An orthogonal purification strategy for isolating crosslinked domains of modular synthases

Chemo-enzymatic methods for covalently crosslinking carrier proteins with partner enzymes within modular synthases hold promise for elucidating and engineering metabolic pathways. Our efforts to crystallize the ACP–KS complexes of fatty acid synthases have been complicated by difficulties in the pur...

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Veröffentlicht in:	Bioorganic & medicinal chemistry 2008-05, Vol.18 (10), p.3039-3042
Hauptverfasser:	Haushalter, Robert W., Worthington, Andrew S., Hur, Gene H., Burkart, Michael D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylates - chemistry Acyl Carrier Protein - chemistry Acyl Carrier Protein - isolation & purification Analytical, structural and metabolic biochemistry Biological and medical sciences Chemo-enzymatic synthesis Coenzyme A - chemistry Coenzyme A - genetics Coenzyme A - isolation & purification Cross-Linking Reagents - chemistry Enzymes and enzyme inhibitors Escherichia - enzymology Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - isolation & purification Fatty Acid Synthases - chemistry Fatty Acid Synthases - genetics Fatty Acid Synthases - isolation & purification Fundamental and applied biological sciences. Psychology Models, Molecular Pantetheine - analogs & derivatives Pantetheine - chemistry Polyketide Synthases - chemistry Polyketide Synthases - genetics Polyketide Synthases - isolation & purification Polymerase Chain Reaction Protein Conformation Protein modification Protein purification Protein Structure, Tertiary Transferases
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