primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis
The 3-phosphoglycerate kinase (PGK) gene from the industrially important dairy yeast Kluyveromyces lactis was isolated from a partial genomic library derived from wild-type strain CBS2359 using the Saccharomyces cerevisiae PGK gene as a hybridization probe. The entire gene is contained on a HindIII...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1990-01, Vol.18 (2), p.365-365 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 365 |
|---|---|
| container_issue | 2 |
| container_start_page | 365 |
| container_title | Nucleic acids research |
| container_volume | 18 |
| creator | Fournier, A Fleer, R Yeh, P Mayaux, J.F |
| description | The 3-phosphoglycerate kinase (PGK) gene from the industrially important dairy yeast Kluyveromyces lactis was isolated from a partial genomic library derived from wild-type strain CBS2359 using the Saccharomyces cerevisiae PGK gene as a hybridization probe. The entire gene is contained on a HindIII fragment which is 4 kb in size and able to support growth of a pgk null-mutant of S. cerevisiae on glucose when harbored on a 2 mu based multi-copy vector. We report here the complete nucleotide sequence of the K. lactis PGK structural gene which is identical in size (416 aa) and highly homologous (82% on the nucleotide level and 83% on the deduced amino acid level) to its S. cerevisiae counterpart. Compared to the PGK genes from humans, horse, Aspergillus nidulans , and Penicillium chrysogenum homologies on the amino acid levels are 65%, 65%, 66%, and 67%, respectively. |
| doi_str_mv | 10.1093/nar/18.2.365 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_330280</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16654161</sourcerecordid><originalsourceid>FETCH-LOGICAL-c493t-785759ab395816145e3df9ee0d1c2f7c8f9abebb983d1497bb36b5f6436a9ac3</originalsourceid><addsrcrecordid>eNqFkc2LFDEQxYMo6-zqzauYg4iCPZvKVycHD7Ksq-yCgiseQzpTmWnt6Z5NuhfmvzcyzaCnPRQpeL8q8uoR8gLYEpgV571P52CWfCm0ekQWIDSvpNX8MVkwwVQFTJqn5DTnX4yBBCVPyAkXXEPNFuTnLrVbn_Y0j2kK45SQDpGOG6Si2m2GXGrd7QMmPyL93fY-I3377er6HV1jjzSmYUuvu2l_j6UrXKadD2Obn5En0XcZn8_vGbn9dHl78bm6-Xr15eLjTRWkFWNVG1Ur6xthlQENUqFYRYvIVhB4rIOJRcSmsUasQNq6aYRuVNRSaG99EGfkw2Htbmq2uArYj8l3bjblBt-6_5W-3bj1cO-EYNywMv9mnk_D3YR5dNs2B-w63-MwZVfbmktTrvwQCLZcn1l4GNRayWK1gO8PYEhDzgnj8dfA3N9gXQnWgXHcldUFf_mv0yM8J1n017Puc_BdTL4PbT5i2iqulCzYqwMW_eD8OhXkx3fOQDCotQDLxB-BWbX_</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16654161</pqid></control><display><type>article</type><title>primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis</title><source>MEDLINE</source><source>Oxford Journals A-Z Collection</source><source>PubMed Central</source><creator>Fournier, A ; Fleer, R ; Yeh, P ; Mayaux, J.F</creator><creatorcontrib>Fournier, A ; Fleer, R ; Yeh, P ; Mayaux, J.F</creatorcontrib><description>The 3-phosphoglycerate kinase (PGK) gene from the industrially important dairy yeast Kluyveromyces lactis was isolated from a partial genomic library derived from wild-type strain CBS2359 using the Saccharomyces cerevisiae PGK gene as a hybridization probe. The entire gene is contained on a HindIII fragment which is 4 kb in size and able to support growth of a pgk null-mutant of S. cerevisiae on glucose when harbored on a 2 mu based multi-copy vector. We report here the complete nucleotide sequence of the K. lactis PGK structural gene which is identical in size (416 aa) and highly homologous (82% on the nucleotide level and 83% on the deduced amino acid level) to its S. cerevisiae counterpart. Compared to the PGK genes from humans, horse, Aspergillus nidulans , and Penicillium chrysogenum homologies on the amino acid levels are 65%, 65%, 66%, and 67%, respectively.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/18.2.365</identifier><identifier>PMID: 2326170</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Biological and medical sciences ; embl/x17654 ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Genes. Genome ; Kluyveromyces - enzymology ; Kluyveromyces - genetics ; Kluyveromyces lactis ; Kluyveromyces marxianus var. lactis ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequences ; phosphoglycerate kinase ; Phosphoglycerate Kinase - genetics ; Saccharomycetales - genetics ; structural genes</subject><ispartof>Nucleic acids research, 1990-01, Vol.18 (2), p.365-365</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-785759ab395816145e3df9ee0d1c2f7c8f9abebb983d1497bb36b5f6436a9ac3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330280/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330280/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6952554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2326170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fournier, A</creatorcontrib><creatorcontrib>Fleer, R</creatorcontrib><creatorcontrib>Yeh, P</creatorcontrib><creatorcontrib>Mayaux, J.F</creatorcontrib><title>primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The 3-phosphoglycerate kinase (PGK) gene from the industrially important dairy yeast Kluyveromyces lactis was isolated from a partial genomic library derived from wild-type strain CBS2359 using the Saccharomyces cerevisiae PGK gene as a hybridization probe. The entire gene is contained on a HindIII fragment which is 4 kb in size and able to support growth of a pgk null-mutant of S. cerevisiae on glucose when harbored on a 2 mu based multi-copy vector. We report here the complete nucleotide sequence of the K. lactis PGK structural gene which is identical in size (416 aa) and highly homologous (82% on the nucleotide level and 83% on the deduced amino acid level) to its S. cerevisiae counterpart. Compared to the PGK genes from humans, horse, Aspergillus nidulans , and Penicillium chrysogenum homologies on the amino acid levels are 65%, 65%, 66%, and 67%, respectively.