Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 1981-08, Vol.9 (16), p.3907-3918
Hauptverfasser:	Dingermann, Theodor, Sharp, Stephen, Appel, Bernd, DeFranco, Donald, Mount, Stephen, Heiermann, Reinhard, Pongs, Olaf, Söll, Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell-Free System Cloning, Molecular Drosophila Drosophila - genetics In Vitro Techniques Molecular Biology RNA - genetics RNA Polymerase III - genetics RNA, Transfer - genetics Transcription, Genetic Xenopus laevis - genetics
Online-Zugang:	Volltext
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