Electroporation of Haemophilus influenzae is effective for transformation of plasmid but not chromosomal DNA

Electroporation of plasmid and chromosomal DNAs were tested in Haemophilus influenzae because of an interest in introducing DNA into mutants that are deficient in competence for transformation. The initial experiments were designed to investigate and optimize conditions for electroporation of H. inf...

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Veröffentlicht in:	Nucleic acids research 1991-07, Vol.19 (13), p.3625-3628
Hauptverfasser:	MITCHELL, M. A, SKOWRONEK, K, KAUC, L, GOODGAL, S. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Ampicillin Resistance - genetics Base Sequence Biological and medical sciences Biotechnology Chromosomes, Bacterial Deoxyribonuclease I - metabolism DNA, Bacterial - genetics Electric Stimulation Fundamental and applied biological sciences. Psychology Genetic engineering Genetic Markers Genetic technics Haemophilus influenzae Haemophilus influenzae - genetics Methods. Procedures. Technologies Miscellaneous Molecular Sequence Data Mutation - genetics Plasmids - genetics Synthetic digonucleotides and genes. Sequencing Transformation, Genetic
Online-Zugang:	Volltext
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