Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes
Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy. In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA...
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| Veröffentlicht in: | Experimental diabetes research 2011-01, Vol.2011 (2011), p.1-11 |
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| container_title | Experimental diabetes research |
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| creator | Rybczyńska, Apolonia Pikuła, Michał Barczyńska, Anna Maryn, Anna Latawiec, Elżbieta Lewko, Barbara Zieliński, Maciej |
| description | Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy. In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days. Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity. High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation. Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR. In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner. Posttranslational events may affect AR activity independent of enzyme protein amount. Activation of AR in podocytes may be implicated in diabetic podocytopathy. |
| doi_str_mv | 10.1155/2011/278963 |
| format | Article |
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In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days. Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity. High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation. Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR. In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner. Posttranslational events may affect AR activity independent of enzyme protein amount. Activation of AR in podocytes may be implicated in diabetic podocytopathy.</description><identifier>ISSN: 2314-6745</identifier><identifier>ISSN: 1687-5214</identifier><identifier>EISSN: 2314-6753</identifier><identifier>EISSN: 1687-5303</identifier><identifier>DOI: 10.1155/2011/278963</identifier><identifier>PMID: 22253613</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Aldehyde Reductase - genetics ; Aldehyde Reductase - metabolism ; Animals ; Cells, Cultured ; Diabetic Nephropathies - enzymology ; Diabetic Nephropathies - genetics ; Diabetic Nephropathies - metabolism ; Dose-Response Relationship, Drug ; Enzyme Activation - drug effects ; Gene Expression Regulation, Enzymologic - drug effects ; Glucose - metabolism ; Glucose - pharmacology ; Humans ; Mice ; Osmolar Concentration ; Podocytes - drug effects ; Podocytes - metabolism ; Podocytes - physiology ; Time Factors</subject><ispartof>Experimental diabetes research, 2011-01, Vol.2011 (2011), p.1-11</ispartof><rights>Copyright © 2011 Barbara Lewko et al.</rights><rights>Copyright © 2011 Barbara Lewko et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-f44eff8ac4895867434afe81463ca7a14e8be001beee5119f64fe70f4879fe933</citedby><cites>FETCH-LOGICAL-c438t-f44eff8ac4895867434afe81463ca7a14e8be001beee5119f64fe70f4879fe933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255165/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255165/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22253613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kurtz, Theodore W.</contributor><creatorcontrib>Rybczyńska, Apolonia</creatorcontrib><creatorcontrib>Pikuła, Michał</creatorcontrib><creatorcontrib>Barczyńska, Anna</creatorcontrib><creatorcontrib>Maryn, Anna</creatorcontrib><creatorcontrib>Latawiec, Elżbieta</creatorcontrib><creatorcontrib>Lewko, Barbara</creatorcontrib><creatorcontrib>Zieliński, Maciej</creatorcontrib><title>Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes</title><title>Experimental diabetes research</title><addtitle>Exp Diabetes Res</addtitle><description>Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy. In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days. Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity. High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation. Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR. In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner. Posttranslational events may affect AR activity independent of enzyme protein amount. Activation of AR in podocytes may be implicated in diabetic podocytopathy.