Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish

Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is...

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Veröffentlicht in:Developmental biology 2011-12, Vol.360 (2), p.276-285
Hauptverfasser: Carten, Juliana Debrito, Bradford, Mary Katherine, Farber, Steven Arthur
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creator Carten, Juliana Debrito
Bradford, Mary Katherine
Farber, Steven Arthur
description Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one in which multiple organs and cell types can be assessed simultaneously in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish ( Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology. ► Feeding fluorescent fatty acids to live zebrafish visualizes lipid metabolism. ► Larvae accumulate the fluorescent analogs throughout their digestive organs. ► Different chain length fatty acid analogs are differentially metabolized. ► Analogs can function as vital dyes to visualize organ and cellular morphology.
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histology</subject><subject>Larva - physiology</subject><subject>larvae</subject><subject>Lipid Metabolism</subject><subject>Liposomes</subject><subject>Liver</subject><subject>metabolic diseases</subject><subject>Metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>mutants</subject><subject>Palmitic Acids</subject><subject>Zebrafish</subject><subject>Zebrafish - anatomy &amp; histology</subject><subject>Zebrafish - physiology</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2P0zAQhiMEYsvCL0CC3ODSMrZT1zmwElrxJa3EARZxsxxnnE6V2MVOKnV_PS5ZVnBZTj74mUev53VRPGewYsDkm93q2DYUVhwYW0G9AgYPigWDer1cy-rHw2IBwPiSSZBnxZOUdgAglBKPizPOaqkYwKLw3ylNpqcb8l3ZUodppAOWIXbGl0OI-23oQ3csjW9LN3k7UvDllGbaOYzoRzJ96cw4ZspSWw44mib0lIaSfNmfdDfYROMobZ8Wj5zpEz67Pc-L6w_vv11-Wl59-fj58t3V0komxpzZKolGOuY4yFZsFHKloLZcOClcy3mFyGsAlJvKQiO5Q843vKmkW7tGifPiYvbup2bA1uaU0fR6H2kw8aiDIf3vjaet7sJBCwFis15nwatbQQw_p7wVPVCy2PfGY5iSrpkCCbWQ_yeh4nnfCjL5-l4yKwWDSvJNRsWM2hhSiujuojPQp_b1Tv9uX5_a11Dr3H6eevH3q-9m_tSdgZcz4EzQpouU9PXXbJD5ayjBxGlxb2cCczsHwqiTJfQWW4poR90GujfCL7l3zUw</recordid><startdate>20111215</startdate><enddate>20111215</enddate><creator>Carten, Juliana Debrito</creator><creator>Bradford, Mary Katherine</creator><creator>Farber, Steven Arthur</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20111215</creationdate><title>Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish</title><author>Carten, Juliana Debrito ; 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subjects Animals
Biological Transport
BODIPY-FL
Boron Compounds
Danio rerio
digestive system
Digestive System - anatomy & histology
Digestive System - metabolism
egg yolk
Egg Yolk - metabolism
etiology
Fatty acid
fatty acid metabolism
fatty acids
Fatty Acids - metabolism
Fluorescent Dyes
Freshwater
fuels
Intestine
Larva - anatomy & histology
Larva - physiology
larvae
Lipid Metabolism
Liposomes
Liver
metabolic diseases
Metabolism
Microscopy, Fluorescence
mutants
Palmitic Acids
Zebrafish
Zebrafish - anatomy & histology
Zebrafish - physiology
Zebrafish Proteins - metabolism
title Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish
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