The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay

SPO11 is a key protein for promoting meiotic recombination, by generating chromatin locus- and timing-specific DNA double-strand breaks (DSBs). The DSB activity of SPO11 was shown by genetic analyses, but whether SPO11 exerts DSB-forming activity by itself is still an unanswered question. DSB format...

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Veröffentlicht in:	BMC molecular biology 2012-01, Vol.13 (1), p.1-1, Article 1
Hauptverfasser:	Shingu, Yoshinori, Tokai, Takeshi, Agawa, Yasuo, Toyota, Kentaro, Ahamed, Selina, Kawagishi-Kobayashi, Makiko, Komatsu, Akira, Mikawa, Tsutomu, Yamamoto, Masa-Toshi, Wakasa, Kyo, Shibata, Takehiko, Kusano, Kohji
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Anthers Arabidopsis Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological Assay Chromatin Chromosomes Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA damage DNA Topoisomerases - genetics DNA Topoisomerases - metabolism Drosophila Drosophila - growth & development Endodeoxyribonucleases - genetics Endodeoxyribonucleases - metabolism Genes Genetic analysis Genetic recombination Meiosis Molecular Sequence Data Oocytes - metabolism Oryza - metabolism Oryza sativa Physiological aspects Plant Proteins - genetics Plant Proteins - metabolism Pollen Proteins Recombination RNA, Messenger - metabolism Transgenes Yeast
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