Effects of DNA Methylation on the Structure of Nucleosomes
Nucleosomes are the fundamental packing units of the eukaryotic genome. Understanding the dynamic structure of a nucleosome is a key to the elucidation of genome packaging in eukaryotes, which is tied to the mechanisms of gene regulation. CpG methylation of DNA is an epigenetic modification associat...
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| Veröffentlicht in: | Journal of the American Chemical Society 2012-01, Vol.134 (1), p.173-175 |
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| container_title | Journal of the American Chemical Society |
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| creator | Lee, Ju Yeon Lee, Tae-Hee |
| description | Nucleosomes are the fundamental packing units of the eukaryotic genome. Understanding the dynamic structure of a nucleosome is a key to the elucidation of genome packaging in eukaryotes, which is tied to the mechanisms of gene regulation. CpG methylation of DNA is an epigenetic modification associated with the inactivation of transcription and the formation of a repressive chromatin structure. Unraveling the changes in the structure of nucleosomes upon CpG methylation is an essential step toward the understanding of the mechanisms of gene repression and silencing by CpG methylation. Here we report single-molecule and ensemble fluorescence studies showing how the structure of a nucleosome is affected by CpG methylation. The results indicate that CpG methylation induces tighter wrapping of DNA around the histone core accompanied by a topology change. These findings suggest that changes in the physical properties of nucleosomes induced upon CpG methylation may contribute directly to the formation of a repressive chromatin structure. |
| doi_str_mv | 10.1021/ja210273w |
| format | Article |
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Understanding the dynamic structure of a nucleosome is a key to the elucidation of genome packaging in eukaryotes, which is tied to the mechanisms of gene regulation. CpG methylation of DNA is an epigenetic modification associated with the inactivation of transcription and the formation of a repressive chromatin structure. Unraveling the changes in the structure of nucleosomes upon CpG methylation is an essential step toward the understanding of the mechanisms of gene repression and silencing by CpG methylation. Here we report single-molecule and ensemble fluorescence studies showing how the structure of a nucleosome is affected by CpG methylation. The results indicate that CpG methylation induces tighter wrapping of DNA around the histone core accompanied by a topology change. These findings suggest that changes in the physical properties of nucleosomes induced upon CpG methylation may contribute directly to the formation of a repressive chromatin structure.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja210273w</identifier><identifier>PMID: 22148575</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Base Sequence ; CpG Islands - genetics ; DNA - genetics ; DNA - metabolism ; DNA Methylation ; Fluorescence Polarization ; Models, Molecular ; Molecular Sequence Data ; Nucleosomes - chemistry ; Nucleosomes - metabolism ; Protein Conformation</subject><ispartof>Journal of the American Chemical Society, 2012-01, Vol.134 (1), p.173-175</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2011 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a470t-26dc441fc0fdd2c06557c16abc348cc116df1a8e8fe1403e7cad663a9051dcb63</citedby><cites>FETCH-LOGICAL-a470t-26dc441fc0fdd2c06557c16abc348cc116df1a8e8fe1403e7cad663a9051dcb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja210273w$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja210273w$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22148575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ju Yeon</creatorcontrib><creatorcontrib>Lee, Tae-Hee</creatorcontrib><title>Effects of DNA Methylation on the Structure of Nucleosomes</title><title>Journal of the American Chemical Society</title><addtitle>J. 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These findings suggest that changes in the physical properties of nucleosomes induced upon CpG methylation may contribute directly to the formation of a repressive chromatin structure.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>CpG Islands - genetics</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>DNA Methylation</subject><subject>Fluorescence Polarization</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Nucleosomes - chemistry</subject><subject>Nucleosomes - metabolism</subject><subject>Protein Conformation</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1LAzEQhoMotlYP_gHZi4iH1Uw2H1sPQqn1A2o9qOeQZhO7ZbvRJKv037ultSgIA8MwD-8MD0LHgC8AE7icK9J2kX3toC4wglMGhO-iLsaYpCLnWQcdhDBvR0py2EcdQoDmTLAuuhpZa3QMibPJzWSQPJo4W1Yqlq5O2oozkzxH3-jYeLNiJo2ujAtuYcIh2rOqCuZo03vo9Xb0MrxPx093D8PBOFVU4JgSXmhKwWpsi4JozBkTGria6ozmWgPwwoLKTW4NUJwZoVXBeab6mEGhpzzroet17nszXZhCmzp6Vcl3Xy6UX0qnSvl3U5cz-eY-ZUaYyPgq4GwT4N1HY0KUizJoU1WqNq4Jsg8cKBWAW_J8TWrvQvDGbq8AlivVcqu6ZU9-v7Ulf9y2wOkaUDrIuWt83Vr6J-gbbweFzA</recordid><startdate>20120111</startdate><enddate>20120111</enddate><creator>Lee, Ju Yeon</creator><creator>Lee, Tae-Hee</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120111</creationdate><title>Effects of DNA Methylation on the Structure of Nucleosomes</title><author>Lee, Ju Yeon ; Lee, Tae-Hee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a470t-26dc441fc0fdd2c06557c16abc348cc116df1a8e8fe1403e7cad663a9051dcb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>CpG Islands - genetics</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>DNA Methylation</topic><topic>Fluorescence Polarization</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Nucleosomes - chemistry</topic><topic>Nucleosomes - metabolism</topic><topic>Protein Conformation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ju Yeon</creatorcontrib><creatorcontrib>Lee, Tae-Hee</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ju Yeon</au><au>Lee, Tae-Hee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of DNA Methylation on the Structure of Nucleosomes</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Base Sequence CpG Islands - genetics DNA - genetics DNA - metabolism DNA Methylation Fluorescence Polarization Models, Molecular Molecular Sequence Data Nucleosomes - chemistry Nucleosomes - metabolism Protein Conformation |
| title | Effects of DNA Methylation on the Structure of Nucleosomes |
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