Importance of the Protein Framework for Catalytic Activity of [FeFe]-Hydrogenases

The active center (H-cluster) of [FeFe]-hydrogenases is embedded into a hydrophobic pocket within the protein. We analyzed several amino acids, located in the vicinity of this niche, by site-directed mutagenesis of the [FeFe]-hydrogenases from Clostridium pasteurianum (CpI) and Chlamydomonas reinhar...

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Veröffentlicht in:	The Journal of biological chemistry 2012-01, Vol.287 (2), p.1489-1499
Hauptverfasser:	Knörzer, Philipp, Silakov, Alexey, Foster, Carina E., Armstrong, Fraser A., Lubitz, Wolfgang, Happe, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Biophysics Catalysis Chlamydomonas Chlamydomonas reinhardtii - enzymology Chlamydomonas reinhardtii - genetics Clostridium - enzymology Clostridium - genetics Desulfovibrio desulfuricans - enzymology Desulfovibrio desulfuricans - genetics Electrochemistry Electron Spin Resonance Spectroscopy Hydrogenase Hydrogenase - chemistry Hydrogenase - genetics Kinetics Metalloenzymes Metalloproteins Molecular Biophysics Mutagenesis, Site-Directed Plant Proteins - chemistry Plant Proteins - genetics Protein Conformation Protein-Metal Ion Interaction Site-directed Mutagenesis Spectroscopy Spectroscopy, Fourier Transform Infrared
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