Essential roles of zebrafish bmp2a, fgf10, and fgf24 in the specification of the ventral pancreas
In vertebrates, pancreas and liver arise from bipotential progenitors located in the embryonic gut endoderm. Bone morphogenic protein (BMP) and fibroblast growth factor (FGF) signaling pathways have been shown to induce hepatic specification while repressing pancreatic fate. Here we show that BMP an...
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| Veröffentlicht in: | Molecular biology of the cell 2012-03, Vol.23 (5), p.945-954 |
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| creator | Naye, François Voz, Marianne L Detry, Nathalie Hammerschmidt, Matthias Peers, Bernard Manfroid, Isabelle |
| description | In vertebrates, pancreas and liver arise from bipotential progenitors located in the embryonic gut endoderm. Bone morphogenic protein (BMP) and fibroblast growth factor (FGF) signaling pathways have been shown to induce hepatic specification while repressing pancreatic fate. Here we show that BMP and FGF factors also play crucial function, at slightly later stages, in the specification of the ventral pancreas. By analyzing the pancreatic markers pdx1, ptf1a, and hlxb9la in different zebrafish models of BMP loss of function, we demonstrate that the BMP pathway is required between 20 and 24 h postfertilization to specify the ventral pancreatic bud. Knockdown experiments show that bmp2a, expressed in the lateral plate mesoderm at these stages, is essential for ventral pancreas specification. Bmp2a action is not restricted to the pancreatic domain and is also required for the proper expression of hepatic markers. By contrast, through the analysis of fgf10(-/-); fgf24(-/-) embryos, we reveal the specific role of these two FGF ligands in the induction of the ventral pancreas and in the repression of the hepatic fate. These mutants display ventral pancreas agenesis and ectopic masses of hepatocytes. Overall, these data highlight the dynamic role of BMP and FGF in the patterning of the hepatopancreatic region. |
| doi_str_mv | 10.1091/mbc.E11-08-0664 |
| format | Article |
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Bone morphogenic protein (BMP) and fibroblast growth factor (FGF) signaling pathways have been shown to induce hepatic specification while repressing pancreatic fate. Here we show that BMP and FGF factors also play crucial function, at slightly later stages, in the specification of the ventral pancreas. By analyzing the pancreatic markers pdx1, ptf1a, and hlxb9la in different zebrafish models of BMP loss of function, we demonstrate that the BMP pathway is required between 20 and 24 h postfertilization to specify the ventral pancreatic bud. Knockdown experiments show that bmp2a, expressed in the lateral plate mesoderm at these stages, is essential for ventral pancreas specification. Bmp2a action is not restricted to the pancreatic domain and is also required for the proper expression of hepatic markers. By contrast, through the analysis of fgf10(-/-); fgf24(-/-) embryos, we reveal the specific role of these two FGF ligands in the induction of the ventral pancreas and in the repression of the hepatic fate. These mutants display ventral pancreas agenesis and ectopic masses of hepatocytes. 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By contrast, through the analysis of fgf10(-/-); fgf24(-/-) embryos, we reveal the specific role of these two FGF ligands in the induction of the ventral pancreas and in the repression of the hepatic fate. These mutants display ventral pancreas agenesis and ectopic masses of hepatocytes. Overall, these data highlight the dynamic role of BMP and FGF in the patterning of the hepatopancreatic region.</description><subject>Animals</subject><subject>Bone Morphogenetic Protein 2 - genetics</subject><subject>Bone Morphogenetic Protein 2 - physiology</subject><subject>Danio rerio</subject><subject>Fibroblast Growth Factor 10 - genetics</subject><subject>Fibroblast Growth Factor 10 - physiology</subject><subject>Fibroblast Growth Factors - genetics</subject><subject>Fibroblast Growth Factors - physiology</subject><subject>Freshwater</subject><subject>Gene Knockdown Techniques</subject><subject>Life sciences</subject><subject>Liver - embryology</subject><subject>Pancreas - embryology</subject><subject>Sciences du vivant</subject><subject>Signal