Wnt-RhoA signaling is involved in dental enamel development
Peng L, Li Y, Shusterman K, Kuehl M, Gibson CW. Wnt‐RhoA signaling is involved in dental enamel development. Eur J Oral Sci 2011; 119 (Suppl. 1): 41–49. © 2011 Eur J Oral Sci Transgenic mice that express dominant‐negative RhoA (RhoADN) in ameloblasts have hypoplastic enamel with defects in molar c...
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| description | Peng L, Li Y, Shusterman K, Kuehl M, Gibson CW. Wnt‐RhoA signaling is involved in dental enamel development.
Eur J Oral Sci 2011; 119 (Suppl. 1): 41–49. © 2011 Eur J Oral Sci
Transgenic mice that express dominant‐negative RhoA (RhoADN) in ameloblasts have hypoplastic enamel with defects in molar cusps. β‐catenin and Wnt5a were up‐regulated in enamel organs of RhoADN transgenic mice, which indicated that both canonical and non‐canonical Wnt pathways are implicated in the process of enamel defect formation. It was hypothesized that expression of RhoADN in ameloblasts interfered with normal enamel development through the pathways that were induced by fluoride. The Wnt and RhoA pathways were further investigated in an ameloblast‐lineage cell line (ALC) by treatment with sodium fluoride (NaF). The activities of RhoA and Rho‐associated protein kinase (ROCK) II decreased significantly by 8–12 hours, similar to decreased activity in RhoADN transgenic mice. Both canonical and non‐canonical Wnt pathways were activated by treatment with NaF, which was verified by western blotting and the β‐catenin‐TCF/LEF (T cell factor lymphanoid/enhancer factor) reporter gene (TOPflash) assay. β‐catenin localization to both cytoplasm and nucleus was up‐regulated in NaF‐treated ALC, while Gsk‐3β, the negative regulator of the Wnt pathway, showed a decreased pattern of expression. The current results indicate that both Wnt and RhoA pathways are implicated in fluoride‐induced signaling transductions in the ALC as well as in the development of enamel defects in RhoADN transgenic mice. |
| doi_str_mv | 10.1111/j.1600-0722.2011.00880.x |
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Eur J Oral Sci 2011; 119 (Suppl. 1): 41–49. © 2011 Eur J Oral Sci
Transgenic mice that express dominant‐negative RhoA (RhoADN) in ameloblasts have hypoplastic enamel with defects in molar cusps. β‐catenin and Wnt5a were up‐regulated in enamel organs of RhoADN transgenic mice, which indicated that both canonical and non‐canonical Wnt pathways are implicated in the process of enamel defect formation. It was hypothesized that expression of RhoADN in ameloblasts interfered with normal enamel development through the pathways that were induced by fluoride. The Wnt and RhoA pathways were further investigated in an ameloblast‐lineage cell line (ALC) by treatment with sodium fluoride (NaF). The activities of RhoA and Rho‐associated protein kinase (ROCK) II decreased significantly by 8–12 hours, similar to decreased activity in RhoADN transgenic mice. Both canonical and non‐canonical Wnt pathways were activated by treatment with NaF, which was verified by western blotting and the β‐catenin‐TCF/LEF (T cell factor lymphanoid/enhancer factor) reporter gene (TOPflash) assay. β‐catenin localization to both cytoplasm and nucleus was up‐regulated in NaF‐treated ALC, while Gsk‐3β, the negative regulator of the Wnt pathway, showed a decreased pattern of expression. The current results indicate that both Wnt and RhoA pathways are implicated in fluoride‐induced signaling transductions in the ALC as well as in the development of enamel defects in RhoADN transgenic mice.