Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish
Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is...
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Veröffentlicht in: | Developmental biology 2011-12, Vol.360 (2), p.276-285 |
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description | Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one in which multiple organs and cell types can be assessed simultaneously
in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish (
Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology.
► Feeding fluorescent fatty acids to live zebrafish visualizes lipid metabolism. ► Larvae accumulate the fluorescent analogs throughout their digestive organs. ► Different chain length fatty acid analogs are differentially metabolized. ► Analogs can function as vital dyes to visualize organ and cellular morphology. |
doi_str_mv | 10.1016/j.ydbio.2011.09.010 |
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in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish (
Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology.
► Feeding fluorescent fatty acids to live zebrafish visualizes lipid metabolism. ► Larvae accumulate the fluorescent analogs throughout their digestive organs. ► Different chain length fatty acid analogs are differentially metabolized. ► Analogs can function as vital dyes to visualize organ and cellular morphology.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2011.09.010</identifier><identifier>PMID: 21968100</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biological Transport ; BODIPY-FL ; Boron Compounds ; Danio rerio ; digestive system ; Digestive System - anatomy & histology ; Digestive System - metabolism ; egg yolk ; Egg Yolk - metabolism ; etiology ; Fatty acid ; fatty acid metabolism ; fatty acids ; Fatty Acids - metabolism ; Fluorescent Dyes ; Freshwater ; fuels ; Intestine ; Larva - anatomy & histology ; Larva - physiology ; larvae ; Lipid Metabolism ; Liposomes ; Liver ; metabolic diseases ; Metabolism ; Microscopy, Fluorescence ; mutants ; Palmitic Acids ; Zebrafish ; Zebrafish - anatomy & histology ; Zebrafish - physiology ; Zebrafish Proteins - metabolism</subject><ispartof>Developmental biology, 2011-12, Vol.360 (2), p.276-285</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><rights>2011 Elsevier Inc. All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c613t-16c86ea6f1f206d378e28809c23f63fd224ee2900e674c0b62fe2272b46f5fb83</citedby><cites>FETCH-LOGICAL-c613t-16c86ea6f1f206d378e28809c23f63fd224ee2900e674c0b62fe2272b46f5fb83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0012160611012541$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21968100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carten, Juliana Debrito</creatorcontrib><creatorcontrib>Bradford, Mary Katherine</creatorcontrib><creatorcontrib>Farber, Steven Arthur</creatorcontrib><title>Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one in which multiple organs and cell types can be assessed simultaneously
in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish (
Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology.
► Feeding fluorescent fatty acids to live zebrafish visualizes lipid metabolism. ► Larvae accumulate the fluorescent analogs throughout their digestive organs. ► Different chain length fatty acid analogs are differentially metabolized. ► Analogs can function as vital dyes to visualize organ and cellular morphology.</description><subject>Animals</subject><subject>Biological Transport</subject><subject>BODIPY-FL</subject><subject>Boron Compounds</subject><subject>Danio rerio</subject><subject>digestive system</subject><subject>Digestive System - anatomy & histology</subject><subject>Digestive System - metabolism</subject><subject>egg yolk</subject><subject>Egg Yolk - metabolism</subject><subject>etiology</subject><subject>Fatty acid</subject><subject>fatty acid metabolism</subject><subject>fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fluorescent Dyes</subject><subject>Freshwater</subject><subject>fuels</subject><subject>Intestine</subject><subject>Larva - anatomy & histology</subject><subject>Larva - physiology</subject><subject>larvae</subject><subject>Lipid Metabolism</subject><subject>Liposomes</subject><subject>Liver</subject><subject>metabolic