Adenosine triphosphate turnover in humans: decreased degradation during relative hyperphosphatemia
The regulation of ATP metabolism by inorganic phosphate (Pi) was examined in five normal volunteers through measurements of ATP degradation during relative Pi depletion and repletion states. Relative Pi depletion was achieved through dietary restriction and phosphate binders, whereas a Pi-repleted s...
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| Veröffentlicht in: | The Journal of clinical investigation 1989-09, Vol.84 (3), p.990-995 |
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| container_title | The Journal of clinical investigation |
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| creator | JOHNSON, M. A TEKKANAT, K SCHMALTZ, S. P FOX, I. H |
| description | The regulation of ATP metabolism by inorganic phosphate (Pi) was examined in five normal volunteers through measurements of ATP degradation during relative Pi depletion and repletion states. Relative Pi depletion was achieved through dietary restriction and phosphate binders, whereas a Pi-repleted state was produced by oral Pi supplementation. ATP was radioactively labeled by the infusion of [8(14)C]adenine. Fructose infusion was used to produce rapid ATP degradation during Pi depletion and repletion states. Baseline measurements indicated a significant decrease of Pi levels during phosphate depletion and no change in serum or urinary purines. Serum values of Pi declined 20 to 26% within 15 min after fructose infusion in all states. Urine measurements of ATP degradation products showed an eightfold increase within 15 min after fructose infusion in both Pi-depleted and -supplemented states. Urinary radioactive ATP degradation products were fourfold higher and urinary purine specific activity was more than threefold higher during Pi depletion as compared with Pi repletion. Our data indicate that there is decreased ATP degradation to purine end products during a relative phosphate repletion state as compared to a relative phosphate depletion state. These data show that ATP metabolism can be altered through manipulation of the relative Pi state in humans. |
| doi_str_mv | 10.1172/JCI114263 |
| format | Article |
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A ; TEKKANAT, K ; SCHMALTZ, S. P ; FOX, I. H</creator><creatorcontrib>JOHNSON, M. A ; TEKKANAT, K ; SCHMALTZ, S. P ; FOX, I. H</creatorcontrib><description>The regulation of ATP metabolism by inorganic phosphate (Pi) was examined in five normal volunteers through measurements of ATP degradation during relative Pi depletion and repletion states. Relative Pi depletion was achieved through dietary restriction and phosphate binders, whereas a Pi-repleted state was produced by oral Pi supplementation. ATP was radioactively labeled by the infusion of [8(14)C]adenine. Fructose infusion was used to produce rapid ATP degradation during Pi depletion and repletion states. Baseline measurements indicated a significant decrease of Pi levels during phosphate depletion and no change in serum or urinary purines. Serum values of Pi declined 20 to 26% within 15 min after fructose infusion in all states. Urine measurements of ATP degradation products showed an eightfold increase within 15 min after fructose infusion in both Pi-depleted and -supplemented states. Urinary radioactive ATP degradation products were fourfold higher and urinary purine specific activity was more than threefold higher during Pi depletion as compared with Pi repletion. Our data indicate that there is decreased ATP degradation to purine end products during a relative phosphate repletion state as compared to a relative phosphate depletion state. These data show that ATP metabolism can be altered through manipulation of the relative Pi state in humans.</description><identifier>ISSN: 0021-9738</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/JCI114263</identifier><identifier>PMID: 2760223</identifier><identifier>CODEN: JCINAO</identifier><language>eng</language><publisher>Ann Arbor, MI: American Society for Clinical Investigation</publisher><subject>550501 - Metabolism- Tracer Techniques ; ADENINES ; Adenosine Triphosphate - metabolism ; Adult ; AMINES ; Analytical, structural and metabolic biochemistry ; ANIMALS ; ANTIMETABOLITES ; AROMATICS ; ATP ; AZAARENES ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; CARBOHYDRATES ; CARBON 14 COMPOUNDS ; Carbon Radioisotopes - urine ; DRUGS ; FRUCTOSE ; Fructose - administration & dosage ; Fundamental and applied biological sciences. Psychology ; HETEROCYCLIC COMPOUNDS ; HEXOSES ; Humans ; Infusions, Intravenous ; ISOTOPE APPLICATIONS ; KETONES ; KINETICS ; LABELLED COMPOUNDS ; Male ; MAMMALS ; MAN ; METABOLISM ; MONOSACCHARIDES ; Nucleic acids ; Nucleic bases, nucleotides ; NUCLEOTIDES ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; OXYGEN COMPOUNDS ; PHOSPHATES ; Phosphates - administration & dosage ; Phosphates - metabolism ; Phosphorus - blood ; PHOSPHORUS COMPOUNDS ; PRIMATES ; PURINES ; Purines - blood ; Purines - urine ; REACTION KINETICS ; SACCHARIDES ; TRACER TECHNIQUES ; VERTEBRATES</subject><ispartof>The Journal of clinical investigation, 1989-09, Vol.84 (3), p.990-995</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-ded70b78a4b1975bea55e781727c95dda535e40890c2cd126d4f7eb70d98f19f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC329746/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC329746/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6825465$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2760223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5672451$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>JOHNSON, M. A</creatorcontrib><creatorcontrib>TEKKANAT, K</creatorcontrib><creatorcontrib>SCHMALTZ, S. P</creatorcontrib><creatorcontrib>FOX, I. H</creatorcontrib><title>Adenosine triphosphate turnover in humans: decreased degradation during relative hyperphosphatemia</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>The regulation of ATP metabolism by inorganic phosphate (Pi) was examined in five normal volunteers through measurements of ATP degradation during relative Pi depletion and repletion states. Relative Pi depletion was achieved through dietary restriction and phosphate binders, whereas a Pi-repleted state was produced by oral Pi supplementation. ATP was radioactively labeled by the infusion of [8(14)C]adenine. Fructose infusion was used to produce rapid ATP degradation during Pi depletion and repletion states. Baseline measurements indicated a significant decrease of Pi levels during phosphate depletion and no change in serum or urinary purines. Serum values of Pi declined 20 to 26% within 15 min after fructose infusion in all states. Urine measurements of ATP degradation products showed an eightfold increase within 15 min after fructose infusion in both Pi-depleted and -supplemented states. Urinary radioactive ATP degradation products were fourfold higher and urinary purine specific activity was more than threefold higher during Pi depletion as compared with Pi repletion. Our data indicate that there is decreased ATP degradation to purine end products during a relative phosphate repletion state as compared to a relative phosphate depletion state. These data show that ATP metabolism can be altered through manipulation of the relative Pi state in humans.</description><subject>550501 - Metabolism- Tracer Techniques</subject><subject>ADENINES</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Adult</subject><subject>AMINES</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANIMALS</subject><subject>ANTIMETABOLITES</subject><subject>AROMATICS</subject><subject>ATP</subject><subject>AZAARENES</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>CARBOHYDRATES</subject><subject>CARBON 14 COMPOUNDS</subject><subject>Carbon Radioisotopes - urine</subject><subject>DRUGS</subject><subject>FRUCTOSE</subject><subject>Fructose - administration & dosage</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>HEXOSES</subject><subject>Humans</subject><subject>Infusions, Intravenous</subject><subject>ISOTOPE APPLICATIONS</subject><subject>KETONES</subject><subject>KINETICS</subject><subject>LABELLED COMPOUNDS</subject><subject>Male</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>METABOLISM</subject><subject>MONOSACCHARIDES</subject><subject>Nucleic acids</subject><subject>Nucleic bases, nucleotides</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>OXYGEN COMPOUNDS</subject><subject>PHOSPHATES</subject><subject>Phosphates - administration & dosage</subject><subject>Phosphates - metabolism</subject><subject>Phosphorus - blood</subject><subject>PHOSPHORUS COMPOUNDS</subject><subject>PRIMATES</subject><subject>PURINES</subject><subject>Purines - blood</subject><subject>Purines - urine</subject><subject>REACTION KINETICS</subject><subject>SACCHARIDES</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES</subject><issn>0021-9738</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU2L1TAUhoM4jNfRhT9AKCKCi2o-m2TAxXDR-WDAja5DmpzeRtqkJu2F-fdW7qXo6pzD-5wvXoTeEPyJEEk_P-zvCeG0Yc_QjgihakWZeo52GFNSa8nUC_SylF8YE84Fv0SXVDaYUrZD7Y2HmEqIUM05TH0qU2_ntVhyTEfIVYhVv4w2luvKg8tgC_g1O2Tr7RxSrPySQzxUGYa1PkLVP02Qt0FjsK_QRWeHAq_P8Qr9_Pb1x_6ufvx-e7-_eawdp2KuPXiJW6ksb4mWogUrBEi1viedFt5bwQRwrDR21HlCG887Ca3EXquO6I5doS-nudPSjuAdxDnbwUw5jDY_mWSD-V-JoTeHdDSMasmbtf_dqT-VOZjiwgyudylGcLMRjaRckBX6cF6S0-8FymzGUBwMg42QlmKkJoyJRq_gxxPociolQ7cdQrD5a5rZTFvZt_9evpFnl1b9_Vm3xdmhyza6UDasUVTwRrA_Mzmheg</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>JOHNSON, M. A</creator><creator>TEKKANAT, K</creator><creator>SCHMALTZ, S. P</creator><creator>FOX, I. H</creator><general>American Society for Clinical Investigation</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19890901</creationdate><title>Adenosine triphosphate turnover in humans: decreased degradation during relative hyperphosphatemia</title><author>JOHNSON, M. A ; TEKKANAT, K ; SCHMALTZ, S. P ; FOX, I. H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-ded70b78a4b1975bea55e781727c95dda535e40890c2cd126d4f7eb70d98f19f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>550501 - Metabolism- Tracer Techniques</topic><topic>ADENINES</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Adult</topic><topic>AMINES</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMALS</topic><topic>ANTIMETABOLITES</topic><topic>AROMATICS</topic><topic>ATP</topic><topic>AZAARENES</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>CARBOHYDRATES</topic><topic>CARBON 14 COMPOUNDS</topic><topic>Carbon Radioisotopes - urine</topic><topic>DRUGS</topic><topic>FRUCTOSE</topic><topic>Fructose - administration & dosage</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>HEXOSES</topic><topic>Humans</topic><topic>Infusions, Intravenous</topic><topic>ISOTOPE APPLICATIONS</topic><topic>KETONES</topic><topic>KINETICS</topic><topic>LABELLED COMPOUNDS</topic><topic>Male</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>METABOLISM</topic><topic>MONOSACCHARIDES</topic><topic>Nucleic acids</topic><topic>Nucleic bases, nucleotides</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>OXYGEN COMPOUNDS</topic><topic>PHOSPHATES</topic><topic>Phosphates - administration & dosage</topic><topic>Phosphates - metabolism</topic><topic>Phosphorus - blood</topic><topic>PHOSPHORUS COMPOUNDS</topic><topic>PRIMATES</topic><topic>PURINES</topic><topic>Purines - blood</topic><topic>Purines - urine</topic><topic>REACTION KINETICS</topic><topic>SACCHARIDES</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JOHNSON, M. A</creatorcontrib><creatorcontrib>TEKKANAT, K</creatorcontrib><creatorcontrib>SCHMALTZ, S. P</creatorcontrib><creatorcontrib>FOX, I. H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JOHNSON, M. A</au><au>TEKKANAT, K</au><au>SCHMALTZ, S. P</au><au>FOX, I. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adenosine triphosphate turnover in humans: decreased degradation during relative hyperphosphatemia</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>84</volume><issue>3</issue><spage>990</spage><epage>995</epage><pages>990-995</pages><issn>0021-9738</issn><eissn>1558-8238</eissn><coden>JCINAO</coden><abstract>The regulation of ATP metabolism by inorganic phosphate (Pi) was examined in five normal volunteers through measurements of ATP degradation during relative Pi depletion and repletion states. Relative Pi depletion was achieved through dietary restriction and phosphate binders, whereas a Pi-repleted state was produced by oral Pi supplementation. ATP was radioactively labeled by the infusion of [8(14)C]adenine. Fructose infusion was used to produce rapid ATP degradation during Pi depletion and repletion states. Baseline measurements indicated a significant decrease of Pi levels during phosphate depletion and no change in serum or urinary purines. Serum values of Pi declined 20 to 26% within 15 min after fructose infusion in all states. Urine measurements of ATP degradation products showed an eightfold increase within 15 min after fructose infusion in both Pi-depleted and -supplemented states. Urinary radioactive ATP degradation products were fourfold higher and urinary purine specific activity was more than threefold higher during Pi depletion as compared with Pi repletion. Our data indicate that there is decreased ATP degradation to purine end products during a relative phosphate repletion state as compared to a relative phosphate depletion state. These data show that ATP metabolism can be altered through manipulation of the relative Pi state in humans.</abstract><cop>Ann Arbor, MI</cop><pub>American Society for Clinical Investigation</pub><pmid>2760223</pmid><doi>10.1172/JCI114263</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 550501 - Metabolism- Tracer Techniques ADENINES Adenosine Triphosphate - metabolism Adult AMINES Analytical, structural and metabolic biochemistry ANIMALS ANTIMETABOLITES AROMATICS ATP AZAARENES BASIC BIOLOGICAL SCIENCES BIOCHEMICAL REACTION KINETICS Biological and medical sciences CARBOHYDRATES CARBON 14 COMPOUNDS Carbon Radioisotopes - urine DRUGS FRUCTOSE Fructose - administration & dosage Fundamental and applied biological sciences. Psychology HETEROCYCLIC COMPOUNDS HEXOSES Humans Infusions, Intravenous ISOTOPE APPLICATIONS KETONES KINETICS LABELLED COMPOUNDS Male MAMMALS MAN METABOLISM MONOSACCHARIDES Nucleic acids Nucleic bases, nucleotides NUCLEOTIDES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS OXYGEN COMPOUNDS PHOSPHATES Phosphates - administration & dosage Phosphates - metabolism Phosphorus - blood PHOSPHORUS COMPOUNDS PRIMATES PURINES Purines - blood Purines - urine REACTION KINETICS SACCHARIDES TRACER TECHNIQUES VERTEBRATES |
| title | Adenosine triphosphate turnover in humans: decreased degradation during relative hyperphosphatemia |
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