Noncanonical Amino Acid Labeling in Vivo to Visualize and Affinity Purify Newly Synthesized Proteins in Larval Zebrafish

Protein expression in the nervous system undergoes regulated changes in response to changes in behavioral states, in particular long-term memory formation. Recently, methods have been developed (BONCAT and FUNCAT), which introduce noncanonical amino acids bearing small bio-orthogonal functional grou...

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Veröffentlicht in:ACS chemical neuroscience 2012-01, Vol.3 (1), p.40-49
Hauptverfasser: Hinz, Flora I, Dieterich, Daniela C, Tirrell, David A, Schuman, Erin M
Format: Artikel
Sprache:eng
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