Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions
The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically tran...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1982-01, Vol.10 (2), p.577-592 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 592 |
|---|---|
| container_issue | 2 |
| container_start_page | 577 |
| container_title | Nucleic acids research |
| container_volume | 10 |
| creator | van der Putten, Herman Quint, Wim Verma, Inder M. Berns, Anton |
| description | The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically transmitted sequences of M-MuLV, AKR-MuLV and the c-mos gene are all in DNase I resistant chromatin conformations in M-MuLV-induced tumors. Each M-MuLV-induced tumor contained at least one somatically acquired integrated recombinant MuLV genome that displayed two main characteristic features of active chromatin: a) a configuration hypersensitive to DNase I, and b) extensive hypomethylation. DNase I hypersensitive sites were mapped at the junction of cellular sequences and the 5′-viral large terminal repeat (LTR). Expression of a recombinant MuLV seems therefore to be a necessary feature to maintain the transformed state. |
| doi_str_mv | 10.1093/nar/10.2.577 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_326159</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15387873</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-316d5257a91a01da6bacaf9f3e57e7f0848e57e81ac9fa18dfbce798b62f51db3</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EKkvhxhUpJ06k9Uf8hcQBltIiLXABgXoxjjNpTRN7sZNV-9_jZVcrOPU0T_N-M7LnIfSc4BOCNTsNNp0WTU-4lA_QgjBB60YL-hAtMMO8JrhRj9GTnH9hTBrCmyN0JKhUDaUL9PNTHGKAu2qckw9QDTDfwOhttfFpzrUP3eygq6Z5jCm_rhK4OLY-2DBV6xT_QpArHyrrJr-Byl2nONqpNBJc-RjyU_Sot0OGZ_t6jL59OPu6vKhXX84_Lt-uatdINdWMiI5TLq0mFpPOitY62-ueAZcge6watVWKWKd7S1TXtw6kVq2gPSddy47Rm93e9dyO0DkIU7KDWSc_2nRnovXmfyf4a3MVN4ZRQbgu8y_38yn-niFPZvTZwTDYAHHORjItdUPFvSApp-dM4_tBzpRUkhXw1Q50KeacoD-8mmCzjdiUiLeamhJxwV_8-9MDvM-0-PXO93mC24Nt040RkkluLn5cmverd_r75_NLs2R_AE7ZtYQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15387873</pqid></control><display><type>article</type><title>Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions</title><source>Oxford University Press Journals Digital Archive legacy</source><source>MEDLINE</source><source>PubMed Central</source><creator>van der Putten, Herman ; Quint, Wim ; Verma, Inder M. ; Berns, Anton</creator><creatorcontrib>van der Putten, Herman ; Quint, Wim ; Verma, Inder M. ; Berns, Anton</creatorcontrib><description>The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically transmitted sequences of M-MuLV, AKR-MuLV and the c-mos gene are all in DNase I resistant chromatin conformations in M-MuLV-induced tumors. Each M-MuLV-induced tumor contained at least one somatically acquired integrated recombinant MuLV genome that displayed two main characteristic features of active chromatin: a) a configuration hypersensitive to DNase I, and b) extensive hypomethylation. DNase I hypersensitive sites were mapped at the junction of cellular sequences and the 5′-viral large terminal repeat (LTR). Expression of a recombinant MuLV seems therefore to be a necessary feature to maintain the transformed state.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/10.2.577</identifier><identifier>PMID: 6278422</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Akr murine leukemia virus ; AKR murine leukemia virus - genetics ; Animals ; Cell Line ; Cell Nucleus - physiology ; Cell Transformation, Neoplastic ; Chromatin - physiology ; Deoxyribonuclease I ; Deoxyribonucleases ; DNA Restriction Enzymes ; DNA, Recombinant - analysis ; Endonucleases ; Leukemia, Experimental - microbiology ; Methylation ; Mice ; Mice, Inbred BALB C ; Moloney murine leukemia virus - genetics ; Murine leukemia virus</subject><ispartof>Nucleic acids research, 1982-01, Vol.10 (2), p.577-592</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-316d5257a91a01da6bacaf9f3e57e7f0848e57e81ac9fa18dfbce798b62f51db3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC326159/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC326159/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6278422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Putten, Herman</creatorcontrib><creatorcontrib>Quint, Wim</creatorcontrib><creatorcontrib>Verma, Inder M.</creatorcontrib><creatorcontrib>Berns, Anton</creatorcontrib><title>Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically transmitted sequences of M-MuLV, AKR-MuLV and the c-mos gene are all in DNase I resistant chromatin conformations in M-MuLV-induced tumors. Each M-MuLV-induced tumor contained at least one somatically acquired integrated recombinant MuLV genome that displayed two main characteristic features of active chromatin: a) a configuration hypersensitive to DNase I, and b) extensive hypomethylation. DNase I hypersensitive sites were mapped at the junction of cellular sequences and the 5′-viral large terminal repeat (LTR). Expression of a recombinant MuLV seems therefore to be a necessary feature to maintain the transformed state.