Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.
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| Veröffentlicht in: | Nucleic acids research 1981-08, Vol.9 (16), p.3907-3918 |
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| container_end_page | 3918 |
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| container_issue | 16 |
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| container_title | Nucleic acids research |
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| creator | Dingermann, Theodor Sharp, Stephen Appel, Bernd DeFranco, Donald Mount, Stephen Heiermann, Reinhard Pongs, Olaf Söll, Dieter |
| description | We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. |
| doi_str_mv | 10.1093/nar/9.16.3907 |
| format | Article |
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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1981-08, Vol.9 (16), p.3907-3918 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_327404 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Animals Cell-Free System Cloning, Molecular Drosophila Drosophila - genetics In Vitro Techniques Molecular Biology RNA - genetics RNA Polymerase III - genetics RNA, Transfer - genetics Transcription, Genetic Xenopus laevis - genetics |
| title | Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract |
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