Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest
Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid,...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-12 |
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| creator | Yu, Bo Yang, Hong Yi, Fei Al Shawi, Ali Rasul, Azhar Khan, Muhammad Ma, Tonghui |
| description | Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase. |
| doi_str_mv | 10.1155/2012/703034 |
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| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3246879</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2060808720</sourcerecordid><originalsourceid>FETCH-LOGICAL-c466t-77ea6f529e85f8216cda2c66a2685eb7674f91200ff19010520dec53a5a73c3f3</originalsourceid><addsrcrecordid>eNqNkc2LFDEQxYMo7rp68i4BD4IyTqXS-eiLMAw6rqy4Bxe8hUw62c7S0xmTbsX_3iyzDqsnT1VU_Xi8qkfIcwZvGRNiicBwqYADbx6QU6YatmhQ64fHXn07IU9KuQHAVin1mJwgIpMg9Cm5_GSdTyV2caTnYzc7X-hqn_ZTnRVah1da0c0Q03awZUo7S9d-GAqd-pzm655ucPmZXva2eLrK2ZfpKXkU7FD8s7t6Rq4-vP-6_ri4-LI5X68uFq6Rcloo5a0MAluvRdDVjessOiktSi38VknVhJYhQAisBQYCofNOcCus4o4HfkbeHXT383bnO-fHKdvB7HPc2fzLJBvN35sx9uY6_TAcG6lVWwVe3Qnk9H2uzs0uFlePs6NPczEtazjUD4pKvvyHvElzHut1BkGCBq0QKvXmQLmcSsk-HL0wMLdBmdugzCGoSr-4b__I_kmmAq8PQB_Hzv6M_6fmK-KDvQejbrTmvwFVNKLh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2060808720</pqid></control><display><type>article</type><title>Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest</title><source>Wiley Online Library</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Yu, Bo ; Yang, Hong ; Yi, Fei ; Al Shawi, Ali ; Rasul, Azhar ; Khan, Muhammad ; Ma, Tonghui</creator><contributor>Längler, Alfred ; Alfred Längler</contributor><creatorcontrib>Yu, Bo ; Yang, Hong ; Yi, Fei ; Al Shawi, Ali ; Rasul, Azhar ; Khan, Muhammad ; Ma, Tonghui ; Längler, Alfred ; Alfred Längler</creatorcontrib><description>Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2012/703034</identifier><identifier>PMID: 22216058</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Apoptosis ; Biological activity ; Brain cancer ; Cancer ; Caspase ; Caspase-3 ; Cell cycle ; Chromatography ; Cytochrome ; Cytochrome c ; DNA damage ; Ethanol ; Flow cytometry ; Glioblastoma ; Glioblastoma cells ; Herbal medicine ; Leaves ; Mass spectrometry ; Medicinal plants ; Membrane potential ; Mitochondria ; NMR ; Nuclear magnetic resonance ; p53 Protein ; Scientific imaging</subject><ispartof>Evidence-based complementary and alternative medicine, 2012-01, Vol.2012 (2012), p.1-12</ispartof><rights>Copyright © 2012 Muhammad Khan et al.</rights><rights>Copyright © 2012 Muhammad Khan et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2012 Muhammad Khan et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-77ea6f529e85f8216cda2c66a2685eb7674f91200ff19010520dec53a5a73c3f3</citedby><cites>FETCH-LOGICAL-c466t-77ea6f529e85f8216cda2c66a2685eb7674f91200ff19010520dec53a5a73c3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246879/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246879/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22216058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Längler, Alfred</contributor><contributor>Alfred Längler</contributor><creatorcontrib>Yu, Bo</creatorcontrib><creatorcontrib>Yang, Hong</creatorcontrib><creatorcontrib>Yi, Fei</creatorcontrib><creatorcontrib>Al Shawi, Ali</creatorcontrib><creatorcontrib>Rasul, Azhar</creatorcontrib><creatorcontrib>Khan, Muhammad</creatorcontrib><creatorcontrib>Ma, Tonghui</creatorcontrib><title>Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Artemisia argyi is a widely used medicinal plant in China. The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase.</description><subject>Apoptosis</subject><subject>Biological activity</subject><subject>Brain cancer</subject><subject>Cancer</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell cycle</subject><subject>Chromatography</subject><subject>Cytochrome</subject><subject>Cytochrome c</subject><subject>DNA damage</subject><subject>Ethanol</subject><subject>Flow cytometry</subject><subject>Glioblastoma</subject><subject>Glioblastoma cells</subject><subject>Herbal medicine</subject><subject>Leaves</subject><subject>Mass spectrometry</subject><subject>Medicinal plants</subject><subject>Membrane potential</subject><subject>Mitochondria</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>p53 Protein</subject><subject>Scientific imaging</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkc2LFDEQxYMo7rp68i4BD4IyTqXS-eiLMAw6rqy4Bxe8hUw62c7S0xmTbsX_3iyzDqsnT1VU_Xi8qkfIcwZvGRNiicBwqYADbx6QU6YatmhQ64fHXn07IU9KuQHAVin1mJwgIpMg9Cm5_GSdTyV2caTnYzc7X-hqn_ZTnRVah1da0c0Q03awZUo7S9d-GAqd-pzm655ucPmZXva2eLrK2ZfpKXkU7FD8s7t6Rq4-vP-6_ri4-LI5X68uFq6Rcloo5a0MAluvRdDVjessOiktSi38VknVhJYhQAisBQYCofNOcCus4o4HfkbeHXT383bnO-fHKdvB7HPc2fzLJBvN35sx9uY6_TAcG6lVWwVe3Qnk9H2uzs0uFlePs6NPczEtazjUD4pKvvyHvElzHut1BkGCBq0QKvXmQLmcSsk-HL0wMLdBmdugzCGoSr-4b__I_kmmAq8PQB_Hzv6M_6fmK-KDvQejbrTmvwFVNKLh</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Yu, Bo</creator><creator>Yang, Hong</creator><creator>Yi, Fei</creator><creator>Al Shawi, Ali</creator><creator>Rasul, Azhar</creator><creator>Khan, Muhammad</creator><creator>Ma, Tonghui</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest</title><author>Yu, Bo ; 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The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi. A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi. The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies. Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis. Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3. This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO. Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>22216058</pmid><doi>10.1155/2012/703034</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Biological activity Brain cancer Cancer Caspase Caspase-3 Cell cycle Chromatography Cytochrome Cytochrome c DNA damage Ethanol Flow cytometry Glioblastoma Glioblastoma cells Herbal medicine Leaves Mass spectrometry Medicinal plants Membrane potential Mitochondria NMR Nuclear magnetic resonance p53 Protein Scientific imaging |
| title | Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest |
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