Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells
Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no app...
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| Veröffentlicht in: | Experimental cell research 2011-10, Vol.317 (16), p.2384-2389 |
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| creator | Zhu, Xiaodong Wang, Junxia Moriguchi, Kazuki Liow, Lu Ting Ahmed, Sohail Kaverina, Irina Murata-Hori, Maki |
| description | Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no apparent effects on the duration of cytokinesis, while overexpression of constitutively active Cdc42 (CACdc42) caused cytokinesis failure in normal rat kidney epithelial cells. Cells depleted of Cdc42 displayed abnormal cell morphology and caused a failure of tight accumulation of actin and RhoA at the equator. In contrast, in cells overexpressing CACdc42, actin formed abnormal bundles and RhoA was largely eliminated from the equator. Our results suggest that accurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis. Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells. |
| doi_str_mv | 10.1016/j.yexcr.2011.06.019 |
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Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no apparent effects on the duration of cytokinesis, while overexpression of constitutively active Cdc42 (CACdc42) caused cytokinesis failure in normal rat kidney epithelial cells. Cells depleted of Cdc42 displayed abnormal cell morphology and caused a failure of tight accumulation of actin and RhoA at the equator. In contrast, in cells overexpressing CACdc42, actin formed abnormal bundles and RhoA was largely eliminated from the equator. Our results suggest that accurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis. Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2011.06.019</identifier><identifier>PMID: 21763307</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actin ; Actin Cytoskeleton - metabolism ; Actin Cytoskeleton - pathology ; Actins - genetics ; Actins - metabolism ; Adherent mammalian cells ; Animals ; Cdc42 ; cdc42 GTP-Binding Protein - deficiency ; cdc42 GTP-Binding Protein - genetics ; cdc42 GTP-Binding Protein - metabolism ; Cell adhesion & migration ; Cell Line ; Cell Shape ; Cellular biology ; Cytokines ; Cytokinesis ; Cytokinesis - physiology ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Gene expression ; Kidney - cytology ; Microscopy, Phase-Contrast ; Mitosis - physiology ; Rats ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; rhoA GTP-Binding Protein - metabolism ; RNA, Small Interfering - genetics ; Time-Lapse Imaging ; Transfection</subject><ispartof>Experimental cell research, 2011-10, Vol.317 (16), p.2384-2389</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-feed75aaa0e9e65c1d504c4ccb771990fee786d746a1cc57e35f838a796b3303</citedby><cites>FETCH-LOGICAL-c551t-feed75aaa0e9e65c1d504c4ccb771990fee786d746a1cc57e35f838a796b3303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2011.06.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21763307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Xiaodong</creatorcontrib><creatorcontrib>Wang, Junxia</creatorcontrib><creatorcontrib>Moriguchi, Kazuki</creatorcontrib><creatorcontrib>Liow, Lu Ting</creatorcontrib><creatorcontrib>Ahmed, Sohail</creatorcontrib><creatorcontrib>Kaverina, Irina</creatorcontrib><creatorcontrib>Murata-Hori, Maki</creatorcontrib><title>Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no apparent effects on the duration of cytokinesis, while overexpression of constitutively active Cdc42 (CACdc42) caused cytokinesis failure in normal rat kidney epithelial cells. Cells depleted of Cdc42 displayed abnormal cell morphology and caused a failure of tight accumulation of actin and RhoA at the equator. In contrast, in cells overexpressing CACdc42, actin formed abnormal bundles and RhoA was largely eliminated from the equator. Our results suggest that accurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis. Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells.</description><subject>Actin</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Actin Cytoskeleton - pathology</subject><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Adherent mammalian cells</subject><subject>Animals</subject><subject>Cdc42</subject><subject>cdc42 GTP-Binding Protein - deficiency</subject><subject>cdc42 GTP-Binding Protein - genetics</subject><subject>cdc42 GTP-Binding Protein - metabolism</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Cell Shape</subject><subject>Cellular biology</subject><subject>Cytokines</subject><subject>Cytokinesis</subject><subject>Cytokinesis - physiology</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Gene expression</subject><subject>Kidney - cytology</subject><subject>Microscopy, Phase-Contrast</subject><subject>Mitosis - physiology</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>rhoA