</description><subject>Aldehyde Reductase - genetics</subject><subject>Aldehyde Reductase - metabolism</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Diabetic Nephropathies - enzymology</subject><subject>Diabetic Nephropathies - genetics</subject><subject>Diabetic Nephropathies - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activation - drug effects</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Glucose - metabolism</subject><subject>Glucose - pharmacology</subject><subject>Humans</subject><subject>Mice</subject><subject>Osmolar Concentration</subject><subject>Podocytes - drug effects</subject><subject>Podocytes - metabolism</subject><subject>Podocytes - physiology</subject><subject>Time Factors</subject><issn>2314-6745</issn><issn>1687-5214</issn><issn>2314-6753</issn><issn>1687-5303</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc9LwzAUx4MoTuZO3qVnpa5pkja9CGPqFCaToeeQJS9bJGtHf0z635tSHfPmu7wH38_75vENQlc4usOYsXEcYTyOU54l5ARdxATTMEkZOT3MlA3QqKo-I18ZyTjj52gQxzEjCSYXSC-qbeFkaes2kLkOZq5RRQXBgzUGSshrK51rgyWsGydrCCZOd_ISdKNq6aeJqu2-27Z5MG1c3ZSgg9ei8dJboQvV1lBdojMjXQWjnz5EH0-P79PncL6YvUwn81BRwuvQUArGcKkozxj3pxMqDXBME6JkKjEFvoIowisAYBhnJqEG0shQnmYGMkKG6L733TWrLWjlzy-lE7vSbmXZikJa8VfJ7Uasi70gMWM4Yd7gtjdQZVFVJZjDLo5El7fo8hZ93p6-Pn7uwP6m64GbHtjYXMsv-z838AgYeQTHafen339qlDE</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Rybczyńska, Apolonia</creator><creator>Pikuła, Michał</creator><creator>Barczyńska, Anna</creator><creator>Maryn, Anna</creator><creator>Latawiec, Elżbieta</creator><creator>Lewko, Barbara</creator><creator>Zieliński, Maciej</creator><general>Hindawi Publishing Corporation</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes</title><author>Rybczyńska, Apolonia ; Pikuła, Michał ; Barczyńska, Anna ; Maryn, Anna ; Latawiec, Elżbieta ; Lewko, Barbara ; Zieliński, Maciej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-f44eff8ac4895867434afe81463ca7a14e8be001beee5119f64fe70f4879fe933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aldehyde Reductase - genetics</topic><topic>Aldehyde Reductase - metabolism</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Diabetic Nephropathies - enzymology</topic><topic>Diabetic Nephropathies - genetics</topic><topic>Diabetic Nephropathies - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activation - drug effects</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Glucose - metabolism</topic><topic>Glucose - pharmacology</topic><topic>Humans</topic><topic>Mice</topic><topic>Osmolar Concentration</topic><topic>Podocytes - drug effects</topic><topic>Podocytes - metabolism</topic><topic>Podocytes - physiology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rybczyńska, Apolonia</creatorcontrib><creatorcontrib>Pikuła, Michał</creatorcontrib><creatorcontrib>Barczyńska, Anna</creatorcontrib><creatorcontrib>Maryn, Anna</creatorcontrib><creatorcontrib>Latawiec, Elżbieta</creatorcontrib><creatorcontrib>Lewko, Barbara</creatorcontrib><creatorcontrib>Zieliński, Maciej</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental diabetes research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rybczyńska, Apolonia</au><au>Pikuła, Michał</au><au>Barczyńska, Anna</au><au>Maryn, Anna</au><au>Latawiec, Elżbieta</au><au>Lewko, Barbara</au><au>Zieliński, Maciej</au><au>Kurtz, Theodore W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes</atitle><jtitle>Experimental diabetes research</jtitle><addtitle>Exp Diabetes Res</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6745</issn><issn>1687-5214</issn><eissn>2314-6753</eissn><eissn>1687-5303</eissn><abstract>Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy. In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days. Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity. High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation. Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR. In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner. Posttranslational events may affect AR activity independent of enzyme protein amount. Activation of AR in podocytes may be implicated in diabetic podocytopathy.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>22253613</pmid><doi>10.1155/2011/278963</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aldehyde Reductase - genetics Aldehyde Reductase - metabolism Animals Cells, Cultured Diabetic Nephropathies - enzymology Diabetic Nephropathies - genetics Diabetic Nephropathies - metabolism Dose-Response Relationship, Drug Enzyme Activation - drug effects Gene Expression Regulation, Enzymologic - drug effects Glucose - metabolism Glucose - pharmacology Humans Mice Osmolar Concentration Podocytes - drug effects Podocytes - metabolism Podocytes - physiology Time Factors |
| title | Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes |
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