Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library
A mouse DNA clone containing the constant part of the immunoglobulin gamma 2b heavy chain was isolated from a mouse gene library. The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p gamma (11)7) containing a cDNA insert of the h...
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| Veröffentlicht in: | Nucleic acids research 1980-02, Vol.8 (3), p.453-466 |
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| creator | Zakut, R Givol, D Mory, Y Y |
| description | A mouse DNA clone containing the constant part of the immunoglobulin gamma 2b heavy chain was isolated from a mouse gene library. The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p gamma (11)7) containing a cDNA insert of the heavy chain constant region of the plasmacytoma MPC-11 (1). The Charon 4A clone contains a 14 kb insert which is cleaved by EcoRI into a 6.8 kb and 7.2 kb fragments, of which only the 6.8 kb contains the sequence for gamma 2b heavy chain. Restriction analysis and partial sequence of the insert in p gamma (11) 7 enabled us to obtain three fragments corresponding to the 5' (amino acid 161-302) middle (amino acid 302-443) and 3' (mostly non coding 107 bp) regions of the constant region. Restriction analysis of the Charon 4A clone and hybridisation to these nick translated fragments revealed that the gamma 2b constant region gene contains about 1.5 kb and has three intervening sequences. |
| doi_str_mv | 10.1093/nar/8.3.453 |
| format | Article |
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The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p gamma (11)7) containing a cDNA insert of the heavy chain constant region of the plasmacytoma MPC-11 (1). The Charon 4A clone contains a 14 kb insert which is cleaved by EcoRI into a 6.8 kb and 7.2 kb fragments, of which only the 6.8 kb contains the sequence for gamma 2b heavy chain. Restriction analysis and partial sequence of the insert in p gamma (11) 7 enabled us to obtain three fragments corresponding to the 5' (amino acid 161-302) middle (amino acid 302-443) and 3' (mostly non coding 107 bp) regions of the constant region. Restriction analysis of the Charon 4A clone and hybridisation to these nick translated fragments revealed that the gamma 2b constant region gene contains about 1.5 kb and has three intervening sequences.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/8.3.453</identifier><identifier>PMID: 6255443</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Base Sequence ; Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Neoplasm - genetics ; Immunoglobulin Constant Regions - genetics ; Immunoglobulin gamma-Chains - genetics ; Immunoglobulin Heavy Chains - genetics ; Mice - embryology ; Nucleic Acid Hybridization ; Plasmids</subject><ispartof>Nucleic acids research, 1980-02, Vol.8 (3), p.453-466</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC327283/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC327283/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6255443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zakut, R</creatorcontrib><creatorcontrib>Givol, D</creatorcontrib><creatorcontrib>Mory, Y Y</creatorcontrib><title>Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A mouse DNA clone containing the constant part of the immunoglobulin gamma 2b heavy chain was isolated from a mouse gene library. The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p gamma (11)7) containing a cDNA insert of the heavy chain constant region of the plasmacytoma MPC-11 (1). The Charon 4A clone contains a 14 kb insert which is cleaved by EcoRI into a 6.8 kb and 7.2 kb fragments, of which only the 6.8 kb contains the sequence for gamma 2b heavy chain. Restriction analysis and partial sequence of the insert in p gamma (11) 7 enabled us to obtain three fragments corresponding to the 5' (amino acid 161-302) middle (amino acid 302-443) and 3' (mostly non coding 107 bp) regions of the constant region. Restriction analysis of the Charon 4A clone and hybridisation to these nick translated fragments revealed that the gamma 2b constant region gene contains about 1.5 kb and has three intervening sequences.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Neoplasm - genetics</subject><subject>Immunoglobulin Constant Regions - genetics</subject><subject>Immunoglobulin gamma-Chains - genetics</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>Mice - embryology</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtLA0EUhQdRYnxU1sJUdhvnmZ0tLER8QcBCrSyWedxNVuYRZ3cD-fcmJIhWVhfuuefwnYvQBSUTSip-HXW-VhM-EZIfoDHlU1aIasoO0ZhwIgtKhDpGJ133SQgVVIoRGk2ZlELwMfp47fNg-yEDTg1uQxhimvtkBt9GPNchaMwMXoBerbFd6O0SImDrUwSHm5wCDmnoAEMweZ12qm9N1nl9ho4a7Ts4389T9P5w_3b3VMxeHp_vbmfFkjHeFyUxVjJTSQ6uVJYxqZzjQJtKOg2WWQu8ocpRDQ2U1oCjgighpFZSUdXwU3Szy10OJoCzEPusfb3MbdhQ1Em39V8ltot6nlY1ZyVTfOO_2vtz-hqg6-vQdha81xE23epSciErqv49ZISWlai2iZe_iX5Q9m_n3xfQhs8</recordid><startdate>19800211</startdate><enddate>19800211</enddate><creator>Zakut, R</creator><creator>Givol, D</creator><creator>Mory, Y Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800211</creationdate><title>Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library</title><author>Zakut, R ; Givol, D ; Mory, Y Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p223t-70bc52b953ed78c2258dd3e1f95daec2cce3f18d1aefe7cbed1408445a85818f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Neoplasm - genetics</topic><topic>Immunoglobulin Constant Regions - genetics</topic><topic>Immunoglobulin gamma-Chains - genetics</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>Mice - embryology</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zakut, R</creatorcontrib><creatorcontrib>Givol, D</creatorcontrib><creatorcontrib>Mory, Y Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zakut, R</au><au>Givol, D</au><au>Mory, Y Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1980-02-11</date><risdate>1980</risdate><volume>8</volume><issue>3</issue><spage>453</spage><epage>466</epage><pages>453-466</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>A mouse DNA clone containing the constant part of the immunoglobulin gamma 2b heavy chain was isolated from a mouse gene library. The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p gamma (11)7) containing a cDNA insert of the heavy chain constant region of the plasmacytoma MPC-11 (1). The Charon 4A clone contains a 14 kb insert which is cleaved by EcoRI into a 6.8 kb and 7.2 kb fragments, of which only the 6.8 kb contains the sequence for gamma 2b heavy chain. Restriction analysis and partial sequence of the insert in p gamma (11) 7 enabled us to obtain three fragments corresponding to the 5' (amino acid 161-302) middle (amino acid 302-443) and 3' (mostly non coding 107 bp) regions of the constant region. Restriction analysis of the Charon 4A clone and hybridisation to these nick translated fragments revealed that the gamma 2b constant region gene contains about 1.5 kb and has three intervening sequences.</abstract><cop>England</cop><pmid>6255443</pmid><doi>10.1093/nar/8.3.453</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1980-02, Vol.8 (3), p.453-466 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Neoplasm - genetics Immunoglobulin Constant Regions - genetics Immunoglobulin gamma-Chains - genetics Immunoglobulin Heavy Chains - genetics Mice - embryology Nucleic Acid Hybridization Plasmids |
| title | Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library |
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