Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support
Phosphotriester solid phase methodology oo a polyamide support [(1980) Nucleic Acids Research, 8, 1081–1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGCGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC)....
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| Veröffentlicht in: | Nucleic acids research 1980-11, Vol.8 (22), p.5193-5206 |
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| container_title | Nucleic acids research |
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| creator | Markham, A.F. Edge, M.D. Atkinson, T.C. Greene, A.R. Heathcliffe, G.R. Newton, C.R. Scanlon, D. |
| description | Phosphotriester solid phase methodology oo a polyamide support [(1980) Nucleic Acids Research, 8, 1081–1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGCGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC). Thus, oligodeoxyribonucleotides comparable in size to those obtainable by solution synthesis are readily accessible using solid phese techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described. |
| doi_str_mv | 10.1093/nar/8.22.5193 |
| format | Article |
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Thus, oligodeoxyribonucleotides comparable in size to those obtainable by solution synthesis are readily accessible using solid phese techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/8.22.5193</identifier><identifier>PMID: 7465412</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Base Sequence ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Indicators and Reagents ; Methods ; Oligodeoxyribonucleotides - chemical synthesis ; Oligonucleotides - chemical synthesis ; Resins, Plant</subject><ispartof>Nucleic acids research, 1980-11, Vol.8 (22), p.5193-5206</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-355651b272590f03d375291657a46e242359c567df26dcf57cc67bb59ba9079b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC324294/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC324294/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7465412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Markham, A.F.</creatorcontrib><creatorcontrib>Edge, M.D.</creatorcontrib><creatorcontrib>Atkinson, T.C.</creatorcontrib><creatorcontrib>Greene, A.R.</creatorcontrib><creatorcontrib>Heathcliffe, G.R.</creatorcontrib><creatorcontrib>Newton, C.R.</creatorcontrib><creatorcontrib>Scanlon, D.</creatorcontrib><title>Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Phosphotriester solid phase methodology oo a polyamide support [(1980) Nucleic Acids Research, 8, 1081–1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGCGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC). Thus, oligodeoxyribonucleotides comparable in size to those obtainable by solution synthesis are readily accessible using solid phese techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described.</description><subject>Base Sequence</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Indicators and Reagents</subject><subject>Methods</subject><subject>Oligodeoxyribonucleotides - chemical synthesis</subject><subject>Oligonucleotides - chemical synthesis</subject><subject>Resins, Plant</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVJSRy3xx4De8ptHX3LOuQQTL4gUGhcWnoZtFqtrWS92ki7Jf73lYlx2lMPo0HMMy8z8yL0heAZwZpddCZezGeUzgTR7AOaECZpybWkR2iCGRYlwXx-gk5TesKYcCL4MTpWXApO6ATBY2h9XfRrk1x-Q8oxRO_S4GKRtt2wdsmnIjRFa-LKFZlehdqF1230VehG27ow-NplpCtM0Yd2azb5X6Sx70McPqGPjWmT-7zPU_T95nq5uCsfvt7eL64eSssFHkomhBSkoooKjRvMaqYE1UQKZbh0lFMmtBVS1Q2VtW2EslaqqhK6MhorXbEpunzT7cdq42rruiGaFvroNyZuIRgP_1Y6v4ZV-A0si2ue-8_3_TG8jHl92PhkXduazoUxgRKc5_uy_4IZ4prLeQbLN9DGkFJ0zWEYgmHnHGTnYA6Uws65zJ_9vcGB3lv1ruezN6-HsonPIFU-F9z9_AWaqh-3S_UI39gfdAOmaQ</recordid><startdate>19801125</startdate><enddate>19801125</enddate><creator>Markham, A.F.</creator><creator>Edge, M.D.</creator><creator>Atkinson, T.C.</creator><creator>Greene, A.R.</creator><creator>Heathcliffe, G.R.</creator><creator>Newton, C.R.</creator><creator>Scanlon, D.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19801125</creationdate><title>Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support</title><author>Markham, A.F. ; Edge, M.D. ; Atkinson, T.C. ; Greene, A.R. ; Heathcliffe, G.R. ; Newton, C.R. ; Scanlon, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-355651b272590f03d375291657a46e242359c567df26dcf57cc67bb59ba9079b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Base Sequence</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Indicators and Reagents</topic><topic>Methods</topic><topic>Oligodeoxyribonucleotides - chemical synthesis</topic><topic>Oligonucleotides - chemical synthesis</topic><topic>Resins, Plant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markham, A.F.</creatorcontrib><creatorcontrib>Edge, M.D.</creatorcontrib><creatorcontrib>Atkinson, T.C.</creatorcontrib><creatorcontrib>Greene, A.R.</creatorcontrib><creatorcontrib>Heathcliffe, G.R.</creatorcontrib><creatorcontrib>Newton, C.R.</creatorcontrib><creatorcontrib>Scanlon, D.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markham, A.F.</au><au>Edge, M.D.</au><au>Atkinson, T.C.</au><au>Greene, A.R.</au><au>Heathcliffe, G.R.</au><au>Newton, C.R.</au><au>Scanlon, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1980-11-25</date><risdate>1980</risdate><volume>8</volume><issue>22</issue><spage>5193</spage><epage>5206</epage><pages>5193-5206</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Phosphotriester solid phase methodology oo a polyamide support [(1980) Nucleic Acids Research, 8, 1081–1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGCGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC). Thus, oligodeoxyribonucleotides comparable in size to those obtainable by solution synthesis are readily accessible using solid phese techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>7465412</pmid><doi>10.1093/nar/8.22.5193</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1980-11, Vol.8 (22), p.5193-5206 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_324294 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Base Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Indicators and Reagents Methods Oligodeoxyribonucleotides - chemical synthesis Oligonucleotides - chemical synthesis Resins, Plant |
| title | Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support |
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