Inhibition of restriction enzyme cleavage of DNA modified with 7-deaza-dGTP
DNA templates containing G+C rich regions or stable secondary structures can potentially pose problems for polymerase chain reaction amplification. Incorporation of the structure destabilizing analogue, 7-deaza-dGTP into the polymerase chain reaction reaction allows successful amplification of such...
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| Veröffentlicht in: | Nucleic acids research 1991-05, Vol.19 (10), p.2791-2791 |
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| container_title | Nucleic acids research |
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| creator | GRIME, S. K MARTIN, R. L HOLAWAY, B. L |
| description | DNA templates containing G+C rich regions or stable secondary structures can potentially pose problems for polymerase chain reaction amplification. Incorporation of the structure destabilizing analogue, 7-deaza-dGTP into the polymerase chain reaction reaction allows successful amplification of such template sequences. It has been reported that when 7-deaza-dGTP is substituted for dGTP in a DNA-fragment, the cleavage rate by the restriction enzyme EcoRI is reduced. We find that EcoRI as well as other commonly used restriction enzymes will not cut DNA containing 7-deaza-dGTP when it is incorporated into polymerase chain reaction product. |
| doi_str_mv | 10.1093/nar/19.10.2791 |
| format | Article |
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We find that EcoRI as well as other commonly used restriction enzymes will not cut DNA containing 7-deaza-dGTP when it is incorporated into polymerase chain reaction product.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/19.10.2791</identifier><identifier>PMID: 1645867</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>7-deaza-dGTP ; Analytical, structural and metabolic biochemistry ; Base Sequence ; Biological and medical sciences ; Deoxyguanine Nucleotides - pharmacology ; DNA - metabolism ; DNA Restriction Enzymes - antagonists & inhibitors ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Hydrolases ; Molecular Sequence Data ; polymerase chain reaction</subject><ispartof>Nucleic acids research, 1991-05, Vol.19 (10), p.2791-2791</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-5d2c069975885e09e0e3480ec400c7b3b7239b9b4add928c197c2f048d7e22e03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC328214/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC328214/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19786290$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1645867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRIME, S. K</creatorcontrib><creatorcontrib>MARTIN, R. L</creatorcontrib><creatorcontrib>HOLAWAY, B. L</creatorcontrib><title>Inhibition of restriction enzyme cleavage of DNA modified with 7-deaza-dGTP</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>DNA templates containing G+C rich regions or stable secondary structures can potentially pose problems for polymerase chain reaction amplification. Incorporation of the structure destabilizing analogue, 7-deaza-dGTP into the polymerase chain reaction reaction allows successful amplification of such template sequences. It has been reported that when 7-deaza-dGTP is substituted for dGTP in a DNA-fragment, the cleavage rate by the restriction enzyme EcoRI is reduced. We find that EcoRI as well as other commonly used restriction enzymes will not cut DNA containing 7-deaza-dGTP when it is incorporated into polymerase chain reaction product.</description><subject>7-deaza-dGTP</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Deoxyguanine Nucleotides - pharmacology</subject><subject>DNA - metabolism</subject><subject>DNA Restriction Enzymes - antagonists & inhibitors</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Molecular Sequence Data</subject><subject>polymerase chain reaction</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PHDEQxa0oEbmQtHRI24Ruj_HHru2CAkECKChQkNry2rOco_0Aew8Ef3283CkkFZU9er83mqdHyB6FJQXNDwcbD6nO_yWTmr4jC8prVgpds_dkARyqkoJQH8mnlH4DUEErsUN2aC0qVcsF-XExrEITpjAOxdgWEdMUg3sZcXh-6rFwHdoHe4uzfPrzuOhHH9qAvngM06qQpUf7bEt_dnP9mXxobZfwy_bdJb--f7s5OS8vr84uTo4vSydkPZWVZw5qrWWlVIWgEZALBegEgJMNbyTjutGNsN5rphzV0rE2h_ASGUPgu-Ros_du3fToHQ5TtJ25i6G38cmMNpj_lSGszO34YDhTjIrsP9j643i_zolNH5LDrrMDjutkFOTLhOZvglTzWs0tvAlWmgvNZnC5AV0cU4rY_r2agpk3mdxn3juPc5_ZsP9v1ld8U2DWv251m5zt2mgHF9IrpqWqmQb-B56yqCk</recordid><startdate>19910525</startdate><enddate>19910525</enddate><creator>GRIME, S. K</creator><creator>MARTIN, R. L</creator><creator>HOLAWAY, B. L</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910525</creationdate><title>Inhibition of restriction enzyme cleavage of DNA modified with 7-deaza-dGTP</title><author>GRIME, S. K ; MARTIN, R. L ; HOLAWAY, B. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-5d2c069975885e09e0e3480ec400c7b3b7239b9b4add928c197c2f048d7e22e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>7-deaza-dGTP</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Deoxyguanine Nucleotides - pharmacology</topic><topic>DNA - metabolism</topic><topic>DNA Restriction Enzymes - antagonists & inhibitors</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolases</topic><topic>Molecular Sequence Data</topic><topic>polymerase chain reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRIME, S. K</creatorcontrib><creatorcontrib>MARTIN, R. L</creatorcontrib><creatorcontrib>HOLAWAY, B. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of restriction enzyme cleavage of DNA modified with 7-deaza-dGTP</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-05-25</date><risdate>1991</risdate><volume>19</volume><issue>10</issue><spage>2791</spage><epage>2791</epage><pages>2791-2791</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>DNA templates containing G+C rich regions or stable secondary structures can potentially pose problems for polymerase chain reaction amplification. Incorporation of the structure destabilizing analogue, 7-deaza-dGTP into the polymerase chain reaction reaction allows successful amplification of such template sequences. It has been reported that when 7-deaza-dGTP is substituted for dGTP in a DNA-fragment, the cleavage rate by the restriction enzyme EcoRI is reduced. 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| ispartof | Nucleic acids research, 1991-05, Vol.19 (10), p.2791-2791 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | 7-deaza-dGTP Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Deoxyguanine Nucleotides - pharmacology DNA - metabolism DNA Restriction Enzymes - antagonists & inhibitors Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Molecular Sequence Data polymerase chain reaction |
| title | Inhibition of restriction enzyme cleavage of DNA modified with 7-deaza-dGTP |
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