Differential Binding of Co(II) and Zn(II) to Metallo-β-Lactamase Bla2 from Bacillus anthracis

In an effort to probe the structure, mechanism, and biochemical properties of metallo-β-lactamase Bla2 from Bacillus anthracis, the enzyme was overexpressed, purified, and characterized. Metal analyses demonstrated that recombinant Bla2 tightly binds 1 equiv of Zn(II). Steady-state kinetic studies s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the American Chemical Society 2009-08, Vol.131 (30), p.10753-10762
Hauptverfasser:	Hawk, Megan J, Breece, Robert M, Hajdin, Christine E, Bender, Katherine M, Hu, Zhenxin, Costello, Alison L, Bennett, Brian, Tierney, David L, Crowder, Michael W
Format:	Artikel
Sprache:	eng
Schlagworte:	BACILLUS Bacillus anthracis - enzymology BASIC BIOLOGICAL SCIENCES beta-Lactamases - biosynthesis beta-Lactamases - metabolism Cobalt - metabolism ELECTRON SPIN RESONANCE ENZYMES GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE KINETICS METALS national synchrotron light source NUCLEAR MAGNETIC RESONANCE Protein Binding PROTEINS Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Spectrum Analysis Substrate Specificity Zinc - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!