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>embl/x17654</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Kluyveromyces - enzymology</subject><subject>Kluyveromyces - genetics</subject><subject>Kluyveromyces lactis</subject><subject>Kluyveromyces marxianus var. lactis</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>phosphoglycerate kinase</subject><subject>Phosphoglycerate Kinase - genetics</subject><subject>Saccharomycetales - genetics</subject><subject>structural genes</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo6-zqzauYg4iCPZvKVycHD7Ksq-yCgiseQzpTmWnt6Z5NuhfmvzcyzaCnPRQpeL8q8uoR8gLYEpgV571P52CWfCm0ekQWIDSvpNX8MVkwwVQFTJqn5DTnX4yBBCVPyAkXXEPNFuTnLrVbn_Y0j2kK45SQDpGOG6Si2m2GXGrd7QMmPyL93fY-I3377er6HV1jjzSmYUuvu2l_j6UrXKadD2Obn5En0XcZn8_vGbn9dHl78bm6-Xr15eLjTRWkFWNVG1Ur6xthlQENUqFYRYvIVhB4rIOJRcSmsUasQNq6aYRuVNRSaG99EGfkw2Htbmq2uArYj8l3bjblBt-6_5W-3bj1cO-EYNywMv9mnk_D3YR5dNs2B-w63-MwZVfbmktTrvwQCLZcn1l4GNRayWK1gO8PYEhDzgnj8dfA3N9gXQnWgXHcldUFf_mv0yM8J1n017Puc_BdTL4PbT5i2iqulCzYqwMW_eD8OhXkx3fOQDCotQDLxB-BWbX_</recordid><startdate>19900125</startdate><enddate>19900125</enddate><creator>Fournier, A</creator><creator>Fleer, R</creator><creator>Yeh, P</creator><creator>Mayaux, J.F</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900125</creationdate><title>primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis</title><author>Fournier, A ; Fleer, R ; Yeh, P ; Mayaux, J.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-785759ab395816145e3df9ee0d1c2f7c8f9abebb983d1497bb36b5f6436a9ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>embl/x17654</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Genes. Genome</topic><topic>Kluyveromyces - enzymology</topic><topic>Kluyveromyces - genetics</topic><topic>Kluyveromyces lactis</topic><topic>Kluyveromyces marxianus var. lactis</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>phosphoglycerate kinase</topic><topic>Phosphoglycerate Kinase - genetics</topic><topic>Saccharomycetales - genetics</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fournier, A</creatorcontrib><creatorcontrib>Fleer, R</creatorcontrib><creatorcontrib>Yeh, P</creatorcontrib><creatorcontrib>Mayaux, J.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fournier, A</au><au>Fleer, R</au><au>Yeh, P</au><au>Mayaux, J.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1990-01-25</date><risdate>1990</risdate><volume>18</volume><issue>2</issue><spage>365</spage><epage>365</epage><pages>365-365</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>The 3-phosphoglycerate kinase (PGK) gene from the industrially important dairy yeast Kluyveromyces lactis was isolated from a partial genomic library derived from wild-type strain CBS2359 using the Saccharomyces cerevisiae PGK gene as a hybridization probe. The entire gene is contained on a HindIII fragment which is 4 kb in size and able to support growth of a pgk null-mutant of S. cerevisiae on glucose when harbored on a 2 mu based multi-copy vector. We report here the complete nucleotide sequence of the K. lactis PGK structural gene which is identical in size (416 aa) and highly homologous (82% on the nucleotide level and 83% on the deduced amino acid level) to its S. cerevisiae counterpart. Compared to the PGK genes from humans, horse, Aspergillus nidulans , and Penicillium chrysogenum homologies on the amino acid levels are 65%, 65%, 66%, and 67%, respectively.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2326170</pmid><doi>10.1093/nar/18.2.365</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1990-01, Vol.18 (2), p.365-365 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_330280 |
| source | MEDLINE; Oxford Journals A-Z Collection; PubMed Central |
| subjects | Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences embl/x17654 Fundamental and applied biological sciences. Psychology Genes, Fungal Genes. Genome Kluyveromyces - enzymology Kluyveromyces - genetics Kluyveromyces lactis Kluyveromyces marxianus var. lactis Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences phosphoglycerate kinase Phosphoglycerate Kinase - genetics Saccharomycetales - genetics structural genes |
| title | primary structure of the 3-phosphoglycerate kinase (PGK) gene from Kluyveromyces lactis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T18%3A22%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=primary%20structure%20of%20the%203-phosphoglycerate%20kinase%20(PGK)%20gene%20from%20Kluyveromyces%20lactis&rft.jtitle=Nucleic%20acids%20research&rft.au=Fournier,%20A&rft.date=1990-01-25&rft.volume=18&rft.issue=2&rft.spage=365&rft.epage=365&rft.pages=365-365&rft.issn=0305-1048&rft.eissn=1362-4962&rft.coden=NARHAD&rft_id=info:doi/10.1093/nar/18.2.365&rft_dat=%3Cproquest_pubme%3E16654161%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16654161&rft_id=info:pmid/2326170&rfr_iscdi=true |