DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition
A region of DNA sequence of the bacterial transposon Tn7, which is required for transposition, has been determined. This DNA sequence completes an 8351 base pair (bp) region containing five long open reading frames (ORF's) that correspond to the genetically defined genes, tnsA, B, C, D and E, r...
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| Veröffentlicht in: | Nucleic acids research 1990-02, Vol.18 (4), p.901-911 |
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| creator | FLORES, C ISHTIAQ QADRI, M LICHTENSTEIN, C |
| description | A region of DNA sequence of the bacterial transposon Tn7, which is required for transposition, has been determined. This DNA sequence completes an 8351 base pair (bp) region containing five long open reading frames (ORF's) that correspond to the genetically defined genes, tnsA, B, C, D and E, required for Tn7 transposition. All of the ORF's are oriented in the same direction, ie. inward from the element's right end. The genes are in a very compact arrangement with the presumed initiation codons never more than two bases beyond the preceding termination codon. Domains with similarity to the helix-turn-helix genre of Cro-like, sequence specific DNA binding sites occur within the deduced amino acid (a.a.) sequence of the TnsA, TnsB, TnsD and TnsE proteins. Translation of the tnsC ORF reveals strong homology to a consensus sequence for nucleotide binding sites as well as a region of similarity to a transcriptional activator (MalT). No striking a.a. sequence similarity to other DNA recombinases is observed. The possible roles of these proteins in Tn7 transposition is discussed in light of the analysis presented. |
| doi_str_mv | 10.1093/nar/18.4.901 |
| format | Article |
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This DNA sequence completes an 8351 base pair (bp) region containing five long open reading frames (ORF's) that correspond to the genetically defined genes, tnsA, B, C, D and E, required for Tn7 transposition. All of the ORF's are oriented in the same direction, ie. inward from the element's right end. The genes are in a very compact arrangement with the presumed initiation codons never more than two bases beyond the preceding termination codon. Domains with similarity to the helix-turn-helix genre of Cro-like, sequence specific DNA binding sites occur within the deduced amino acid (a.a.) sequence of the TnsA, TnsB, TnsD and TnsE proteins. Translation of the tnsC ORF reveals strong homology to a consensus sequence for nucleotide binding sites as well as a region of similarity to a transcriptional activator (MalT). No striking a.a. sequence similarity to other DNA recombinases is observed. The possible roles of these proteins in Tn7 transposition is discussed in light of the analysis presented.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/18.4.901</identifier><identifier>PMID: 2156235</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Codon - genetics ; DNA Transposable Elements ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genic rearrangement. Recombination. Transposable element ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Restriction Mapping ; Sequence Homology, Nucleic Acid</subject><ispartof>Nucleic acids research, 1990-02, Vol.18 (4), p.901-911</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-2082655a15419730f05bc8a7aa8037f526af48bc7ce37f62deb774ba4716a01e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330344/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330344/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19518521$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2156235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FLORES, C</creatorcontrib><creatorcontrib>ISHTIAQ QADRI, M</creatorcontrib><creatorcontrib>LICHTENSTEIN, C</creatorcontrib><title>DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A region of DNA sequence of the bacterial transposon Tn7, which is required for transposition, has been determined. This DNA sequence completes an 8351 base pair (bp) region containing five long open reading frames (ORF's) that correspond to the genetically defined genes, tnsA, B, C, D and E, required for Tn7 transposition. All of the ORF's are oriented in the same direction, ie. inward from the element's right end. The genes are in a very compact arrangement with the presumed initiation codons never more than two bases beyond the preceding termination codon. Domains with similarity to the helix-turn-helix genre of Cro-like, sequence specific DNA binding sites occur within the deduced amino acid (a.a.) sequence of the TnsA, TnsB, TnsD and TnsE proteins. Translation of the tnsC ORF reveals strong homology to a consensus sequence for nucleotide binding sites as well as a region of similarity to a transcriptional activator (MalT). No striking a.a. sequence similarity to other DNA recombinases is observed. The possible roles of these proteins in Tn7 transposition is discussed in light of the analysis presented.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Codon - genetics</subject><subject>DNA Transposable Elements</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rVDEUxYModVrduRWy0dV709x8P8TFOG1VKLqp4C7kZZIaeZNMkzeF_vemdKi66iqE8zuXc-9B6A2QJZCBnSZbTkEv-XIg8AwtgEna80HS52hBGBE9EK5fouNafxMCHAQ_QkcUhKRMLNDPs28rXP3N3ifnsU12uqux4hxwiLceX_vk6wc8p7rq8KcOrzt81qgNPu9waa5Y_AaHXPBVUnguNtVdrnGOOb1CL4Kdqn99eE_Qj4vzq_WX_vL756_r1WXvGFdzT4mmUgjbYsGgGAlEjE5bZa0mTAVBpQ1cj045376SbvyoFB8tVyAtAc9O0MeHubv9uPUb51OLMZldiVtb7ky20fyvpPjLXOdbwxhhnDf_-4O_5HaFOpttrM5Pk00-76tRg1RCU3gSpAS0FvppsJ1eS4B7sHsAXcm1Fh8eUwMx99WaVq0Bbbhp1Tb87b-bPsKHLpv-7qDb6uwUWhsu1r8zBwFatEX-AM9iqjE</recordid><startdate>19900225</startdate><enddate>19900225</enddate><creator>FLORES, C</creator><creator>ISHTIAQ QADRI, M</creator><creator>LICHTENSTEIN, C</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900225</creationdate><title>DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition</title><author>FLORES, C ; ISHTIAQ QADRI, M ; LICHTENSTEIN, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-2082655a15419730f05bc8a7aa8037f526af48bc7ce37f62deb774ba4716a01e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Codon - genetics</topic><topic>DNA Transposable Elements</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FLORES, C</creatorcontrib><creatorcontrib>ISHTIAQ QADRI, M</creatorcontrib><creatorcontrib>LICHTENSTEIN, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FLORES, C</au><au>ISHTIAQ QADRI, M</au><au>LICHTENSTEIN, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1990-02-25</date><risdate>1990</risdate><volume>18</volume><issue>4</issue><spage>901</spage><epage>911</epage><pages>901-911</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A region of DNA sequence of the bacterial transposon Tn7, which is required for transposition, has been determined. This DNA sequence completes an 8351 base pair (bp) region containing five long open reading frames (ORF's) that correspond to the genetically defined genes, tnsA, B, C, D and E, required for Tn7 transposition. All of the ORF's are oriented in the same direction, ie. inward from the element's right end. The genes are in a very compact arrangement with the presumed initiation codons never more than two bases beyond the preceding termination codon. Domains with similarity to the helix-turn-helix genre of Cro-like, sequence specific DNA binding sites occur within the deduced amino acid (a.a.) sequence of the TnsA, TnsB, TnsD and TnsE proteins. Translation of the tnsC ORF reveals strong homology to a consensus sequence for nucleotide binding sites as well as a region of similarity to a transcriptional activator (MalT). No striking a.a. sequence similarity to other DNA recombinases is observed. The possible roles of these proteins in Tn7 transposition is discussed in light of the analysis presented.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2156235</pmid><doi>10.1093/nar/18.4.901</doi><tpages>11</tpages></addata></record> |
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| ispartof | Nucleic acids research, 1990-02, Vol.18 (4), p.901-911 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Archive; PubMed Central |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Codon - genetics DNA Transposable Elements Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genic rearrangement. Recombination. Transposable element Molecular and cellular biology Molecular genetics Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid |
| title | DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition |
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