diseases</subject><subject>Metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>mutants</subject><subject>Palmitic Acids</subject><subject>Zebrafish</subject><subject>Zebrafish - anatomy & histology</subject><subject>Zebrafish - physiology</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2P0zAQhiMEYsvCL0CC3ODSMrZT1zmwElrxJa3EARZxsxxnnE6V2MVOKnV_PS5ZVnBZTj74mUev53VRPGewYsDkm93q2DYUVhwYW0G9AgYPigWDer1cy-rHw2IBwPiSSZBnxZOUdgAglBKPizPOaqkYwKLw3ylNpqcb8l3ZUodppAOWIXbGl0OI-23oQ3csjW9LN3k7UvDllGbaOYzoRzJ96cw4ZspSWw44mib0lIaSfNmfdDfYROMobZ8Wj5zpEz67Pc-L6w_vv11-Wl59-fj58t3V0komxpzZKolGOuY4yFZsFHKloLZcOClcy3mFyGsAlJvKQiO5Q843vKmkW7tGifPiYvbup2bA1uaU0fR6H2kw8aiDIf3vjaet7sJBCwFis15nwatbQQw_p7wVPVCy2PfGY5iSrpkCCbWQ_yeh4nnfCjL5-l4yKwWDSvJNRsWM2hhSiujuojPQp_b1Tv9uX5_a11Dr3H6eevH3q-9m_tSdgZcz4EzQpouU9PXXbJD5ayjBxGlxb2cCczsHwqiTJfQWW4poR90GujfCL7l3zUw</recordid><startdate>20111215</startdate><enddate>20111215</enddate><creator>Carten, Juliana Debrito</creator><creator>Bradford, Mary Katherine</creator><creator>Farber, Steven Arthur</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20111215</creationdate><title>Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish</title><author>Carten, Juliana Debrito ; Bradford, Mary Katherine ; Farber, Steven Arthur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c613t-16c86ea6f1f206d378e28809c23f63fd224ee2900e674c0b62fe2272b46f5fb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological Transport</topic><topic>BODIPY-FL</topic><topic>Boron Compounds</topic><topic>Danio rerio</topic><topic>digestive system</topic><topic>Digestive System - anatomy & histology</topic><topic>Digestive System - metabolism</topic><topic>egg yolk</topic><topic>Egg Yolk - metabolism</topic><topic>etiology</topic><topic>Fatty acid</topic><topic>fatty acid metabolism</topic><topic>fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Fluorescent Dyes</topic><topic>Freshwater</topic><topic>fuels</topic><topic>Intestine</topic><topic>Larva - anatomy & histology</topic><topic>Larva - physiology</topic><topic>larvae</topic><topic>Lipid Metabolism</topic><topic>Liposomes</topic><topic>Liver</topic><topic>metabolic diseases</topic><topic>Metabolism</topic><topic>Microscopy, Fluorescence</topic><topic>mutants</topic><topic>Palmitic Acids</topic><topic>Zebrafish</topic><topic>Zebrafish - anatomy & histology</topic><topic>Zebrafish - physiology</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carten, Juliana Debrito</creatorcontrib><creatorcontrib>Bradford, Mary Katherine</creatorcontrib><creatorcontrib>Farber, Steven Arthur</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carten, Juliana Debrito</au><au>Bradford, Mary Katherine</au><au>Farber, Steven Arthur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2011-12-15</date><risdate>2011</risdate><volume>360</volume><issue>2</issue><spage>276</spage><epage>285</epage><pages>276-285</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one in which multiple organs and cell types can be assessed simultaneously
in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish (
Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology.
► Feeding fluorescent fatty acids to live zebrafish visualizes lipid metabolism. ► Larvae accumulate the fluorescent analogs throughout their digestive organs. ► Different chain length fatty acid analogs are differentially metabolized. ► Analogs can function as vital dyes to visualize organ and cellular morphology.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21968100</pmid><doi>10.1016/j.ydbio.2011.09.010</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological Transport BODIPY-FL Boron Compounds Danio rerio digestive system Digestive System - anatomy & histology Digestive System - metabolism egg yolk Egg Yolk - metabolism etiology Fatty acid fatty acid metabolism fatty acids Fatty Acids - metabolism Fluorescent Dyes Freshwater fuels Intestine Larva - anatomy & histology Larva - physiology larvae Lipid Metabolism Liposomes Liver metabolic diseases Metabolism Microscopy, Fluorescence mutants Palmitic Acids Zebrafish Zebrafish - anatomy & histology Zebrafish - physiology Zebrafish Proteins - metabolism |
title | Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish |
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