Encapsulation of Organic Molecules in Calcium Phosphate Nanocomposite Particles for Intracellular Imaging and Drug Delivery
Encapsulation of imaging agents and drugs in calcium phosphate nanoparticles (CPNPs) has potential as a nontoxic, bioresorbable vehicle for drug delivery to cells and tumors. The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and thera...
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| Veröffentlicht in: | Nano letters 2008-12, Vol.8 (12), p.4108-4115 |
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| creator | Morgan, Thomas T Muddana, Hari S Altinoǧlu, Erhan İ Rouse, Sarah M Tabaković, Amra Tabouillot, Tristan Russin, Timothy J Shanmugavelandy, Sriram S Butler, Peter J Eklund, Peter C Yun, Jong K Kester, Mark Adair, James H |
| description | Encapsulation of imaging agents and drugs in calcium phosphate nanoparticles (CPNPs) has potential as a nontoxic, bioresorbable vehicle for drug delivery to cells and tumors. The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention. |
| doi_str_mv | 10.1021/nl8019888 |
| format | Article |
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The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl8019888</identifier><identifier>PMID: 19367837</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Calcium Phosphates - chemistry ; Cattle ; Condensed matter: structure, mechanical and thermal properties ; Cross-disciplinary physics: materials science; rheology ; Diffusion in nanoscale solids ; Diffusion in solids ; Drug Carriers ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Materials science ; Methods. Procedures. 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The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Calcium Phosphates - chemistry</subject><subject>Cattle</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Diffusion in nanoscale solids</subject><subject>Diffusion in solids</subject><subject>Drug Carriers</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Materials science</subject><subject>Methods. Procedures. Technologies</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanocomposites</subject><subject>Nanocrystalline materials</subject><subject>Nanoscale materials and structures: fabrication and characterization</subject><subject>Organic Chemicals - chemistry</subject><subject>Others</subject><subject>Physics</subject><subject>Transport properties of condensed matter (nonelectronic)</subject><subject>Various methods and equipments</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1rFTEUhkOp2Fpd9A-UbIq4uJpM5iPZFOS2aqHaLnQdzmSSuSmZZJrMFIp_3gy9TBVcJeech_d8vAidUvKRkoJ-8o4TKjjnB-iYVoxsaiGKw_XPyyP0JqV7QohgFXmNjqhgdcNZc4x-X3kFY5odTDZ4HAy-jT14q_D34LSanU7YerwFp-w84LtdSOMOJo1_gA8qDGNINkd3ECerFtiEiK_9FEFp57Jsjgbore8x-A5fxrnHl9rZRx2f3qJXBlzS7_bvCfr15ern9tvm5vbr9fbzzQYqRqdNU7KKCaKKIi_btkUrOBFGKKoZyXndatERaJhhBspSCKNV0zUVlKymBLqOnaCLZ91xbgfdKb2M5-QY7QDxSQaw8t-KtzvZh0fJirqpWZEF3u8FYniYdZrkYNOyH3gd5iSbqqxKQcqF_PBMqhhSitqsXSiRi1dy9SqzZ3-P9ULuzcnA-R6ApMCZCF7ZtHIFEbzi2eOVA5XkfZijz9f8T8M_Vi2qdQ</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Morgan, Thomas T</creator><creator>Muddana, Hari S</creator><creator>Altinoǧlu, Erhan İ</creator><creator>Rouse, Sarah M</creator><creator>Tabaković, Amra</creator><creator>Tabouillot, Tristan</creator><creator>Russin, Timothy J</creator><creator>Shanmugavelandy, Sriram S</creator><creator>Butler, Peter J</creator><creator>Eklund, Peter C</creator><creator>Yun, Jong K</creator><creator>Kester, Mark</creator><creator>Adair, James H</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20081201</creationdate><title>Encapsulation of Organic Molecules in Calcium Phosphate Nanocomposite Particles for Intracellular Imaging and Drug Delivery</title><author>Morgan, Thomas T ; Muddana, Hari S ; Altinoǧlu, Erhan İ ; Rouse, Sarah M ; Tabaković, Amra ; Tabouillot, Tristan ; Russin, Timothy J ; Shanmugavelandy, Sriram S ; Butler, Peter J ; Eklund, Peter C ; Yun, Jong K ; Kester, Mark ; Adair, James H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a531t-7435390c22102bb2b9809f9c1e30390ebe9d0a73f3fa4499fec7d75a43610add3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Calcium Phosphates - chemistry</topic><topic>Cattle</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>Diffusion in nanoscale solids</topic><topic>Diffusion in solids</topic><topic>Drug Carriers</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. 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The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>19367837</pmid><doi>10.1021/nl8019888</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nano letters, 2008-12, Vol.8 (12), p.4108-4115 |
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| subjects | Animals Biological and medical sciences Biotechnology Calcium Phosphates - chemistry Cattle Condensed matter: structure, mechanical and thermal properties Cross-disciplinary physics: materials science rheology Diffusion in nanoscale solids Diffusion in solids Drug Carriers Exact sciences and technology Fundamental and applied biological sciences. Psychology Materials science Methods. Procedures. Technologies Microscopy, Electron, Transmission Nanocomposites Nanocrystalline materials Nanoscale materials and structures: fabrication and characterization Organic Chemicals - chemistry Others Physics Transport properties of condensed matter (nonelectronic) Various methods and equipments |
| title | Encapsulation of Organic Molecules in Calcium Phosphate Nanocomposite Particles for Intracellular Imaging and Drug Delivery |
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