In vitro antioxidant, anticoagulant and antimicrobial activity and in inhibition of cancer cell proliferation by xylan extracted from corn cobs
Xylan is one of most abundant polymer after cellulose. However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhan...
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| Veröffentlicht in: | International journal of molecular sciences 2012-01, Vol.13 (1), p.409-426 |
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| creator | Melo-Silveira, Raniere Fagundes Fidelis, Gabriel Pereira Costa, Mariana Santana Santos Pereira Telles, Cinthia Beatrice Silva Dantas-Santos, Nednaldo de Oliveira Elias, Susana Ribeiro, Vanessa Bley Barth, Afonso Luis Macedo, Alexandre José Leite, Edda Lisboa Rocha, Hugo Alexandre Oliveira |
| description | Xylan is one of most abundant polymer after cellulose. However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ultrasonication. Physical chemistry and infrared analyses showed 130 kDa heteroxylan containing mainly xylose:arabinose: galactose:glucose (5.0:1.5:2.0:1.2). Xylan obtained exhibited total antioxidant activity corresponding to 48.5 mg of ascorbic acid equivalent/g of xylan. Furthermore, xylan displayed high ferric chelating activity (70%) at 2 mg/mL. Xylan also showed anticoagulant activity in aPTT test. In antimicrobial assay, the polysaccharide significantly inhibited bacterial growth of Klebsiella pneumoniae. In a test with normal and tumor human cells, after 72 h, only HeLa tumor cell proliferation was inhibited (p < 0.05) in a dose-dependent manner by xylan, reaching saturation at around 2 mg/mL, whereas 3T3 normal cell proliferation was not affected. The results suggest that it has potential clinical applications as antioxidant, anticoagulant, antimicrobial and antiproliferative compounds. |
| doi_str_mv | 10.3390/ijms13010409 |
| format | Article |
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However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ultrasonication. Physical chemistry and infrared analyses showed 130 kDa heteroxylan containing mainly xylose:arabinose: galactose:glucose (5.0:1.5:2.0:1.2). Xylan obtained exhibited total antioxidant activity corresponding to 48.5 mg of ascorbic acid equivalent/g of xylan. Furthermore, xylan displayed high ferric chelating activity (70%) at 2 mg/mL. Xylan also showed anticoagulant activity in aPTT test. In antimicrobial assay, the polysaccharide significantly inhibited bacterial growth of Klebsiella pneumoniae. In a test with normal and tumor human cells, after 72 h, only HeLa tumor cell proliferation was inhibited (p < 0.05) in a dose-dependent manner by xylan, reaching saturation at around 2 mg/mL, whereas 3T3 normal cell proliferation was not affected. The results suggest that it has potential clinical applications as antioxidant, anticoagulant, antimicrobial and antiproliferative compounds.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms13010409</identifier><identifier>PMID: 22312261</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>3T3 Cells ; Animals ; Anti-Infective Agents - chemistry ; Anti-Infective Agents - pharmacology ; Anticoagulants ; Anticoagulants - chemistry ; Anticoagulants - metabolism ; Antimicrobial activity ; Antimicrobial agents ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - metabolism ; Arabinose ; Ascorbic acid ; Biochemistry ; Biofilms - drug effects ; Biopolymers ; Cancer ; Carbohydrates ; Cardiovascular disease ; Cell growth ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - drug effects ; Cellulose ; Electronic mail systems ; Food ; Galactose ; Glucose ; HeLa Cells ; Humans ; Klebsiella pneumoniae ; Klebsiella pneumoniae - drug effects ; Klebsiella pneumoniae - physiology ; Mice ; Partial Thromboplastin Time ; Pharmacy ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Polysaccharides ; Staphylococcus epidermidis - drug effects ; Staphylococcus epidermidis - physiology ; Superoxides - chemistry ; Therapeutic applications ; Thromboplastin - chemistry ; Thromboplastin - metabolism ; Toxicity ; Tumor cells ; Tumors ; Xylan ; Xylans - chemistry ; Xylans - pharmacology ; Xylose ; Zea mays - chemistry ; Zea mays - metabolism</subject><ispartof>International journal of molecular sciences, 