</description><subject>Akr murine leukemia virus</subject><subject>AKR murine leukemia virus - genetics</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Nucleus - physiology</subject><subject>Cell Transformation, Neoplastic</subject><subject>Chromatin - physiology</subject><subject>Deoxyribonuclease I</subject><subject>Deoxyribonucleases</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Recombinant - analysis</subject><subject>Endonucleases</subject><subject>Leukemia, Experimental - microbiology</subject><subject>Methylation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Moloney murine leukemia virus - genetics</subject><subject>Murine leukemia virus</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhxhUpJ06k9Uf8hcQBltIiLXABgXoxjjNpTRN7sZNV-9_jZVcrOPU0T_N-M7LnIfSc4BOCNTsNNp0WTU-4lA_QgjBB60YL-hAtMMO8JrhRj9GTnH9hTBrCmyN0JKhUDaUL9PNTHGKAu2qckw9QDTDfwOhttfFpzrUP3eygq6Z5jCm_rhK4OLY-2DBV6xT_QpArHyrrJr-Byl2nONqpNBJc-RjyU_Sot0OGZ_t6jL59OPu6vKhXX84_Lt-uatdINdWMiI5TLq0mFpPOitY62-ueAZcge6watVWKWKd7S1TXtw6kVq2gPSddy47Rm93e9dyO0DkIU7KDWSc_2nRnovXmfyf4a3MVN4ZRQbgu8y_38yn-niFPZvTZwTDYAHHORjItdUPFvSApp-dM4_tBzpRUkhXw1Q50KeacoD-8mmCzjdiUiLeamhJxwV_8-9MDvM-0-PXO93mC24Nt040RkkluLn5cmverd_r75_NLs2R_AE7ZtYQ</recordid><startdate>19820122</startdate><enddate>19820122</enddate><creator>van der Putten, Herman</creator><creator>Quint, Wim</creator><creator>Verma, Inder M.</creator><creator>Berns, Anton</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7T5</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820122</creationdate><title>Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions</title><author>van der Putten, Herman ; Quint, Wim ; Verma, Inder M. ; Berns, Anton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-316d5257a91a01da6bacaf9f3e57e7f0848e57e81ac9fa18dfbce798b62f51db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Akr murine leukemia virus</topic><topic>AKR murine leukemia virus - genetics</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Nucleus - physiology</topic><topic>Cell Transformation, Neoplastic</topic><topic>Chromatin - physiology</topic><topic>Deoxyribonuclease I</topic><topic>Deoxyribonucleases</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Recombinant - analysis</topic><topic>Endonucleases</topic><topic>Leukemia, Experimental - microbiology</topic><topic>Methylation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Moloney murine leukemia virus - genetics</topic><topic>Murine leukemia virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Putten, Herman</creatorcontrib><creatorcontrib>Quint, Wim</creatorcontrib><creatorcontrib>Verma, Inder M.</creatorcontrib><creatorcontrib>Berns, Anton</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Putten, Herman</au><au>Quint, Wim</au><au>Verma, Inder M.</au><au>Berns, Anton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1982-01-22</date><risdate>1982</risdate><volume>10</volume><issue>2</issue><spage>577</spage><epage>592</epage><pages>577-592</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically transmitted sequences of M-MuLV, AKR-MuLV and the c-mos gene are all in DNase I resistant chromatin conformations in M-MuLV-induced tumors. Each M-MuLV-induced tumor contained at least one somatically acquired integrated recombinant MuLV genome that displayed two main characteristic features of active chromatin: a) a configuration hypersensitive to DNase I, and b) extensive hypomethylation. DNase I hypersensitive sites were mapped at the junction of cellular sequences and the 5′-viral large terminal repeat (LTR). Expression of a recombinant MuLV seems therefore to be a necessary feature to maintain the transformed state.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6278422</pmid><doi>10.1093/nar/10.2.577</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1982-01, Vol.10 (2), p.577-592 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_326159 |
| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Akr murine leukemia virus AKR murine leukemia virus - genetics Animals Cell Line Cell Nucleus - physiology Cell Transformation, Neoplastic Chromatin - physiology Deoxyribonuclease I Deoxyribonucleases DNA Restriction Enzymes DNA, Recombinant - analysis Endonucleases Leukemia, Experimental - microbiology Methylation Mice Mice, Inbred BALB C Moloney murine leukemia virus - genetics Murine leukemia virus |
| title | Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T09%3A05%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Moloney%20murine%20leukemia%20virus-induced%20tumors:%20recombinant%20proviruses%20in%20active%20chromatin%20regions&rft.jtitle=Nucleic%20acids%20research&rft.au=van%20der%20Putten,%20Herman&rft.date=1982-01-22&rft.volume=10&rft.issue=2&rft.spage=577&rft.epage=592&rft.pages=577-592&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/10.2.577&rft_dat=%3Cproquest_pubme%3E15387873%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15387873&rft_id=info:pmid/6278422&rfr_iscdi=true |