Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy
The mucosa of the gastrointestinal tract is a dynamic tissue composed of numerous cell types with complex cellular functions. Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional...
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| Veröffentlicht in: | Histochemistry and cell biology 2012-03, Vol.137 (3), p.269-278 |
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| creator | Klinger, Antje Orzekowsky-Schroeder, Regina von Smolinski, Dorthe Blessenohl, Maike Schueth, Anna Koop, Norbert Huettmann, Gereon Gebert, Andreas |
| description | The mucosa of the gastrointestinal tract is a dynamic tissue composed of numerous cell types with complex cellular functions. Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional imaging of the vital murine intestine in anaesthetized mice. Using intravital autofluorescence 2-photon (A2P) microscopy we studied the choreographed interactions of enterocytes, goblet cells, enteroendocrine cells and brush cells with other cellular constituents of the small intestinal mucosa over several hours at a subcellular resolution and in three dimensions. Vigorously moving lymphoid cells and their interaction with constituent parts of the lamina propria were examined and quantitatively analyzed. Nuclear and lectin staining permitted simultaneous characterization of autofluorescence and admitted dyes and yielded additional spectral information that is crucial to the interpretation of the complex intestinal mucosa. This novel intravital approach provides detailed insights into the physiology of the small intestine and especially opens a new window for investigating cellular dynamics under nearly physiological conditions. |
| doi_str_mv | 10.1007/s00418-011-0905-0 |
| format | Article |
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Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional imaging of the vital murine intestine in anaesthetized mice. Using intravital autofluorescence 2-photon (A2P) microscopy we studied the choreographed interactions of enterocytes, goblet cells, enteroendocrine cells and brush cells with other cellular constituents of the small intestinal mucosa over several hours at a subcellular resolution and in three dimensions. Vigorously moving lymphoid cells and their interaction with constituent parts of the lamina propria were examined and quantitatively analyzed. Nuclear and lectin staining permitted simultaneous characterization of autofluorescence and admitted dyes and yielded additional spectral information that is crucial to the interpretation of the complex intestinal mucosa. This novel intravital approach provides detailed insights into the physiology of the small intestine and especially opens a new window for investigating cellular dynamics under nearly physiological conditions.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-011-0905-0</identifier><identifier>PMID: 22227801</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Anesthesia ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell adhesion & migration ; Cell Biology ; Developmental Biology ; Enterocytes - physiology ; Enterocytes - ultrastructure ; Female ; Imaging, Three-Dimensional - instrumentation ; Imaging, Three-Dimensional - methods ; Intestinal Mucosa - cytology ; Intestinal Mucosa - physiology ; Intestine, Small - cytology ; Intestine, Small - physiology ; Mice ; Mice, Inbred BALB C ; Microscopy ; Microscopy, Confocal - instrumentation ; Microscopy, Confocal - methods ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence - instrumentation ; Microscopy, Fluorescence - methods ; Microvilli - physiology ; Microvilli - ultrastructure ; Morphology ; Mucous membrane ; Original Paper ; Small intestine</subject><ispartof>Histochemistry and cell biology, 2012-03, Vol.137 (3), p.269-278</ispartof><rights>The Author(s) 2012</rights><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-1e0fe386c6f3f67bf2a2a5bce5e15cbad895709beabbe36f6399a6428dcbe8083</citedby><cites>FETCH-LOGICAL-c468t-1e0fe386c6f3f67bf2a2a5bce5e15cbad895709beabbe36f6399a6428dcbe8083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00418-011-0905-0$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00418-011-0905-0$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22227801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klinger, Antje</creatorcontrib><creatorcontrib>Orzekowsky-Schroeder, Regina</creatorcontrib><creatorcontrib>von Smolinski, Dorthe</creatorcontrib><creatorcontrib>Blessenohl, Maike</creatorcontrib><creatorcontrib>Schueth, Anna</creatorcontrib><creatorcontrib>Koop, Norbert</creatorcontrib><creatorcontrib>Huettmann, Gereon</creatorcontrib><creatorcontrib>Gebert, Andreas</creatorcontrib><title>Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>The mucosa of the gastrointestinal tract is a dynamic tissue composed of numerous cell types with complex cellular functions. 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This novel intravital approach provides detailed insights into the physiology of the small intestine and especially opens a new window for investigating cellular dynamics under nearly physiological conditions.</description><subject>Anesthesia</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell adhesion & migration</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Enterocytes - physiology</subject><subject>Enterocytes - ultrastructure</subject><subject>Female</subject><subject>Imaging, Three-Dimensional - instrumentation</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - physiology</subject><subject>Intestine, Small - cytology</subject><subject>Intestine, Small - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microscopy</subject><subject>Microscopy, Confocal - 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Study of the vital intestinal mucosa has been hampered by lack of suitable model systems. We here present a novel animal model that enables highly resolved three-dimensional imaging of the vital murine intestine in anaesthetized mice. Using intravital autofluorescence 2-photon (A2P) microscopy we studied the choreographed interactions of enterocytes, goblet cells, enteroendocrine cells and brush cells with other cellular constituents of the small intestinal mucosa over several hours at a subcellular resolution and in three dimensions. Vigorously moving lymphoid cells and their interaction with constituent parts of the lamina propria were examined and quantitatively analyzed. Nuclear and lectin staining permitted simultaneous characterization of autofluorescence and admitted dyes and yielded additional spectral information that is crucial to the interpretation of the complex intestinal mucosa. 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| ispartof | Histochemistry and cell biology, 2012-03, Vol.137 (3), p.269-278 |
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| language | eng |
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| subjects | Anesthesia Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell adhesion & migration Cell Biology Developmental Biology Enterocytes - physiology Enterocytes - ultrastructure Female Imaging, Three-Dimensional - instrumentation Imaging, Three-Dimensional - methods Intestinal Mucosa - cytology Intestinal Mucosa - physiology Intestine, Small - cytology Intestine, Small - physiology Mice Mice, Inbred BALB C Microscopy Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Microscopy, Electron, Transmission Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microvilli - physiology Microvilli - ultrastructure Morphology Mucous membrane Original Paper Small intestine |
| title | Complex morphology and functional dynamics of vital murine intestinal mucosa revealed by autofluorescence 2-photon microscopy |
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