</description><identifier>ISSN: 0909-8836</identifier><identifier>EISSN: 1600-0722</identifier><identifier>DOI: 10.1111/j.1600-0722.2011.00880.x</identifier><identifier>PMID: 22243225</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Ameloblasts - drug effects ; Ameloblasts - physiology ; Amelogenesis - genetics ; Amelogenin - biosynthesis ; Amelogenin - genetics ; Animals ; beta Catenin - biosynthesis ; beta Catenin - genetics ; Cell Line ; dental enamel ; Dental Enamel Hypoplasia - genetics ; Dentistry ; Enamel Organ - drug effects ; Enamel Organ - physiology ; Gene Expression Regulation, Developmental ; Genes, Reporter ; GTP-Binding Protein Regulators - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; rho GTP-Binding Proteins - genetics ; rho GTP-Binding Proteins - physiology ; rho-Associated Kinases - genetics ; RhoA ; rhoA GTP-Binding Protein - genetics ; rhoA GTP-Binding Protein - physiology ; RhoADN transgenic mice ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Sodium Fluoride - pharmacology ; Wnt ; Wnt Proteins - biosynthesis ; Wnt Proteins - genetics ; Wnt Signaling Pathway - drug effects ; Wnt Signaling Pathway - genetics ; Wnt-5a Protein</subject><ispartof>European journal of oral sciences, 2011-12, Vol.119 (s1), p.41-49</ispartof><rights>2011 Eur J Oral Sci</rights><rights>2011 Eur J Oral Sci.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6090-590b0854bc862d0611c561d37604d80d7498f9f34d719a114692ee19b0678b8a3</citedby><cites>FETCH-LOGICAL-c6090-590b0854bc862d0611c561d37604d80d7498f9f34d719a114692ee19b0678b8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0722.2011.00880.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0722.2011.00880.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22243225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Li</creatorcontrib><creatorcontrib>Li, Yong</creatorcontrib><creatorcontrib>Shusterman, Kate</creatorcontrib><creatorcontrib>Kuehl, Melissa</creatorcontrib><creatorcontrib>Gibson, Carolyn W.</creatorcontrib><title>Wnt-RhoA signaling is involved in dental enamel development</title><title>European journal of oral sciences</title><addtitle>Eur J Oral Sci</addtitle><description>Peng L, Li Y, Shusterman K, Kuehl M, Gibson CW. Wnt‐RhoA signaling is involved in dental enamel development.
Eur J Oral Sci 2011; 119 (Suppl. 1): 41–49. © 2011 Eur J Oral Sci
Transgenic mice that express dominant‐negative RhoA (RhoADN) in ameloblasts have hypoplastic enamel with defects in molar cusps. β‐catenin and Wnt5a were up‐regulated in enamel organs of RhoADN transgenic mice, which indicated that both canonical and non‐canonical Wnt pathways are implicated in the process of enamel defect formation. It was hypothesized that expression of RhoADN in ameloblasts interfered with normal enamel development through the pathways that were induced by fluoride. The Wnt and RhoA pathways were further investigated in an ameloblast‐lineage cell line (ALC) by treatment with sodium fluoride (NaF). The activities of RhoA and Rho‐associated protein kinase (ROCK) II decreased significantly by 8–12 hours, similar to decreased activity in RhoADN transgenic mice. Both canonical and non‐canonical Wnt pathways were activated by treatment with NaF, which was verified by western blotting and the β‐catenin‐TCF/LEF (T cell factor lymphanoid/enhancer factor) reporter gene (TOPflash) assay. β‐catenin localization to both cytoplasm and nucleus was up‐regulated in NaF‐treated ALC, while Gsk‐3β, the negative regulator of the Wnt pathway, showed a decreased pattern of expression. The current results indicate that both Wnt and RhoA pathways are implicated in fluoride‐induced signaling transductions in the ALC as well as in the development of enamel defects in RhoADN transgenic mice.