Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration
Nuclear and polysomal polyadenylated RNA popalations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenera...
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| Veröffentlicht in: | Nucleic acids research 1979, Vol.6 (6), p.2193-2208 |
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| creator | Wilkes, P.R. Birnie, G.D. Paul, J. |
| description | Nuclear and polysomal polyadenylated RNA popalations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10–15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cINA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysanal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver. |
| doi_str_mv | 10.1093/nar/6.6.2193 |
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It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10–15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cINA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysanal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/6.6.2193</identifier><identifier>PMID: 461186</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Base Sequence ; Cell Nucleus - metabolism ; Kinetics ; Liver - metabolism ; Liver Regeneration ; Male ; Nucleic Acid Hybridization ; Poly A - metabolism ; Polyribosomes - metabolism ; Rats ; RNA - metabolism</subject><ispartof>Nucleic acids research, 1979, Vol.6 (6), p.2193-2208</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-487a820658bc1f788d6e46f1b64c7e71f2a7299fa6d15c52eb323f85965471e03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC327845/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC327845/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/461186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilkes, P.R.</creatorcontrib><creatorcontrib>Birnie, G.D.</creatorcontrib><creatorcontrib>Paul, J.</creatorcontrib><title>Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Nuclear and polysomal polyadenylated RNA popalations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10–15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cINA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysanal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Nucleus - metabolism</subject><subject>Kinetics</subject><subject>Liver - metabolism</subject><subject>Liver Regeneration</subject><subject>Male</subject><subject>Nucleic Acid Hybridization</subject><subject>Poly A - metabolism</subject><subject>Polyribosomes - metabolism</subject><subject>Rats</subject><subject>RNA - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1vEzEQtRBfaeHGkcOeOLGpvz8OHEoEtFIEEioSgoPleGdTg-NN7d2K_HscUkXlVM1hZvTeG83MQ-gVwXOCDTtLLp_JuZxTYtgjNCNM0pYbSR-jGWZYtARz_RydlPILY8KJ4M_QUy4J0XKGfi6uXVpDaUJq0uQjuNy41DXbIe7KsHHxX-U6SLvoRuiar5_PmwI3EyRfVd2UQ1o32Y1tDLeQmwxrSFD7MKQX6EnvYoGXd_kUffv44Wpx0S6_fLpcnC9bz7kcW66V0xRLoVee9ErrTgKXPVlJ7hUo0lOnqDG9kx0RXlBYMcp6LYwUXBHA7BS9O8zdTqsNdB7SmF202xw2Lu_s4IL9H0nh2q6HW8uo0lxU_Zs7fR7qYWW0m1A8xOgSDFOxiktpFKUPEuv_BSNiP_HtgejzUEqG_rgMwXbvma2eWVlj71mlv75_wJF8MKnC7QEOZYQ_R9Tl31YqpoS9-P7DaiP5e2OW9or9Be1iowc</recordid><startdate>1979</startdate><enddate>1979</enddate><creator>Wilkes, P.R.</creator><creator>Birnie, G.D.</creator><creator>Paul, J.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1979</creationdate><title>Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration</title><author>Wilkes, P.R. ; Birnie, G.D. ; Paul, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-487a820658bc1f788d6e46f1b64c7e71f2a7299fa6d15c52eb323f85965471e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Nucleus - metabolism</topic><topic>Kinetics</topic><topic>Liver - metabolism</topic><topic>Liver Regeneration</topic><topic>Male</topic><topic>Nucleic Acid Hybridization</topic><topic>Poly A - metabolism</topic><topic>Polyribosomes - metabolism</topic><topic>Rats</topic><topic>RNA - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilkes, P.R.</creatorcontrib><creatorcontrib>Birnie, G.D.</creatorcontrib><creatorcontrib>Paul, J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilkes, P.R.</au><au>Birnie, G.D.</au><au>Paul, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1979</date><risdate>1979</risdate><volume>6</volume><issue>6</issue><spage>2193</spage><epage>2208</epage><pages>2193-2208</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Nuclear and polysomal polyadenylated RNA popalations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10–15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cINA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysanal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>461186</pmid><doi>10.1093/nar/6.6.2193</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1979, Vol.6 (6), p.2193-2208 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_327845 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Base Sequence Cell Nucleus - metabolism Kinetics Liver - metabolism Liver Regeneration Male Nucleic Acid Hybridization Poly A - metabolism Polyribosomes - metabolism Rats RNA - metabolism |
| title | Changes in nuclear and polysomal polyadenylated RNA sequences during rat-liver regeneration |
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