2012-01, Vol.13 (1), p.409-426</ispartof><rights>Copyright MDPI AG 2012</rights><rights>2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-abb8593393e74d8606a52502eaf53052f76a004f2c72cf1f2a3ec96299a12673</citedby><cites>FETCH-LOGICAL-c510t-abb8593393e74d8606a52502eaf53052f76a004f2c72cf1f2a3ec96299a12673</cites><orcidid>0000-0003-2252-1221</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269695/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269695/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22312261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Melo-Silveira, Raniere Fagundes</creatorcontrib><creatorcontrib>Fidelis, Gabriel Pereira</creatorcontrib><creatorcontrib>Costa, Mariana Santana Santos Pereira</creatorcontrib><creatorcontrib>Telles, Cinthia Beatrice Silva</creatorcontrib><creatorcontrib>Dantas-Santos, Nednaldo</creatorcontrib><creatorcontrib>de Oliveira Elias, Susana</creatorcontrib><creatorcontrib>Ribeiro, Vanessa Bley</creatorcontrib><creatorcontrib>Barth, Afonso Luis</creatorcontrib><creatorcontrib>Macedo, Alexandre José</creatorcontrib><creatorcontrib>Leite, Edda Lisboa</creatorcontrib><creatorcontrib>Rocha, Hugo Alexandre Oliveira</creatorcontrib><title>In vitro antioxidant, anticoagulant and antimicrobial activity and in inhibition of cancer cell proliferation by xylan extracted from corn cobs</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Xylan is one of most abundant polymer after cellulose. However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ultrasonication. Physical chemistry and infrared analyses showed 130 kDa heteroxylan containing mainly xylose:arabinose: galactose:glucose (5.0:1.5:2.0:1.2). Xylan obtained exhibited total antioxidant activity corresponding to 48.5 mg of ascorbic acid equivalent/g of xylan. Furthermore, xylan displayed high ferric chelating activity (70%) at 2 mg/mL. Xylan also showed anticoagulant activity in aPTT test. In antimicrobial assay, the polysaccharide significantly inhibited bacterial growth of Klebsiella pneumoniae. In a test with normal and tumor human cells, after 72 h, only HeLa tumor cell proliferation was inhibited (p < 0.05) in a dose-dependent manner by xylan, reaching saturation at around 2 mg/mL, whereas 3T3 normal cell proliferation was not affected. The results suggest that it has potential clinical applications as antioxidant, anticoagulant, antimicrobial and antiproliferative compounds.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Anti-Infective Agents - chemistry</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Anticoagulants</subject><subject>Anticoagulants - chemistry</subject><subject>Anticoagulants - metabolism</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - metabolism</subject><subject>Arabinose</subject><subject>Ascorbic acid</subject><subject>Biochemistry</subject><subject>Biofilms - drug effects</subject><subject>Biopolymers</subject><subject>Cancer</subject><subject>Carbohydrates</subject><subject>Cardiovascular disease</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cellulose</subject><subject>Electronic mail systems</subject><subject>Food</subject><subject>Galactose</subject><subject>Glucose</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Klebsiella pneumoniae</subject><subject>Klebsiella pneumoniae - drug effects</subject><subject>Klebsiella pneumoniae - physiology</subject><subject>Mice</subject><subject>Partial Thromboplastin Time</subject><subject>Pharmacy</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Polysaccharides</subject><subject>Staphylococcus epidermidis - drug effects</subject><subject>Staphylococcus epidermidis - physiology</subject><subject>Superoxides - chemistry</subject><subject>Therapeutic applications</subject><subject>Thromboplastin - chemistry</subject><subject>Thromboplastin - metabolism</subject><subject>Toxicity</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Xylan</subject><subject>Xylans - chemistry</subject><subject>Xylans - pharmacology</subject><subject>Xylose</subject><subject>Zea mays - chemistry</subject><subject>Zea mays - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdUU1vGyEQRVWq5qO95Rwh5dJD3A6DYc0lUhS1aaRIveSOWBYSrF1wYDeyf0X-crGTRm4lxMzwHo95DCGnDL5xruB7WA6FcWAwB_WBHLE54gxANgd7-SE5LmUJgByF-kQOETlDlOyIvNxG-hzGnKiJY0jr0NV4sStsMg9TX7NadbuTIdic2mB6auwY6rXNDgqxrsfQhioQafLUmmhdptb1PV3l1AfvstmB7YauN1WTuvWYq4jrqM9poDblWLe2fCYfvemL-_IWT8j9zx_3179md79vbq-v7mZWMBhnpm0XQlX_3DXzbiFBGoEC0BkvOAj0jTQAc4-2QeuZR8OdVRKVMgxlw0_I5avsamoH11kXazu9XuUwmLzRyQT9LxLDo35Iz5qjVFKJKvD1TSCnp8mVUQ-hbA2b6NJUNAOEBZcMtm-d_0ddpinH6k4zgRIbJiWrrItXVv3iUrLz780w0NtB6_1BV_rZvoF38t_J8j8A86b8</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Melo-Silveira, Raniere Fagundes</creator><creator>Fidelis, Gabriel Pereira</creator><creator>Costa, Mariana Santana Santos Pereira</creator><creator>Telles, Cinthia Beatrice Silva</creator><creator>Dantas-Santos, Nednaldo</creator><creator>de Oliveira Elias, Susana</creator><creator>Ribeiro, Vanessa Bley</creator><creator>Barth, Afonso Luis</creator><creator>Macedo, Alexandre José</creator><creator>Leite, Edda Lisboa</creator><creator>Rocha, Hugo Alexandre Oliveira</creator><general>MDPI AG</general><general>Molecular Diversity Preservation International (MDPI)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2252-1221</orcidid></search><sort><creationdate>20120101</creationdate><title>In vitro antioxidant, anticoagulant and antimicrobial activity and in inhibition of cancer cell proliferation by xylan extracted from corn cobs</title><author>Melo-Silveira, Raniere Fagundes ; Fidelis, Gabriel Pereira ; Costa, Mariana Santana Santos Pereira ; Telles, Cinthia Beatrice Silva ; Dantas-Santos, Nednaldo ; de Oliveira Elias, Susana ; Ribeiro, Vanessa Bley ; Barth, Afonso Luis ; Macedo, Alexandre José ; Leite, Edda Lisboa ; Rocha, Hugo Alexandre Oliveira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-abb8593393e74d8606a52502eaf53052f76a004f2c72cf1f2a3ec96299a12673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Anti-Infective Agents - 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However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ultrasonication. Physical chemistry and infrared analyses showed 130 kDa heteroxylan containing mainly xylose:arabinose: galactose:glucose (5.0:1.5:2.0:1.2). Xylan obtained exhibited total antioxidant activity corresponding to 48.5 mg of ascorbic acid equivalent/g of xylan. Furthermore, xylan displayed high ferric chelating activity (70%) at 2 mg/mL. Xylan also showed anticoagulant activity in aPTT test. In antimicrobial assay, the polysaccharide significantly inhibited bacterial growth of Klebsiella pneumoniae. In a test with normal and tumor human cells, after 72 h, only HeLa tumor cell proliferation was inhibited (p < 0.05) in a dose-dependent manner by xylan, reaching saturation at around 2 mg/mL, whereas 3T3 normal cell proliferation was not affected. The results suggest that it has potential clinical applications as antioxidant, anticoagulant, antimicrobial and antiproliferative compounds.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>22312261</pmid><doi>10.3390/ijms13010409</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0003-2252-1221</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | 3T3 Cells Animals Anti-Infective Agents - chemistry Anti-Infective Agents - pharmacology Anticoagulants Anticoagulants - chemistry Anticoagulants - metabolism Antimicrobial activity Antimicrobial agents Antioxidants Antioxidants - chemistry Antioxidants - metabolism Arabinose Ascorbic acid Biochemistry Biofilms - drug effects Biopolymers Cancer Carbohydrates Cardiovascular disease Cell growth Cell Line, Tumor Cell proliferation Cell Proliferation - drug effects Cellulose Electronic mail systems Food Galactose Glucose HeLa Cells Humans Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - physiology Mice Partial Thromboplastin Time Pharmacy Plant Extracts - chemistry Plant Extracts - pharmacology Polysaccharides Staphylococcus epidermidis - drug effects Staphylococcus epidermidis - physiology Superoxides - chemistry Therapeutic applications Thromboplastin - chemistry Thromboplastin - metabolism Toxicity Tumor cells Tumors Xylan Xylans - chemistry Xylans - pharmacology Xylose Zea mays - chemistry Zea mays - metabolism |
| title | In vitro antioxidant, anticoagulant and antimicrobial activity and in inhibition of cancer cell proliferation by xylan extracted from corn cobs |
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