</description><subject>Ameloblasts - drug effects</subject><subject>Ameloblasts - physiology</subject><subject>Amelogenesis - genetics</subject><subject>Amelogenin - biosynthesis</subject><subject>Amelogenin - genetics</subject><subject>Animals</subject><subject>beta Catenin - biosynthesis</subject><subject>beta Catenin - genetics</subject><subject>Cell Line</subject><subject>dental enamel</subject><subject>Dental Enamel Hypoplasia - genetics</subject><subject>Dentistry</subject><subject>Enamel Organ - drug effects</subject><subject>Enamel Organ - physiology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Reporter</subject><subject>GTP-Binding Protein Regulators - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>rho GTP-Binding Proteins - genetics</subject><subject>rho GTP-Binding Proteins - physiology</subject><subject>rho-Associated Kinases - genetics</subject><subject>RhoA</subject><subject>rhoA GTP-Binding Protein - genetics</subject><subject>rhoA GTP-Binding Protein - physiology</subject><subject>RhoADN transgenic mice</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Sodium Fluoride - pharmacology</subject><subject>Wnt</subject><subject>Wnt Proteins - biosynthesis</subject><subject>Wnt Proteins - genetics</subject><subject>Wnt Signaling Pathway - drug effects</subject><subject>Wnt Signaling Pathway - genetics</subject><subject>Wnt-5a Protein</subject><issn>0909-8836</issn><issn>1600-0722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUV1v0zAUtRCIlcFfQHmDl4R77cQfAiFN01YQg0kMNN6unMTtXJykxG3p_j3uOip4Qfjl2veec3zsw1iGUGBarxYFSoAcFOcFB8QCQGsotg_Y5DB4yCZgwORaC3nEnsS4AECBRj1mR5zzUnBeTdjr636Vf74ZTrLo570Nvp9nPma-3wxh49q0yVrXr2zIXG87F9Jp48Kw7FLzKXs0syG6Z_f1mH09P_ty-i6_uJy-Pz25yBuZHOSVgRp0VdaNlrwFidhUEluhJJSthlaVRs_MTJStQmMRS2m4c2hqkErX2opj9navu1zXnWubdPVoAy1H39nxlgbr6e9J729oPmxIcJX-RSeBF_cC4_Bj7eKKOh8bF4Lt3bCOZFAlfyBMQr78JxKFMQYEakhQvYc24xDj6GYHQwi0S4kWtAuDdmHQLiW6S4m2ifr8zwcdiL9jSYA3e8BPH9ztfwvT2eWVvrOW7-k-rtz2QLfjd5JKqIquP03pI5x_41cfpqTEL7cardo</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Peng, Li</creator><creator>Li, Yong</creator><creator>Shusterman, Kate</creator><creator>Kuehl, Melissa</creator><creator>Gibson, Carolyn W.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201112</creationdate><title>Wnt-RhoA signaling is involved in dental enamel development</title><author>Peng, Li ; Li, Yong ; Shusterman, Kate ; Kuehl, Melissa ; Gibson, Carolyn W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6090-590b0854bc862d0611c561d37604d80d7498f9f34d719a114692ee19b0678b8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Ameloblasts - drug effects</topic><topic>Ameloblasts - physiology</topic><topic>Amelogenesis - genetics</topic><topic>Amelogenin - biosynthesis</topic><topic>Amelogenin - genetics</topic><topic>Animals</topic><topic>beta Catenin - biosynthesis</topic><topic>beta Catenin - genetics</topic><topic>Cell Line</topic><topic>dental enamel</topic><topic>Dental Enamel Hypoplasia - genetics</topic><topic>Dentistry</topic><topic>Enamel Organ - drug effects</topic><topic>Enamel Organ - physiology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes, Reporter</topic><topic>GTP-Binding Protein Regulators - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>rho GTP-Binding Proteins - genetics</topic><topic>rho GTP-Binding Proteins - physiology</topic><topic>rho-Associated Kinases - genetics</topic><topic>RhoA</topic><topic>rhoA GTP-Binding Protein - genetics</topic><topic>rhoA GTP-Binding Protein - physiology</topic><topic>RhoADN transgenic mice</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Sodium Fluoride - pharmacology</topic><topic>Wnt</topic><topic>Wnt Proteins - biosynthesis</topic><topic>Wnt Proteins - genetics</topic><topic>Wnt Signaling Pathway - drug effects</topic><topic>Wnt Signaling Pathway - genetics</topic><topic>Wnt-5a Protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Li</creatorcontrib><creatorcontrib>Li, Yong</creatorcontrib><creatorcontrib>Shusterman, Kate</creatorcontrib><creatorcontrib>Kuehl, Melissa</creatorcontrib><creatorcontrib>Gibson, Carolyn W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European journal of oral sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Li</au><au>Li, Yong</au><au>Shusterman, Kate</au><au>Kuehl, Melissa</au><au>Gibson, Carolyn W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wnt-RhoA signaling is involved in dental enamel development</atitle><jtitle>European journal of oral sciences</jtitle><addtitle>Eur J Oral Sci</addtitle><date>2011-12</date><risdate>2011</risdate><volume>119</volume><issue>s1</issue><spage>41</spage><epage>49</epage><pages>41-49</pages><issn>0909-8836</issn><eissn>1600-0722</eissn><abstract>Peng L, Li Y, Shusterman K, Kuehl M, Gibson CW. Wnt‐RhoA signaling is involved in dental enamel development.
Eur J Oral Sci 2011; 119 (Suppl. 1): 41–49. © 2011 Eur J Oral Sci
Transgenic mice that express dominant‐negative RhoA (RhoADN) in ameloblasts have hypoplastic enamel with defects in molar cusps. β‐catenin and Wnt5a were up‐regulated in enamel organs of RhoADN transgenic mice, which indicated that both canonical and non‐canonical Wnt pathways are implicated in the process of enamel defect formation. It was hypothesized that expression of RhoADN in ameloblasts interfered with normal enamel development through the pathways that were induced by fluoride. The Wnt and RhoA pathways were further investigated in an ameloblast‐lineage cell line (ALC) by treatment with sodium fluoride (NaF). The activities of RhoA and Rho‐associated protein kinase (ROCK) II decreased significantly by 8–12 hours, similar to decreased activity in RhoADN transgenic mice. Both canonical and non‐canonical Wnt pathways were activated by treatment with NaF, which was verified by western blotting and the β‐catenin‐TCF/LEF (T cell factor lymphanoid/enhancer factor) reporter gene (TOPflash) assay. β‐catenin localization to both cytoplasm and nucleus was up‐regulated in NaF‐treated ALC, while Gsk‐3β, the negative regulator of the Wnt pathway, showed a decreased pattern of expression. The current results indicate that both Wnt and RhoA pathways are implicated in fluoride‐induced signaling transductions in the ALC as well as in the development of enamel defects in RhoADN transgenic mice.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22243225</pmid><doi>10.1111/j.1600-0722.2011.00880.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Ameloblasts - drug effects Ameloblasts - physiology Amelogenesis - genetics Amelogenin - biosynthesis Amelogenin - genetics Animals beta Catenin - biosynthesis beta Catenin - genetics Cell Line dental enamel Dental Enamel Hypoplasia - genetics Dentistry Enamel Organ - drug effects Enamel Organ - physiology Gene Expression Regulation, Developmental Genes, Reporter GTP-Binding Protein Regulators - metabolism Mice Mice, Inbred C57BL Mice, Knockout rho GTP-Binding Proteins - genetics rho GTP-Binding Proteins - physiology rho-Associated Kinases - genetics RhoA rhoA GTP-Binding Protein - genetics rhoA GTP-Binding Protein - physiology RhoADN transgenic mice Signal Transduction - drug effects Signal Transduction - genetics Sodium Fluoride - pharmacology Wnt Wnt Proteins - biosynthesis Wnt Proteins - genetics Wnt Signaling Pathway - drug effects Wnt Signaling Pathway - genetics Wnt-5a Protein |
| title | Wnt-RhoA signaling is involved in dental enamel development |
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