Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization
B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to...
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| Veröffentlicht in: | Blood 2012-01, Vol.119 (4), p.978-989 |
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| creator | Park, Chung Hwang, Il-Young Sinha, Rajesh K. Kamenyeva, Olena Davis, Michael D. Kehrl, John H. |
| description | B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs. |
| doi_str_mv | 10.1182/blood-2011-06-364273 |
| format | Article |
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Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2011-06-364273</identifier><identifier>PMID: 22039261</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Animals ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; B-Lymphocytes - metabolism ; Biological and medical sciences ; Cell Adhesion ; Cells, Cultured ; Chemokines - metabolism ; Chemotaxis, Leukocyte ; Crosses, Genetic ; Down-Regulation ; Groin ; Hematologic and hematopoietic diseases ; Immunobiology ; Kinetics ; Lymph Nodes - anatomy & histology ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Lymph Nodes - metabolism ; Lymphatic System - anatomy & histology ; Lymphatic System - cytology ; Lymphatic System - immunology ; Lymphatic System - metabolism ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Biological ; Receptors, CCR7 - metabolism ; Receptors, Chemokine - metabolism ; Receptors, Lysosphingolipid - metabolism ; Signal Transduction ; Transendothelial and Transepithelial Migration</subject><ispartof>Blood, 2012-01, Vol.119 (4), p.978-989</ispartof><rights>2012 American Society of Hematology</rights><rights>2015 INIST-CNRS</rights><rights>2012 by The American Society of Hematology 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-a227d83fc92d43ab7a7afdb7269baa97c7d63a3ee5660e63b1d912c5a611861b3</citedby><cites>FETCH-LOGICAL-c492t-a227d83fc92d43ab7a7afdb7269baa97c7d63a3ee5660e63b1d912c5a611861b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25483098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22039261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Chung</creatorcontrib><creatorcontrib>Hwang, Il-Young</creatorcontrib><creatorcontrib>Sinha, Rajesh K.</creatorcontrib><creatorcontrib>Kamenyeva, Olena</creatorcontrib><creatorcontrib>Davis, Michael D.</creatorcontrib><creatorcontrib>Kehrl, John H.</creatorcontrib><title>Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization</title><title>Blood</title><addtitle>Blood</addtitle><description>B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. 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These results are summarized in a model of homeostatic trafficking of B cells through LNs.</description><subject>Animals</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cells, Cultured</subject><subject>Chemokines - metabolism</subject><subject>Chemotaxis, Leukocyte</subject><subject>Crosses, Genetic</subject><subject>Down-Regulation</subject><subject>Groin</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Immunobiology</subject><subject>Kinetics</subject><subject>Lymph Nodes - anatomy & histology</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - metabolism</subject><subject>Lymphatic System - anatomy & histology</subject><subject>Lymphatic System - cytology</subject><subject>Lymphatic System - immunology</subject><subject>Lymphatic System - metabolism</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Models, Biological</subject><subject>Receptors, CCR7 - metabolism</subject><subject>Receptors, Chemokine - metabolism</subject><subject>Receptors, Lysosphingolipid - metabolism</subject><subject>Signal Transduction</subject><subject>Transendothelial and Transepithelial