Partitioning of zinc and copper within subnuclear nucleoprotein particles

Nuclei from frozen calf thymus suspended in buffer were analyzed for metal content prior to and after repeated washing. After three such extractions about 0.1 μg Zn/mg DNA and 0.025 μg Cu/mg DNA remained tightly associated with chromatin. This level of metal was essentially unchanged with subsequent...

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Veröffentlicht in:	Nucleic acids research 1981-11, Vol.9 (21), p.5811-5824
Hauptverfasser:	Bryan, Sara E., Vizard, Douglas L., Beary, David A., LaBiche, Ronald A., Hardy, Kenneth J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cell Nucleus - metabolism Chromatin - metabolism Copper - isolation & purification Copper - metabolism DNA - metabolism Kinetics Nucleoproteins - metabolism Thymus Gland - metabolism Zinc - isolation & purification Zinc - metabolism
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