Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can b...

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Veröffentlicht in:Biomedical optics express 2012-02, Vol.3 (2), p.354-359
Hauptverfasser: Watanabe, Koyo, Matsuura, Koji, Kawata, Fukukazu, Nagata, Kotaro, Ning, Jun, Kano, Hiroshi
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Cell Studies
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container_issue 2
container_start_page 354
container_title Biomedical optics express
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creator Watanabe, Koyo
Matsuura, Koji
Kawata, Fukukazu
Nagata, Kotaro
Ning, Jun
Kano, Hiroshi
description We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.
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title Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
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