Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha
A cDNA library from the protist Euglena gracilis was used to isolate and sequence an ORF coding for the elongation factor protein EF-1 alpha. The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena...
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| Veröffentlicht in: | Nucleic acids research 1990-01, Vol.18 (1), p.75-82 |
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| creator | Montandon, P.E Stutz, E |
| description | A cDNA library from the protist Euglena gracilis was used to isolate and sequence an ORF coding for the elongation factor protein EF-1 alpha. The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena chloroplast EF-Tu. Homologous DNA probes interact with multiple fragments of Euglena nuclear restricted DNA typical for a multimembered gene family. We present the restriction sites map of four tef nuclear gene loci and postulate that the nuclear genome also contains tef related sequences (e.g. pseudogenes). Expression of tef gene(s) is monitored by Northern hybridization and the 5' end of a stable transcript (1.5 kb) is sequenced and shown to precede the start codon by 29 positions only. The steady state concentration of the 1.5 kb mRNA is not influenced by switching cell growth conditions from dark to light (chloroplast development). |
| doi_str_mv | 10.1093/nar/18.1.75 |
| format | Article |
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The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena chloroplast EF-Tu. Homologous DNA probes interact with multiple fragments of Euglena nuclear restricted DNA typical for a multimembered gene family. We present the restriction sites map of four tef nuclear gene loci and postulate that the nuclear genome also contains tef related sequences (e.g. pseudogenes). Expression of tef gene(s) is monitored by Northern hybridization and the 5' end of a stable transcript (1.5 kb) is sequenced and shown to precede the start codon by 29 positions only. 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Genome ; light ; loci ; messenger RNA ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; multigene family ; multiple genes ; nuclei ; nucleotide sequences ; open reading frames ; Peptide Elongation Factor 1 ; Peptide Elongation Factors - genetics ; plant proteins ; pseudogenes ; Restriction Mapping ; tef gene ; translation</subject><ispartof>Nucleic acids research, 1990-01, Vol.18 (1), p.75-82</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-15eac5c0511cb5c00535718336a58a1cef54a5f28bdc0b0767f170ff8ae3d2cc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330205/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC330205/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6719884$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2106666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Montandon, P.E</creatorcontrib><creatorcontrib>Stutz, E</creatorcontrib><title>Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A cDNA library from the protist Euglena gracilis was used to isolate and sequence an ORF coding for the elongation factor protein EF-1 alpha. The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena chloroplast EF-Tu. Homologous DNA probes interact with multiple fragments of Euglena nuclear restricted DNA typical for a multimembered gene family. We present the restriction sites map of four tef nuclear gene loci and postulate that the nuclear genome also contains tef related sequences (e.g. pseudogenes). Expression of tef gene(s) is monitored by Northern hybridization and the 5' end of a stable transcript (1.5 kb) is sequenced and shown to precede the start codon by 29 positions only. The steady state concentration of the 1.5 kb mRNA is not influenced by switching cell growth conditions from dark to light (chloroplast development).</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cell Nucleus</subject><subject>Cloning, Molecular</subject><subject>dark</subject><subject>DNA - genetics</subject><subject>elongation factor EF-1 alpha</subject><subject>embl/x16890</subject><subject>Euglena gracilis</subject><subject>Euglena gracilis - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>light</subject><subject>loci</subject><subject>messenger RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>multigene family</subject><subject>multiple genes</subject><subject>nuclei</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>Peptide Elongation Factor 1</subject><subject>Peptide Elongation Factors - genetics</subject><subject>plant proteins</subject><subject>pseudogenes</subject><subject>Restriction Mapping</subject><subject>tef gene</subject><subject>translation</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EKiFw4ozwAXFBm3rW613voQdUpYBUiUPp2Zp47Y2RYwd7F9F_X4dEEZw6lxnpfeZLLyFvga2A9fwyYLoEuYJVJ56RBfC2rpq-rZ-TBeNMVMAa-ZK8yvknY9CAaC7IRQ2sLbEg892UZj3NyVAMAzV_9snk7GKg0dJpa-h6Hr0JSMeE2nmXaZi1N5joaIKhOg4ujNTG9BeeEobscTr0Gx_DeCwt6qkQ65sKKPr9Fl-TFxZ9Nm9OeUnub9Y_rr9Wt9-_fLv-fFvpRvCpAmFQC80EgN6UzAQXHUjOWxQSQRsrGhS2lptBsw3r2s5Cx6yVaPhQa82X5Oo4dz9vdmbQJpQLvdont8P0oCI69b8S3FaN8bfinNVl25J8PPWn-Gs2eVI7l7XxHoOJc1Zd34pWMvkkWLMaQPCnwWKPLF8eVn86gjrFnJOx56uBqYPtqtiuQCpQ3YF-9--jZ_bkc9E_nHTMGr0tPmmXz1jbQS9lU7D3R8xiVDimgtzf1Qw4g66FXkj-CN63wMw</recordid><startdate>19900111</startdate><enddate>19900111</enddate><creator>Montandon, P.E</creator><creator>Stutz, E</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900111</creationdate><title>Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha</title><author>Montandon, P.E ; Stutz, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-15eac5c0511cb5c00535718336a58a1cef54a5f28bdc0b0767f170ff8ae3d2cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cell Nucleus</topic><topic>Cloning, Molecular</topic><topic>dark</topic><topic>DNA - genetics</topic><topic>elongation factor EF-1 alpha</topic><topic>embl/x16890</topic><topic>Euglena gracilis</topic><topic>Euglena gracilis - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Genes. 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The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena chloroplast EF-Tu. Homologous DNA probes interact with multiple fragments of Euglena nuclear restricted DNA typical for a multimembered gene family. We present the restriction sites map of four tef nuclear gene loci and postulate that the nuclear genome also contains tef related sequences (e.g. pseudogenes). Expression of tef gene(s) is monitored by Northern hybridization and the 5' end of a stable transcript (1.5 kb) is sequenced and shown to precede the start codon by 29 positions only. The steady state concentration of the 1.5 kb mRNA is not influenced by switching cell growth conditions from dark to light (chloroplast development).</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2106666</pmid><doi>10.1093/nar/18.1.75</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | OUP_牛津大学出版社过刊（NSTL购买）; MEDLINE; PubMed Central |
| subjects | Amino Acid Sequence amino acid sequences Animals Biological and medical sciences Blotting, Southern Cell Nucleus Cloning, Molecular dark DNA - genetics elongation factor EF-1 alpha embl/x16890 Euglena gracilis Euglena gracilis - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes Genes. Genome light loci messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data multigene family multiple genes nuclei nucleotide sequences open reading frames Peptide Elongation Factor 1 Peptide Elongation Factors - genetics plant proteins pseudogenes Restriction Mapping tef gene translation |
| title | Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha |
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