Multiplexed Cancer Biomarker Detection Using Quartz-Based Photonic Crystal Surfaces

A photonic crystal (PC) surface is demonstrated as a high-sensitivity platform for detection of a panel of 21 cancer biomarker antigens using a sandwich enzyme-linked immunosorbent assay (ELISA) microarray format. A quartz-based PC structure fabricated by nanoimprint lithography, selected for its lo...

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Veröffentlicht in:	Analytical Chemistry, 84(2):1126-1133 84(2):1126-1133, 2012-01, Vol.84 (2), p.1126-1133
Hauptverfasser:	Huang, Cheng-Sheng, Chaudhery, Vikram, Pokhriyal, Anusha, George, Sherine, Polans, James, Lu, Meng, Tan, Ruimin, Zangar, Richard C, Cunningham, Brian T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry antibodies Antibodies, Immobilized - chemistry Antibodies, Immobilized - metabolism Antigens Biomarkers Biomarkers, Tumor - analysis Biosensing Techniques Cancer Chemistry Crystals Enzyme-Linked Immunosorbent Assay Exact sciences and technology fluorescence fluorescence microscopy General, instrumentation Humans image analysis Immunoassay Lasers lighting microarray technology Microscopy Miscellaneous neoplasms Neoplasms - immunology Neoplasms - metabolism photonics Photons Protein Array Analysis Quartz Spectrometric and optical methods Spectrometry, Fluorescence Surface Properties
Online-Zugang:	Volltext
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