Transduction</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - physiology</subject><subject>Zoologie</subject><subject>Zoology</subject><issn>1059-1524</issn><issn>1939-4586</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxSMEoqVw5oZ8g0PTevwV-4KEquVDqsQFzpbjHe8aJXGwsyuVvx6nWyq4cPKT_XtvZjxN8xroFVAD12PvrzYALdUtVUo8ac7BcNMKqdXTqqk0LUgmzpoXpfygFIRQ3fPmjDFWuU6dN25TCk5LdAPJacBCUiC_sM8uxLIn_Tgzd0nCLgC9JG7arpIJEiey7JGUGX0M0bslpml1rpfHGpdr3Owmn9GVl82z4IaCrx7Oi-b7x823m8_t7ddPX24-3LZedGJppTPBC4Udg20wofdBihA6JY0R2gHVKqAA7DRI7gRznedaUBocF2JbaX7RvD_lzod-xK0_tWHnHEeX72xy0f77MsW93aWj5cxQJaEG8FPAEHGHNuU-2iO7N97rw7CzztseLWNK2_r_2pjqevtQNqefByyLHWPxOAxuwnQo1jBVO-4oq-S7_5LQSc0VcKoren1CfU6lZAyPYwBdC4Oti7cIYKm26-Kr483f0z_yfzbNfwP_jKmX</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Naye, François</creator><creator>Voz, Marianne L</creator><creator>Detry, Nathalie</creator><creator>Hammerschmidt, Matthias</creator><creator>Peers, Bernard</creator><creator>Manfroid, Isabelle</creator><general>American Society for Cell Biology</general><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>Q33</scope><scope>5PM</scope></search><sort><creationdate>20120301</creationdate><title>Essential roles of zebrafish bmp2a, fgf10, and fgf24 in the specification of the ventral pancreas</title><author>Naye, François ; Voz, Marianne L ; Detry, Nathalie ; Hammerschmidt, Matthias ; Peers, Bernard ; Manfroid, Isabelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-5a9fc46e721df9fbcf54ff7659948a1086fe41e78153a42a7c38400fa344dbcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bone Morphogenetic Protein 2 - genetics</topic><topic>Bone Morphogenetic Protein 2 - physiology</topic><topic>Danio rerio</topic><topic>Fibroblast Growth Factor 10 - genetics</topic><topic>Fibroblast Growth Factor 10 - physiology</topic><topic>Fibroblast Growth Factors - genetics</topic><topic>Fibroblast Growth Factors - physiology</topic><topic>Freshwater</topic><topic>Gene Knockdown Techniques</topic><topic>Life sciences</topic><topic>Liver - embryology</topic><topic>Pancreas - embryology</topic><topic>Sciences du vivant</topic><topic>Signal Transduction</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - physiology</topic><topic>Zoologie</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naye, François</creatorcontrib><creatorcontrib>Voz, Marianne L</creatorcontrib><creatorcontrib>Detry, Nathalie</creatorcontrib><creatorcontrib>Hammerschmidt, Matthias</creatorcontrib><creatorcontrib>Peers, Bernard</creatorcontrib><creatorcontrib>Manfroid, Isabelle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Université de Liège - Open Repository and Bibliography (ORBI)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naye, François</au><au>Voz, Marianne L</au><au>Detry, Nathalie</au><au>Hammerschmidt, Matthias</au><au>Peers, Bernard</au><au>Manfroid, Isabelle</au><au>Bronner-Fraser, Marianne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Essential roles of zebrafish bmp2a, fgf10, and fgf24 in the specification of the ventral pancreas</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>23</volume><issue>5</issue><spage>945</spage><epage>954</epage><pages>945-954</pages><issn>1059-1524</issn><issn>1939-4586</issn><eissn>1939-4586</eissn><abstract>In vertebrates, pancreas and liver arise from bipotential progenitors located in the embryonic gut endoderm. Bone morphogenic protein (BMP) and fibroblast growth factor (FGF) signaling pathways have been shown to induce hepatic specification while repressing pancreatic fate. Here we show that BMP and FGF factors also play crucial function, at slightly later stages, in the specification of the ventral pancreas. By analyzing the pancreatic markers pdx1, ptf1a, and hlxb9la in different zebrafish models of BMP loss of function, we demonstrate that the BMP pathway is required between 20 and 24 h postfertilization to specify the ventral pancreatic bud. Knockdown experiments show that bmp2a, expressed in the lateral plate mesoderm at these stages, is essential for ventral pancreas specification. Bmp2a action is not restricted to the pancreatic domain and is also required for the proper expression of hepatic markers. 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| subjects | Animals Bone Morphogenetic Protein 2 - genetics Bone Morphogenetic Protein 2 - physiology Danio rerio Fibroblast Growth Factor 10 - genetics Fibroblast Growth Factor 10 - physiology Fibroblast Growth Factors - genetics Fibroblast Growth Factors - physiology Freshwater Gene Knockdown Techniques Life sciences Liver - embryology Pancreas - embryology Sciences du vivant Signal Transduction Zebrafish - embryology Zebrafish - genetics Zebrafish Proteins - genetics Zebrafish Proteins - physiology Zoologie Zoology |
| title | Essential roles of zebrafish bmp2a, fgf10, and fgf24 in the specification of the ventral pancreas |
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