Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas
The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study,...
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| creator | Pateras, Ioannis S. Evangelou, Konstantinos Tsimaratou, Katerina Liontos, Michalis Sakellariou, Stratigoula Barlogiannis, Theodoros Karakitsos, Petros Papalois, Apostolos E. Kotsinas, Athanassios Gorgoulis, Vassilis G. |
| description | The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P |
| doi_str_mv | 10.1155/2012/823949 |
| format | Article |
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The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.</description><identifier>ISSN: 1110-7243</identifier><identifier>EISSN: 1110-7251</identifier><identifier>DOI: 10.1155/2012/823949</identifier><identifier>PMID: 22496616</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Aged ; Case-Control Studies ; DNA, Viral - analysis ; Female ; Heart Atria - pathology ; Heart Atria - virology ; Heart Neoplasms - chemistry ; Heart Neoplasms - pathology ; Heart Neoplasms - virology ; Heart Ventricles - pathology ; Heart Ventricles - virology ; Herpes Simplex - pathology ; Herpes Simplex - virology ; Herpesvirus 1, Human - genetics ; Herpesvirus 1, Human - isolation & purification ; Herpesvirus 2, Human - genetics ; Herpesvirus 2, Human - isolation & purification ; Histocytochemistry ; Humans ; Male ; Middle Aged ; Myxoma - chemistry ; Myxoma - pathology ; Myxoma - virology ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Statistics, Nonparametric</subject><ispartof>Journal of biomedicine & biotechnology, 2012-01, Vol.2012 (2012), p.1-6</ispartof><rights>Copyright © 2012 Ioannis S. Pateras et al.</rights><rights>Copyright © 2012 Ioannis S. Pateras et al. 2012</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e298e491c60c5062079b6ae4a7b95b25c518bb5e67efeb0884984bb78114cd63</citedby><cites>FETCH-LOGICAL-c438t-e298e491c60c5062079b6ae4a7b95b25c518bb5e67efeb0884984bb78114cd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303684/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303684/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22496616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Eliopoulos, Aristides</contributor><creatorcontrib>Pateras, Ioannis S.</creatorcontrib><creatorcontrib>Evangelou, Konstantinos</creatorcontrib><creatorcontrib>Tsimaratou, Katerina</creatorcontrib><creatorcontrib>Liontos, Michalis</creatorcontrib><creatorcontrib>Sakellariou, Stratigoula</creatorcontrib><creatorcontrib>Barlogiannis, Theodoros</creatorcontrib><creatorcontrib>Karakitsos, Petros</creatorcontrib><creatorcontrib>Papalois, Apostolos E.</creatorcontrib><creatorcontrib>Kotsinas, Athanassios</creatorcontrib><creatorcontrib>Gorgoulis, Vassilis G.</creatorcontrib><title>Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas</title><title>Journal of biomedicine & biotechnology</title><addtitle>J Biomed Biotechnol</addtitle><description>The etiology of sporadic cardiac myxomas remains elusive. 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The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.</description><subject>Aged</subject><subject>Case-Control Studies</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Heart Atria - pathology</subject><subject>Heart Atria - virology</subject><subject>Heart Neoplasms - chemistry</subject><subject>Heart Neoplasms - pathology</subject><subject>Heart Neoplasms - virology</subject><subject>Heart Ventricles - pathology</subject><subject>Heart Ventricles - virology</subject><subject>Herpes Simplex - pathology</subject><subject>Herpes Simplex - virology</subject><subject>Herpesvirus 1, Human - genetics</subject><subject>Herpesvirus 1, Human - isolation & purification</subject><subject>Herpesvirus 2, Human - genetics</subject><subject>Herpesvirus 2, Human - isolation & purification</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myxoma - chemistry</subject><subject>Myxoma - pathology</subject><subject>Myxoma - virology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Statistics, Nonparametric</subject><issn>1110-7243</issn><issn>1110-7251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNqF0MtrFTEUBvAgin3oyrUyS6mMPSevSTaFcn1UqLiwuA1J5oyNzOOazNX2v3fq1IuuXJ3A-fHl8DH2DOE1olKnHJCfGi6stA_YISJC3XCFD_dvKQ7YUSnfALAx2j5mB5xLqzXqQ3b2hmaKc5rGauqqC8rbnm6qz2n4Pb-kvCs1Vn5sq5pXaaw2PrfJx-rj7c00-PKEPep8X-jp_TxmV-_eXm0u6stP7z9szi_rKIWZa-LWkLQYNUQFmkNjg_YkfROsClxFhSYERbqhjgIYI62RITQGUcZWi2N2tsZud2GgNtI4Z9-7bU6Dz7du8sn9uxnTtfs6_XBCgNBGLgEv7wPy9H1HZXZDKpH63o807YpDAJAghRQLfbXSmKdSMnX7bxDcXeHurnC3Fr7oF39ftrd_Gl7AyQqu09j6n-k_ac9XTAuhzu-xAgRE8QtP-pAR</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Pateras, Ioannis S.</creator><creator>Evangelou, Konstantinos</creator><creator>Tsimaratou, Katerina</creator><creator>Liontos, Michalis</creator><creator>Sakellariou, Stratigoula</creator><creator>Barlogiannis, Theodoros</creator><creator>Karakitsos, Petros</creator><creator>Papalois, Apostolos E.</creator><creator>Kotsinas, Athanassios</creator><creator>Gorgoulis, Vassilis G.</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas</title><author>Pateras, Ioannis S. ; 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The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>22496616</pmid><doi>10.1155/2012/823949</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Case-Control Studies DNA, Viral - analysis Female Heart Atria - pathology Heart Atria - virology Heart Neoplasms - chemistry Heart Neoplasms - pathology Heart Neoplasms - virology Heart Ventricles - pathology Heart Ventricles - virology Herpes Simplex - pathology Herpes Simplex - virology Herpesvirus 1, Human - genetics Herpesvirus 1, Human - isolation & purification Herpesvirus 2, Human - genetics Herpesvirus 2, Human - isolation & purification Histocytochemistry Humans Male Middle Aged Myxoma - chemistry Myxoma - pathology Myxoma - virology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Statistics, Nonparametric |
| title | Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
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