GTP-Binding Protein - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>Time-Lapse Imaging</subject><subject>Transfection</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2O0zAUhS0EYsrAEyAhiw2rBDtxbGcB0qjiTxoJFrO3XPumdUnsju1U9Bl4adzJMAIWrLw43zm-9x6EXlJSU0L52319gh8m1g2htCa8JrR_hFaU9KRqWNM8RitCKKuYbMQFepbSnhAiJeVP0UVDBW9bIlbo57cYDhBxhO086uyCx2HAa2tYg7XJ7ujyCbtU9NvZRbB4CBFnt93lO9ljE7wBn-Pi1RnnHeAC61xAO0fnt9iccvjuPKQSVCza7iAWD570NOnRaY_XMI7pOXoy6DHBi_v3Et18_HCz_lxdf_30ZX11XZmuo7kaAKzotNYEeuCdobYjzDBjNkLQvidFF5JbwbimxnQC2m6QrdSi55uydHuJ3i-xh3kzgV2mH9UhuknHkwraqb8V73ZqG46qbWQ5XFsC3twHxHA7Q8pqcsmUDbSHMCclJZOsIw0v5Ot_yH2Yoy_LnSHGpGjPULtAJoaUIgwPo1Cizk2rvbprWp2bVoSr0nRxvfpziwfP72oL8G4BoJzy6CCqZByUsmzp0WRlg_vvB78AXV6_SQ</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Zhu, Xiaodong</creator><creator>Wang, Junxia</creator><creator>Moriguchi, Kazuki</creator><creator>Liow, Lu Ting</creator><creator>Ahmed, Sohail</creator><creator>Kaverina, Irina</creator><creator>Murata-Hori, Maki</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111001</creationdate><title>Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells</title><author>Zhu, Xiaodong ; Wang, Junxia ; Moriguchi, Kazuki ; Liow, Lu Ting ; Ahmed, Sohail ; Kaverina, Irina ; Murata-Hori, Maki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-feed75aaa0e9e65c1d504c4ccb771990fee786d746a1cc57e35f838a796b3303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Actin</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Actin Cytoskeleton - pathology</topic><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Adherent mammalian cells</topic><topic>Animals</topic><topic>Cdc42</topic><topic>cdc42 GTP-Binding Protein - deficiency</topic><topic>cdc42 GTP-Binding Protein - genetics</topic><topic>cdc42 GTP-Binding Protein - metabolism</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Cell Shape</topic><topic>Cellular biology</topic><topic>Cytokines</topic><topic>Cytokinesis</topic><topic>Cytokinesis - physiology</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Gene expression</topic><topic>Kidney - cytology</topic><topic>Microscopy, Phase-Contrast</topic><topic>Mitosis - physiology</topic><topic>Rats</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>rhoA GTP-Binding Protein - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>Time-Lapse Imaging</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Xiaodong</creatorcontrib><creatorcontrib>Wang, Junxia</creatorcontrib><creatorcontrib>Moriguchi, Kazuki</creatorcontrib><creatorcontrib>Liow, Lu Ting</creatorcontrib><creatorcontrib>Ahmed, Sohail</creatorcontrib><creatorcontrib>Kaverina, Irina</creatorcontrib><creatorcontrib>Murata-Hori, Maki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Xiaodong</au><au>Wang, Junxia</au><au>Moriguchi, Kazuki</au><au>Liow, Lu Ting</au><au>Ahmed, Sohail</au><au>Kaverina, Irina</au><au>Murata-Hori, Maki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>317</volume><issue>16</issue><spage>2384</spage><epage>2389</epage><pages>2384-2389</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Cytokinesis in mammalian cells requires actin assembly at the equatorial region. Although functions of RhoA in this process have been well established, additional mechanisms are likely involved. We have examined if Cdc42 is involved in actin assembly during cytokinesis. Depletion of Cdc42 had no apparent effects on the duration of cytokinesis, while overexpression of constitutively active Cdc42 (CACdc42) caused cytokinesis failure in normal rat kidney epithelial cells. Cells depleted of Cdc42 displayed abnormal cell morphology and caused a failure of tight accumulation of actin and RhoA at the equator. In contrast, in cells overexpressing CACdc42, actin formed abnormal bundles and RhoA was largely eliminated from the equator. Our results suggest that accurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis. Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21763307</pmid><doi>10.1016/j.yexcr.2011.06.019</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Actin Cytoskeleton - metabolism Actin Cytoskeleton - pathology Actins - genetics Actins - metabolism Adherent mammalian cells Animals Cdc42 cdc42 GTP-Binding Protein - deficiency cdc42 GTP-Binding Protein - genetics cdc42 GTP-Binding Protein - metabolism Cell adhesion & migration Cell Line Cell Shape Cellular biology Cytokines Cytokinesis Cytokinesis - physiology Epithelial Cells - cytology Epithelial Cells - metabolism Epithelial Cells - pathology Gene expression Kidney - cytology Microscopy, Phase-Contrast Mitosis - physiology Rats Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism rhoA GTP-Binding Protein - metabolism RNA, Small Interfering - genetics Time-Lapse Imaging Transfection |
| title | Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian Cells |
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