancer patients.
Results:
Biglycan expression levels were higher in TECs than in NECs. Biglycan knockdown inhibited cell migration and caused morphological changes in TECs. Furthermore, immunostaining revealed strong biglycan expression
in vivo
in human tumour vessels, as in mouse TECs. Biglycan was detected in the sera of cancer patients but was hardly detected in those of healthy volunteers.
Conclusion:
These findings suggested that biglycan is a novel TEC marker and a target for anti-angiogenic therapy.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>22374465</pmid><doi>10.1038/bjc.2012.59</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/67/1059/602 631/67/1857 631/67/2328 Angiogenesis Animals Antibodies Antigens, CD - metabolism Autocrine Communication Biglycan - blood Biglycan - genetics Biglycan - metabolism Biological and medical sciences Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biomedicine Blood vessels Cancer Research Carcinoma, Renal Cell - blood Carcinoma, Renal Cell - blood supply Carcinoma, Renal Cell - metabolism Carcinoma, Renal Cell - pathology Cell adhesion & migration Cell Line, Tumor Cell Movement Cell Proliferation Dentistry Drug Resistance Endothelial Cells - metabolism Endothelial Cells - physiology Endothelium, Vascular - metabolism Endothelium, Vascular - pathology Epidemiology Gene expression Gene Knockdown Techniques Humans Kidney Neoplasms - blood Kidney Neoplasms - blood supply Kidney Neoplasms - metabolism Kidney Neoplasms - pathology Medical research Medical sciences Medicine Melanoma Melanoma - blood supply Melanoma - metabolism Melanoma - pathology Mice Mice, Nude Molecular Diagnostics Molecular Medicine Neoplasm Transplantation Oncology Thoracic surgery Tumors |
| title | Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells |
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