More than half of yeast U1 snRNA is dispensable for growth
Yeast U1 snRNA (568 nucleotides) is 3.5-fold larger than its mammalian counterpart (164 nucleotides) and contains apparent sequence homology only at the 5' and 3' ends. We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast c...
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| Veröffentlicht in: | Nucleic acids research 1991-12, Vol.19 (23), p.6367-6372 |
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| creator | Siliciano, P.G Kivens, W.J Guthrie, C |
| description | Yeast U1 snRNA (568 nucleotides) is 3.5-fold larger than its mammalian counterpart (164 nucleotides) and contains apparent sequence homology only at the 5' and 3' ends. We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. The minimal essential sequences of yeast U1 can be drawn in a secondary structure which resembles metazoan U1 in four of seven structural domains. |
| doi_str_mv | 10.1093/nar/19.23.6367 |
| format | Article |
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We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. 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We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. The minimal essential sequences of yeast U1 can be drawn in a secondary structure which resembles metazoan U1 in four of seven structural domains.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>cell growth</subject><subject>deletions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Kinetics</subject><subject>Metazoa</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleic Acid Conformation</subject><subject>nucleotide sequences</subject><subject>Phenotype</subject><subject>protein secondary structure</subject><subject>RNA Splicing</subject><subject>RNA, Fungal - physiology</subject><subject>RNA, Small Nuclear - physiology</subject><subject>Saccharomyces cerevisiae</subject><subject>small nuclear RNA</subject><subject>structure</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Genes, Fungal</topic><topic>Kinetics</topic><topic>Metazoa</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleic Acid Conformation</topic><topic>nucleotide sequences</topic><topic>Phenotype</topic><topic>protein secondary structure</topic><topic>RNA Splicing</topic><topic>RNA, Fungal - physiology</topic><topic>RNA, Small Nuclear - physiology</topic><topic>Saccharomyces cerevisiae</topic><topic>small nuclear RNA</topic><topic>structure</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>yeasts</topic><topic>Yeasts - genetics</topic><topic>Yeasts - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siliciano, P.G</creatorcontrib><creatorcontrib>Kivens, W.J</creatorcontrib><creatorcontrib>Guthrie, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siliciano, P.G</au><au>Kivens, W.J</au><au>Guthrie, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>More than half of yeast U1 snRNA is dispensable for growth</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-12-11</date><risdate>1991</risdate><volume>19</volume><issue>23</issue><spage>6367</spage><epage>6372</epage><pages>6367-6372</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Yeast U1 snRNA (568 nucleotides) is 3.5-fold larger than its mammalian counterpart (164 nucleotides) and contains apparent sequence homology only at the 5' and 3' ends. We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. The minimal essential sequences of yeast U1 can be drawn in a secondary structure which resembles metazoan U1 in four of seven structural domains.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1754372</pmid><doi>10.1093/nar/19.23.6367</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Base Sequence Biological and medical sciences Blotting, Northern cell growth deletions Fundamental and applied biological sciences. Psychology Genes, Fungal Kinetics Metazoa Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Nucleic Acid Conformation nucleotide sequences Phenotype protein secondary structure RNA Splicing RNA, Fungal - physiology RNA, Small Nuclear - physiology Saccharomyces cerevisiae small nuclear RNA structure Transcription. Transcription factor. Splicing. Rna processing yeasts Yeasts - genetics Yeasts - growth & development |
| title | More than half of yeast U1 snRNA is dispensable for growth |
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