Migration</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuO1DAQjBCIHRb-ACFfEKeAH4mdXJDYFS9pJC5wtjp2Z2JI7MH2LApfj4cZduHCqeXuqupyV1U9ZfQlYx1_Ncwh2JpTxmoqayEbrsS9asNa3tWUcnq_2lBaJk2v2EX1KKWvlLJG8PZhdcE5FT2XbFP92K7LfiI-WCRXZD4-glkzkhxhHJ355vyOuERM8Km0nEdLhpWAhxyWY7VkcrtpXonFPXqLPpPDPnhiJlwC5MIxGUrTYkKfXHY_IbvgH1cPRpgTPjnXy-rLu7efrz_U20_vP16_2dam6XmugXNlOzGanttGwKBAwWgHxWU_APTKKCsFCMRWSopSDMz2jJsWZLmRZIO4rF6fdPeHYUFrir8Is95Ht0BcdQCn_514N-lduNGCK6Y4KwIvzgIxfD9gynpxyeA8g8dwSLpnXataKnlBNiekiSGliOPtFkb1MTL9OzJ9jExTqU-RFdqzvx3ekv5kVADPzwBIBuYxgjcu3eHaphO07-6-iuWeNw6jTsahN2hdRJO1De7_Tn4B38m5ew</recordid><startdate>20120126</startdate><enddate>20120126</enddate><creator>Park, Chung</creator><creator>Hwang, Il-Young</creator><creator>Sinha, Rajesh K.</creator><creator>Kamenyeva, Olena</creator><creator>Davis, Michael D.</creator><creator>Kehrl, John H.</creator><general>Elsevier Inc</general><general>Americain Society of Hematology</general><general>American Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120126</creationdate><title>Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization</title><author>Park, Chung ; Hwang, Il-Young ; Sinha, Rajesh K. ; Kamenyeva, Olena ; Davis, Michael D. ; Kehrl, John H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-a227d83fc92d43ab7a7afdb7269baa97c7d63a3ee5660e63b1d912c5a611861b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cells, Cultured</topic><topic>Chemokines - metabolism</topic><topic>Chemotaxis, Leukocyte</topic><topic>Crosses, Genetic</topic><topic>Down-Regulation</topic><topic>Groin</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Immunobiology</topic><topic>Kinetics</topic><topic>Lymph Nodes - anatomy & histology</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - metabolism</topic><topic>Lymphatic System - anatomy & histology</topic><topic>Lymphatic System - cytology</topic><topic>Lymphatic System - immunology</topic><topic>Lymphatic System - metabolism</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Models, Biological</topic><topic>Receptors, CCR7 - metabolism</topic><topic>Receptors, Chemokine - metabolism</topic><topic>Receptors, Lysosphingolipid - metabolism</topic><topic>Signal Transduction</topic><topic>Transendothelial and Transepithelial Migration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Chung</creatorcontrib><creatorcontrib>Hwang, Il-Young</creatorcontrib><creatorcontrib>Sinha, Rajesh K.</creatorcontrib><creatorcontrib>Kamenyeva, Olena</creatorcontrib><creatorcontrib>Davis, Michael D.</creatorcontrib><creatorcontrib>Kehrl, John H.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Chung</au><au>Hwang, Il-Young</au><au>Sinha, Rajesh K.</au><au>Kamenyeva, Olena</au><au>Davis, Michael D.</au><au>Kehrl, John H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>2012-01-26</date><risdate>2012</risdate><volume>119</volume><issue>4</issue><spage>978</spage><epage>989</epage><pages>978-989</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>22039261</pmid><doi>10.1182/blood-2011-06-364273</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 2012-01, Vol.119 (4), p.978-989 |
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| subjects | Animals B-Lymphocytes - cytology B-Lymphocytes - immunology B-Lymphocytes - metabolism Biological and medical sciences Cell Adhesion Cells, Cultured Chemokines - metabolism Chemotaxis, Leukocyte Crosses, Genetic Down-Regulation Groin Hematologic and hematopoietic diseases Immunobiology Kinetics Lymph Nodes - anatomy & histology Lymph Nodes - cytology Lymph Nodes - immunology Lymph Nodes - metabolism Lymphatic System - anatomy & histology Lymphatic System - cytology Lymphatic System - immunology Lymphatic System - metabolism Medical sciences Mice Mice, Inbred C57BL Mice, Transgenic Models, Biological Receptors, CCR7 - metabolism Receptors, Chemokine - metabolism Receptors, Lysosphingolipid - metabolism Signal Transduction Transendothelial and Transepithelial Migration |
| title | Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization |
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