Strengthening relationships: amyloids create adhesion nanodomains in yeasts
Budding yeasts adhere to biotic or abiotic surfaces and aggregate to form biofilms, using wall-anchored glycoprotein adhesins. The process is paradoxical: adhesins often show weak binding to specific ligands, yet mediate remarkably strong adherence. Single-molecule atomic force microscopy (AFM), gen...
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| Veröffentlicht in: | Trends in microbiology (Regular ed.) 2012-02, Vol.20 (2), p.59-65 |
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| container_title | Trends in microbiology (Regular ed.) |
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| creator | Lipke, Peter N Garcia, Melissa C Alsteens, David Ramsook, Caleen B Klotz, Stephen A Dufrêne, Yves F |
| description | Budding yeasts adhere to biotic or abiotic surfaces and aggregate to form biofilms, using wall-anchored glycoprotein adhesins. The process is paradoxical: adhesins often show weak binding to specific ligands, yet mediate remarkably strong adherence. Single-molecule atomic force microscopy (AFM), genomics, biochemistry and cell biology have recently explained the puzzle, with Candida albicans Als adhesins as the paradigm. The strength of adhesion results partly from force-activated amyloid-like clustering of hundreds of adhesin molecules to form arrays of ordered multimeric binding sites. The various protein domains of eukaryotic adhesins cooperate to facilitate this fascinating new mechanism of activation. |
| doi_str_mv | 10.1016/j.tim.2011.10.002 |
| format | Article |
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All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c628t-6183b109ac0430effe495bb03d258c497a4af6b8d9e3bfc409e48ee04d34fcbc3</citedby><cites>FETCH-LOGICAL-c628t-6183b109ac0430effe495bb03d258c497a4af6b8d9e3bfc409e48ee04d34fcbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0966842X11001910$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22099004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lipke, Peter N</creatorcontrib><creatorcontrib>Garcia, Melissa C</creatorcontrib><creatorcontrib>Alsteens, David</creatorcontrib><creatorcontrib>Ramsook, Caleen B</creatorcontrib><creatorcontrib>Klotz, Stephen A</creatorcontrib><creatorcontrib>Dufrêne, Yves F</creatorcontrib><title>Strengthening relationships: amyloids create adhesion nanodomains in yeasts</title><title>Trends in microbiology (Regular ed.)</title><addtitle>Trends Microbiol</addtitle><description>Budding yeasts adhere to biotic or abiotic surfaces and aggregate to form biofilms, using wall-anchored glycoprotein adhesins. 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The various protein domains of eukaryotic adhesins cooperate to facilitate this fascinating new mechanism of activation.</description><subject>adhesins</subject><subject>Amino Acid Sequence</subject><subject>amyloid</subject><subject>Amyloid - chemistry</subject><subject>Amyloid - metabolism</subject><subject>atomic force microscopy</subject><subject>Binding Sites</subject><subject>biofilm</subject><subject>Biofilms - growth & development</subject><subject>Candida albicans</subject><subject>Candida albicans - physiology</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Adhesion Molecules - chemistry</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>cell biology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - metabolism</subject><subject>genomics</subject><subject>glycoproteins</subject><subject>Internal Medicine</subject><subject>ligands</subject><subject>Microscopy, Atomic Force</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides - metabolism</subject><subject>Protein Binding</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Protein Multimerization</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>yeasts</subject><issn>0966-842X</issn><issn>1878-4380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk-P0zAQxSMEYsvCB-ACucElZcZ2UgekldCKf2IlDmUlbpbjTFqXxC62u1K_PY66rIDDnix7fvM0z2-K4jnCEgGbN7tlstOSAWK-LwHYg2KBciUrwSU8LBbQNk0lBftxVjyJcQcAdc3qx8UZY9C2AGJRfF2nQG6TtuSs25SBRp2sd3Fr9_Ftqafj6G0fSxNIJyp1v6WYy6XTzvd-0tbF0rrySDqm-LR4NOgx0rPb87y4_vjh--Xn6urbpy-X768q0zCZqgYl7xBabUBwoGEg0dZdB7xntTSiXWmhh6aTfUu8G4yAloQkAtFzMZjO8PPi4qS7P3QT9YZcCnpU-2AnHY7Ka6v-rTi7VRt_ozhbyVqILPDqViD4XweKSU02GhpH7cgfomoZCtbgCjL5-l4SJXBEBD6L4gk1wccYaLgbCEHNcamdynGpOa75KceVe1787eSu408-GXh5Agbtld4EG9X1Ois0AMgQGc_EuxNB-cdvLAUVjSVnqLeBTFK9t_cOcPFftxmts0aPP-lIcecPweUoFarIFKj1vFHzQmXLgG32_RuPMcX0</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Lipke, Peter N</creator><creator>Garcia, Melissa C</creator><creator>Alsteens, David</creator><creator>Ramsook, Caleen B</creator><creator>Klotz, Stephen A</creator><creator>Dufrêne, Yves F</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120201</creationdate><title>Strengthening relationships: amyloids create adhesion nanodomains in yeasts</title><author>Lipke, Peter N ; 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| subjects | adhesins Amino Acid Sequence amyloid Amyloid - chemistry Amyloid - metabolism atomic force microscopy Binding Sites biofilm Biofilms - growth & development Candida albicans Candida albicans - physiology Cell Adhesion - physiology Cell Adhesion Molecules - chemistry Cell Adhesion Molecules - metabolism cell biology Fungal Proteins - chemistry Fungal Proteins - metabolism genomics glycoproteins Internal Medicine ligands Microscopy, Atomic Force Molecular Sequence Data Oligosaccharides - metabolism Protein Binding Protein Interaction Domains and Motifs Protein Multimerization Saccharomyces cerevisiae - physiology yeasts |
| title | Strengthening relationships: amyloids create adhesion nanodomains in yeasts |
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